Strain Name:
C57BL/6J-MtgxR4548Btlr/Mmmh
Stock Number:
041594-MU
Citation ID:
RRID:MMRRC_041594-MU
Other Names:
R4548 (G1), C57BL/6J-MtgxR4548Btlr
Major Collection:

Gene Information

Cops3
Name: COP9 signalosome subunit 3
Synonyms: Sgn3, COP9 complex S3, Csn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26572
HGNC: HGNC:2239
Homologene: 2710
Dnajc11
Name: DnaJ heat shock protein family (Hsp40) member C11
Synonyms: E030019A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230935
Homologene: 14558
Polr1c
Name: polymerase (RNA) I polypeptide C
Synonyms: Rpo1-1, RPA40, 40kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20016
Homologene: 3586
Rev1
Name: REV1, DNA directed polymerase
Synonyms: REV1, Rev1l, 1110027I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56210
Homologene: 32309
Anln
Name: anillin, actin binding protein
Synonyms: 1110037A17Rik, Scraps, 2900037I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68743
VEGA: 9
Homologene: 41281
Dis3l2
Name: DIS3 like 3'-5' exoribonuclease 2
Synonyms: 4930429A22Rik, 8030493P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208718
Homologene: 62417
Spata5
Name: spermatogenesis associated 5
Synonyms: 2510048F20Rik, C78064, Spaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 57815
Homologene: 56920
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: Fabph4, Mdgi, Fabph1, Fabp3, H-FABP, Fabph-4, Fabph-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Sfrp4
Name: secreted frizzled-related protein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20379
VEGA: 13
Homologene: 2267
Trappc3
Name: trafficking protein particle complex 3
Synonyms: 1110058K12Rik, Bet3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 27096
Homologene: 6399
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207212
Homologene: 129601
Abca17
Name: ATP-binding cassette, sub-family A (ABC1), member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381072
Homologene: 86807
Adamtsl1
Name: ADAMTS-like 1
Synonyms: 6720426B09Rik, punctin-1, 5930437A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77739
Homologene: 64642
Bcl2l11
Name: BCL2-like 11 (apoptosis facilitator)
Synonyms: Bim, Bod, 1500006F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12125
HGNC: HGNC:994
Homologene: 7643
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237860
Homologene: 14116
Zbed5
Name: zinc finger, BED type containing 5
Synonyms: 2410018M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71970
Homologene: 84838
Akip1
Name: A kinase (PRKA) interacting protein 1
Synonyms: ORF27, ICRFP703B1614Q5.6, D930014E17Rik, ICRFP703N2430Q5.6, BCA3, D7H11orf17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57373
HGNC: HGNC:1170
Homologene: 10765
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: E130113P14Rik, bl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: Aof1, 4632428N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Speer2
Name: spermatogenesis associated glutamate (E)-rich protein 2
Synonyms: SPEER-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224318
Homologene: 69402
Slc7a4
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224022
Homologene: 20883
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268527
Homologene: 8780
Kcnh6
Name: potassium voltage-gated channel, subfamily H (eag-related), member 6
Synonyms: m-erg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192775
Homologene: 32740
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Olfr1100
Name: olfactory receptor 1100
Synonyms: MOR206-4, GA_x6K02T2Q125-48465387-48464422
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258587
Homologene: 34954
Gpr39
Name: G protein-coupled receptor 39
Synonyms: 4933415E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71111
HGNC: HGNC:4496
Homologene: 20380
Cntln
Name: centlein, centrosomal protein
Synonyms: B430108F07Rik, D530005L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 338349
Homologene: 9805
Vmn2r92
Name: vomeronasal 2, receptor 92
Synonyms: EG627111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627111
Homologene: 115024
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 209683
Homologene: 41023
Ercc6l2
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6 like 2
Synonyms: 1700019D06Rik, 0610007P08Rik, 9330134C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76251
Homologene: 32564
Adcy6
Name: adenylate cyclase 6
Synonyms: AC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11512
VEGA: 15
HGNC: HGNC:237
Homologene: 22400
Cfap45
Name: cilia and flagella associated protein 45
Synonyms: 1700028D05Rik, Nesg1, Ccdc19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71870
Homologene: 71837
Olfr155
Name: olfactory receptor 155
Synonyms: GA_x6K02T2N78B-16092200-16091241, OR37A, Olfr37a, mOR37a, MOR262-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29845
HGNC: HGNC:8276
Homologene: 84331
Cfhr3
Name: complement factor H-related 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 624286
Homologene: 138664
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: Nas1, NaSi-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
Ugt2b35
Name: UDP glucuronosyltransferase 2 family, polypeptide B35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243085
Homologene: 128251
Myzap
Name: myocardial zonula adherens protein
Synonyms: Grinl1a7, Gcom1, Myozap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102371
Homologene: 134156
Nemp1
Name: nuclear envelope integral membrane protein 1
Synonyms: Tmem194
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210035
Homologene: 18346
Mrm2
Name: mitochondrial rRNA methyltransferase 2
Synonyms: Ftsj2, 2310037B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68017
Homologene: 5907
Gm15841
Name: predicted gene 15841
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 38,059,194 bp
  • G to A, chromosome 1 at 74,907,612 bp
  • A to T, chromosome 1 at 87,049,671 bp
  • G to A, chromosome 1 at 125,677,991 bp
  • T to G, chromosome 1 at 139,584,913 bp
  • A to G, chromosome 1 at 172,545,108 bp
  • T to C, chromosome 2 at 86,978,670 bp
  • A to G, chromosome 2 at 128,129,646 bp
  • T to A, chromosome 3 at 37,432,027 bp
  • T to A, chromosome 4 at 43,854,834 bp
  • C to A, chromosome 4 at 85,096,842 bp
  • T to C, chromosome 4 at 86,156,724 bp
  • A to G, chromosome 4 at 126,272,751 bp
  • C to T, chromosome 4 at 130,312,452 bp
  • A to G, chromosome 4 at 151,973,617 bp
  • A to T, chromosome 5 at 87,008,275 bp
  • A to G, chromosome 5 at 96,709,895 bp
  • G to A, chromosome 5 at 111,271,224 bp
  • T to A, chromosome 5 at 129,902,851 bp
  • T to C, chromosome 5 at 140,328,496 bp
  • A to G, chromosome 6 at 24,150,719 bp
  • T to C, chromosome 7 at 100,931,129 bp
  • C to A, chromosome 7 at 109,704,986 bp
  • T to C, chromosome 9 at 22,362,888 bp
  • T to C, chromosome 9 at 71,550,246 bp
  • A to G, chromosome 10 at 127,696,344 bp
  • C to T, chromosome 11 at 59,827,845 bp
  • G to A, chromosome 11 at 77,450,184 bp
  • T to C, chromosome 11 at 106,009,049 bp
  • T to C, chromosome 12 at 16,699,675 bp
  • A to G, chromosome 13 at 19,623,766 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • A to G, chromosome 13 at 63,840,912 bp
  • A to G, chromosome 14 at 61,191,938 bp
  • A to G, chromosome 15 at 98,598,659 bp
  • T to C, chromosome 16 at 17,575,345 bp
  • T to C, chromosome 16 at 69,858,849 bp
  • A to T, chromosome 17 at 18,171,316 bp
  • A to T, chromosome 17 at 24,334,271 bp
  • A to T, chromosome 17 at 46,247,809 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041594-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.