Strain Name:
C57BL/6J-MtgxR4529Btlr/Mmmh
Stock Number:
041592-MU
Citation ID:
RRID:MMRRC_041592-MU
Other Names:
R4529 (G1), C57BL/6J-MtgxR4529Btlr
Major Collection:

Gene Information

Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: Emr1, TM7LN3, EGF-TM7, F4/80, DD7A5-7, Ly71
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, 2610202A04Rik, D16Ium21e, D16Ium21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22129
Homologene: 2487
Parp1
Name: poly (ADP-ribose) polymerase family, member 1
Synonyms: 5830444G22Rik, Adprp, sPARP-1, PARP, parp-1, Adprt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11545
HGNC: HGNC:270
Homologene: 1222
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4, Orc4P, mMmORC4, Orc4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: mei2-5, G1-448-15, AKAP450, 5730481H23Rik, repro12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 65246
VEGA: 14
Homologene: 22857
Lin54
Name: lin-54 homolog (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231506
Homologene: 18212
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: D17Ertd710e, NG36, G9a, Bat8, KMT1C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110147
Homologene: 48460
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Themis
Name: thymocyte selection associated
Synonyms: E430004N04Rik, Tsepa, Gasp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210757
Homologene: 72287
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: cytochrome P450 subfamily I, polypeptide 1, P450-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Zfy1
Name: zinc finger protein 1, Y-linked
Synonyms: Zfy-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22767
Homologene: 56456
Plekhm3
Name: pleckstrin homology domain containing, family M, member 3
Synonyms: A230102O09Rik, 9430067K14Rik, Plekhm1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241075
Homologene: 18459
Khdc3
Name: KH domain containing 3, subcortical maternal complex member
Synonyms: FILIA, 2410004A20Rik, ecat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66991
Homologene: 49842
Prss38
Name: protease, serine 38
Synonyms: LOC216797, Gm249
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216797
Homologene: 18831
Stk32a
Name: serine/threonine kinase 32A
Synonyms: YANK1, A930015B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269019
VEGA: 18
Homologene: 65357
Nlrp9a
Name: NLR family, pyrin domain containing 9A
Synonyms: Nalp-theta, D7Ertd565e, Nalp9a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233001
Homologene: 116072
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: Ltbp1L, LTBP-1, 9430031G15Rik, b2b1000Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Ap3b2
Name: adaptor-related protein complex 3, beta 2 subunit
Synonyms: Naptb, beta3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11775
HGNC: HGNC:567
Homologene: 55837
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: CTF18, 6030457M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214901
Homologene: 32532
Apba1
Name: amyloid beta (A4) precursor protein binding, family A, member 1
Synonyms: 6430513E09Rik, Lin-10, X11, Mint1, Mint, X11alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 319924
VEGA: 19
HGNC: HGNC:578
Homologene: 897
Ubqlnl
Name: ubiquilin-like
Synonyms: LOC244179, 4922504M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244179
Homologene: 17034
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: 8430414N03Rik, IGFBP-4ase, PAG1, PAPP-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Olfr786
Name: olfactory receptor 786
Synonyms: MOR111-5, GA_x6K02T2PULF-11116958-11117896
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258542
HGNC: HGNC:8355
Homologene: 133582
Slco1c1
Name: solute carrier organic anion transporter family, member 1c1
Synonyms: Slc21a14, OATP-F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58807
Homologene: 23008
Gsdma2
Name: gasdermin A2
Synonyms: 2210411P14Rik, 2200001G21Rik, Gsdml2, 2210009F20Rik, 2210006M16Rik, Gsdm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76758
Ankrd2
Name: ankyrin repeat domain 2 (stretch responsive muscle)
Synonyms: Arpp, mArpp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56642
VEGA: 19
HGNC: HGNC:495
Homologene: 10568
Lrrc9
Name: leucine rich repeat containing 9
Synonyms: 4930432K16Rik, 4921529O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 78257
Homologene: 12692
Plin4
Name: perilipin 4
Synonyms: S3-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57435
Homologene: 69311
Aldh1a3
Name: aldehyde dehydrogenase family 1, subfamily A3
Synonyms: V1, ALDH6, retinaldehyde dehydrogenase 3, RALDH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56847
HGNC: HGNC:409
Homologene: 68080
Gm12185
Name: predicted gene 12185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 620913
Homologene: 83188
Plbd1
Name: phospholipase B domain containing 1
Synonyms: 1100001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66857
Homologene: 11745
H2-Q6
Name: histocompatibility 2, Q region locus 6
Synonyms: H-2Q6, Qa-6, Qa6, 0610037M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110557
HGNC: HGNC:4931
Homologene: 128352
Pla2g4f
Name: phospholipase A2, group IVF
Synonyms: 4732472I07Rik, Pla2zeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271844
Homologene: 77933
Tmem38a
Name: transmembrane protein 38A
Synonyms: TRIC-A, 1110001E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74166
Homologene: 11449
Inmt
Name: indolethylamine N-methyltransferase
Synonyms: Temt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21743
HGNC: HGNC:6069
Homologene: 81752
Pou3f3
Name: POU domain, class 3, transcription factor 3
Synonyms: Brn1, Otf8, Brn-1, HST011, urehr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18993
HGNC: HGNC:9216
Homologene: 68509
Retreg1
Name: reticulophagy regulator 1
Synonyms: Fam134b, 1810015C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66270
VEGA: 15
Homologene: 10368
Cpa2
Name: carboxypeptidase A2, pancreatic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232680
HGNC: HGNC:2297
Homologene: 37541
Fcamr
Name: Fc receptor, IgA, IgM, high affinity
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 64435
Homologene: 12929
Zfp51
Name: zinc finger protein 51
Synonyms: Zfp-51, zfec12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22709
VEGA: 17
Homologene: 134003
C1qbp
Name: complement component 1, q subcomponent binding protein
Synonyms: D11Wsu182e, HABP1, P32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12261
HGNC: HGNC:1243
Homologene: 31023
Gm5460
Name: predicted gene 5460
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 432838
VEGA: 14
Fam122c
Name: family with sequence similarity 122, member C
Synonyms: 4930527G05Rik, 4930432H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73866
Homologene: 87062
Olfr268-ps1
Name: olfactory receptor 268, pseudogene 1
Synonyms: Gm13931, MOR245-17P, GA_x6K02T2N82Q-12713-13766
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668825
Homologene: 138901
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 42,698,554 bp
  • A to G, chromosome 1 at 64,937,825 bp
  • A to G, chromosome 1 at 130,804,576 bp
  • T to A, chromosome 1 at 180,591,312 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • T to C, chromosome 2 at 111,844,339 bp
  • C to T, chromosome 2 at 120,300,619 bp
  • A to G, chromosome 4 at 65,231,182 bp
  • T to A, chromosome 5 at 4,043,948 bp
  • G to A, chromosome 5 at 100,446,560 bp
  • T to C, chromosome 6 at 30,544,875 bp
  • C to T, chromosome 6 at 32,496,896 bp
  • T to C, chromosome 6 at 55,171,027 bp
  • T to A, chromosome 6 at 136,651,825 bp
  • A to G, chromosome 6 at 141,555,181 bp
  • T to C, chromosome 7 at 26,571,407 bp
  • T to C, chromosome 7 at 66,401,994 bp
  • A to G, chromosome 7 at 81,464,187 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • C to T, chromosome 8 at 72,572,161 bp
  • A to G, chromosome 9 at 57,701,679 bp
  • T to C, chromosome 9 at 73,104,019 bp
  • T to C, chromosome 10 at 28,782,335 bp
  • T to C, chromosome 10 at 129,437,418 bp
  • T to C, chromosome 11 at 48,907,920 bp
  • T to C, chromosome 11 at 48,908,094 bp
  • T to C, chromosome 11 at 59,373,499 bp
  • T to C, chromosome 11 at 70,978,724 bp
  • T to C, chromosome 11 at 98,651,968 bp
  • A to G, chromosome 12 at 72,487,000 bp
  • A to C, chromosome 14 at 34,045,812 bp
  • G to A, chromosome 14 at 70,668,748 bp
  • T to A, chromosome 15 at 25,968,514 bp
  • T to A, chromosome 16 at 94,466,877 bp
  • C to T, chromosome 17 at 21,464,736 bp
  • T to C, chromosome 17 at 25,720,618 bp
  • A to G, chromosome 17 at 34,913,731 bp
  • C to T, chromosome 17 at 35,425,844 bp
  • A to G, chromosome 17 at 56,104,274 bp
  • A to G, chromosome 17 at 57,420,519 bp
  • T to G, chromosome 17 at 75,151,360 bp
  • T to C, chromosome 18 at 43,242,979 bp
  • A to G, chromosome 19 at 23,936,535 bp
  • A to T, chromosome 19 at 42,043,801 bp
  • G to A, chromosome X at 53,293,499 bp
  • A to G, chromosome Y at 726,511 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4529 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041592-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.