Strain Name:
C57BL/6J-MtgxR4542Btlr/Mmmh
Stock Number:
041593-MU
Citation ID:
RRID:MMRRC_041593-MU
Other Names:
R4542 (G1), C57BL/6J-MtgxR4542Btlr
Major Collection:

Strain Information

Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56381
Homologene: 124461
Ssu72
Name: Ssu72 RNA polymerase II CTD phosphatase homolog (yeast)
Synonyms: 2610101M12Rik, 1190002E22Rik, 1500011L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68991
Homologene: 6754
E2f7
Name: E2F transcription factor 7
Synonyms: A630014C11Rik, D10Ertd739e, E2F7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52679
Homologene: 18685
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Stau1
Name: staufen double-stranded RNA binding protein 1
Synonyms: 5830401L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20853
Homologene: 3384
Rfx1
Name: regulatory factor X, 1 (influences HLA class II expression)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19724
HGNC: HGNC:9982
Homologene: 2189
Dph5
Name: diphthamide biosynthesis 5
Synonyms: 2410012M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69740
Homologene: 6471
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: 5230400C17Rik, Pip5k3, PipkIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18711
Homologene: 32115
Zbtb18
Name: zinc finger and BTB domain containing 18
Synonyms: RP58, Zfp238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30928
Homologene: 21276
Or51e2
Name: olfactory receptor family 51 subfamily E member 2
Synonyms: RA1c, PSGR, MOL2.3, 4633402A21Rik, MOR18-2, GA_x6K02T2PBJ9-5459657-5458695, Olfr78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170639
Homologene: 23713
Zfp60
Name: zinc finger protein 60
Synonyms: Mfg-3, Mfg3, 6330516O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22718
Homologene: 137228
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Cab39l
Name: calcium binding protein 39-like
Synonyms: 2810425O13Rik, 1500031K13Rik, MO2L, 4930520C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69008
VEGA: 14
Homologene: 69422
1700017B05Rik
Name: RIKEN cDNA 1700017B05 gene
Synonyms: D9Ertd278e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74211
VEGA: 9
Homologene: 19283
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74499
Homologene: 11542
Nr2f1
Name: nuclear receptor subfamily 2, group F, member 1
Synonyms: COUP-TFI, Erbal3, Tcfcoup1, COUP-TF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13865
HGNC: HGNC:7975
Homologene: 21158
Gja1
Name: gap junction protein, alpha 1
Synonyms: connexin 43, Cx43, alpha 1 connexin, connexin43, Gja-1, Cx43alpha1, Cnx43
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14609
VEGA: 10
HGNC: HGNC:4274
Homologene: 136
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235567
Homologene: 13574
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 637515
Homologene: 19080
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Crip1
Name: cysteine-rich protein 1 (intestinal)
Synonyms: CRP1, Crip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12925
HGNC: HGNC:2360
Homologene: 37465
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Ltbp2
Name: latent transforming growth factor beta binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16997
HGNC: HGNC:6715
Homologene: 369
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171263
Homologene: 128344
Kmt2b
Name: lysine (K)-specific methyltransferase 2B
Synonyms: 2610014H22Rik, Mll2, Wbp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75410
Homologene: 22838
Vmn2r59
Name: vomeronasal 2, receptor 59
Synonyms: EG628444
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628444
Homologene: 129683
Sh2d4a
Name: SH2 domain containing 4A
Synonyms: SH2A, 2210402M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72281
Homologene: 11117
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Bean1
Name: brain expressed, associated with Nedd4, 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 65115
Homologene: 110172
Adcy6
Name: adenylate cyclase 6
Synonyms: AC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11512
VEGA: 15
HGNC: HGNC:237
Homologene: 22400
Rgs22
Name: regulator of G-protein signalling 22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 626596
Homologene: 75047
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: SNS2, NaN, NaT, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Nlrp4c
Name: NLR family, pyrin domain containing 4C
Synonyms: Nalp-alpha, Rnh2, Nalp4c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 83564
Homologene: 75315
Brinp1
Name: bone morphogenic protein/retinoic acid inducible neural specific 1
Synonyms: Dbccr1, Fam5a, Dbc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56710
HGNC: HGNC:2687
Homologene: 8754
Mks1
Name: MKS transition zone complex subunit 1
Synonyms: B8d3, avc6, Meckel syndrome, type 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380718
HGNC: HGNC:7121
Homologene: 9833
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Syne3
Name: spectrin repeat containing, nuclear envelope family member 3
Synonyms: nesprin-3beta, nesprin-3alpha, nesprin-3, 4831426I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 212073
VEGA: 12
Homologene: 17625
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76438
Homologene: 18745
Or5ak23
Name: olfactory receptor family 5 subfamily AK member 23
Synonyms: GA_x6K02T2Q125-46891524-46890580, MOR203-2, Olfr993
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258427
Homologene: 79352
Clgn
Name: calmegin
Synonyms: Cln, 4930459O04Rik, calnexin-t, A2/6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12745
HGNC: HGNC:2060
Homologene: 68392
Cxcr2
Name: chemokine (C-X-C motif) receptor 2
Synonyms: CD128, IL-8Rh, IL-8rb, Gpcr16, IL8RA, Cmkar2, Il8rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12765
HGNC: HGNC:6027
Homologene: 10439
Oxct1
Name: 3-oxoacid CoA transferase 1
Synonyms: 2610008O03Rik, Scot-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67041
HGNC: HGNC:8527
Homologene: 377
Ulbp1
Name: UL16 binding protein 1
Synonyms: MULT1, A430108B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77777
VEGA: 10
Homologene: 108274
Or2d3
Name: olfactory receptor family 2 subfamily D member 3
Synonyms: GA_x6K02T2PBJ9-9276470-9275547, MOR260-8P, Olfr707
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 194433
Homologene: 133899
Nt5dc2
Name: 5'-nucleotidase domain containing 2
Synonyms: 2510015F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70021
VEGA: 14
Homologene: 11290
Slc25a10
Name: solute carrier family 25 (mitochondrial carrier, dicarboxylate transporter), member 10
Synonyms: Dic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27376
Homologene: 6519
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 65,244,430 bp
  • A to G, chromosome 1 at 74,158,529 bp
  • A to G, chromosome 1 at 177,448,666 bp
  • T to C, chromosome 2 at 85,413,943 bp
  • A to G, chromosome 2 at 166,953,261 bp
  • A to T, chromosome 3 at 114,153,739 bp
  • T to C, chromosome 3 at 115,928,625 bp
  • T to C, chromosome 4 at 68,762,092 bp
  • A to G, chromosome 4 at 138,203,417 bp
  • T to C, chromosome 4 at 141,476,786 bp
  • A to G, chromosome 4 at 155,733,477 bp
  • G to A, chromosome 7 at 6,100,827 bp
  • A to G, chromosome 7 at 10,447,430 bp
  • A to G, chromosome 7 at 27,740,961 bp
  • A to G, chromosome 7 at 30,580,259 bp
  • T to C, chromosome 7 at 42,046,073 bp
  • T to C, chromosome 7 at 102,742,643 bp
  • GAACAACAACAA to GAACAACAA, chromosome 7 at 106,891,360 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • T to C, chromosome 8 at 45,041,894 bp
  • A to G, chromosome 8 at 68,346,742 bp
  • A to G, chromosome 8 at 83,420,209 bp
  • G to A, chromosome 8 at 84,090,237 bp
  • T to A, chromosome 8 at 104,210,959 bp
  • A to T, chromosome 9 at 57,256,592 bp
  • CT to C, chromosome 9 at 104,186,805 bp
  • A to T, chromosome 9 at 119,755,134 bp
  • G to A, chromosome 9 at 121,263,638 bp
  • T to C, chromosome 10 at 7,456,570 bp
  • T to C, chromosome 10 at 56,388,052 bp
  • T to C, chromosome 10 at 93,025,129 bp
  • T to A, chromosome 10 at 110,767,123 bp
  • A to C, chromosome 11 at 71,228,325 bp
  • A to T, chromosome 11 at 87,862,010 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • G to A, chromosome 11 at 120,497,981 bp
  • A to T, chromosome 12 at 84,831,819 bp
  • A to T, chromosome 12 at 104,969,244 bp
  • A to G, chromosome 12 at 113,153,489 bp
  • C to T, chromosome 13 at 78,189,821 bp
  • T to A, chromosome 14 at 31,138,138 bp
  • C to A, chromosome 14 at 59,496,902 bp
  • T to C, chromosome 14 at 123,321,477 bp
  • C to T, chromosome 15 at 4,053,726 bp
  • T to A, chromosome 15 at 36,139,868 bp
  • C to G, chromosome 15 at 98,598,988 bp
  • A to G, chromosome 17 at 50,055,231 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4542 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041593-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.