Strain Name:
C57BL/6J-MtgxR4553Btlr/Mmmh
Stock Number:
041595-MU
Citation ID:
RRID:MMRRC_041595-MU
Other Names:
R4553 (G1), C57BL/6J-MtgxR4553Btlr
Major Collection:

Gene Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Adra2a
Name: adrenergic receptor, alpha 2a
Synonyms: alpha2A, alpha2A-adrenergic receptor, Adra-2a, Adra-2, alpha2A-AR, alpha(2A)AR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11551
VEGA: 19
HGNC: HGNC:281
Homologene: 47944
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Ezh2
Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Synonyms: Enx-1, Enx1h, KMT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14056
HGNC: HGNC:3527
Homologene: 37926
Tipin
Name: timeless interacting protein
Synonyms: 1110018P21Rik, 1110005A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66131
VEGA: 9
Homologene: 32373
Aen
Name: apoptosis enhancing nuclease
Synonyms: 2700083B06Rik, Isg20l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68048
Homologene: 23382
Adat1
Name: adenosine deaminase, tRNA-specific 1
Synonyms: mADAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 30947
HGNC: HGNC:228
Homologene: 8096
Nog
Name: noggin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18121
HGNC: HGNC:7866
Homologene: 3979
Cd274
Name: CD274 antigen
Synonyms: B7-H1, PD-L1, Pdcd1lg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 60533
Homologene: 8560
Epgn
Name: epithelial mitogen
Synonyms: epigen, 2310069M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71920
Homologene: 19527
Cand2
Name: cullin-associated and neddylation-dissociated 2 (putative)
Synonyms: Tp120b, 2210404G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67088
Homologene: 41657
Sell
Name: selectin, lymphocyte
Synonyms: L-selectin, CD62L, LECAM-1, Ly-22, Ly-m22, Lyam-1, Lnhr, Lyam1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20343
Homologene: 539
Pld1
Name: phospholipase D1
Synonyms: Pld1b, Pld1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212167
Homologene: 45504
Plcb1
Name: phospholipase C, beta 1
Synonyms: 3110043I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18795
Homologene: 22876
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Dppa2
Name: developmental pluripotency associated 2
Synonyms: 2410088E07Rik, ECAT15-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73703
VEGA: 16
Homologene: 79572
Slc8b1
Name: solute carrier family 8 (sodium/lithium/calcium exchanger), member B1
Synonyms: NCKX6, NCLX, Slc24a6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 170756
Homologene: 41602
Xab2
Name: XPA binding protein 2
Synonyms: 0610041O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67439
Homologene: 5738
Cts6
Name: cathepsin 6
Synonyms: 1600022N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 58518
Homologene: 75165
Crat
Name: carnitine acetyltransferase
Synonyms: CARAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12908
HGNC: HGNC:2342
Homologene: 598
Dennd2d
Name: DENN/MADD domain containing 2D
Synonyms: 2010308M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72121
Homologene: 11776
Slc34a1
Name: solute carrier family 34 (sodium phosphate), member 1
Synonyms: renal Na+/Pi transporter, Na/Pi cotransporter, Slc17a2, Npt2, NaPi-IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20505
VEGA: 13
Homologene: 20663
Iqsec2
Name: IQ motif and Sec7 domain 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245666
Homologene: 19407
Mx2
Name: MX dynamin-like GTPase 2
Synonyms: Mx-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17858
Itih4
Name: inter alpha-trypsin inhibitor, heavy chain 4
Synonyms: Itih-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16427
HGNC: HGNC:6169
Homologene: 1670
Ifi35
Name: interferon-induced protein 35
Synonyms: IFP35, 2010008K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70110
HGNC: HGNC:5399
Homologene: 4040
Fam71f2
Name: family with sequence similarity 71, member F2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 245884
Homologene: 52974
Nlrp4e
Name: NLR family, pyrin domain containing 4E
Synonyms: Nalp-epsilon, Nalp4e, 4930406H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 446099
Homologene: 75315
Papolb
Name: poly (A) polymerase beta (testis specific)
Synonyms: TPAP, Papt, Plap-ps, Papola-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56522
Homologene: 121622
Lyve1
Name: lymphatic vessel endothelial hyaluronan receptor 1
Synonyms: lymphatic vessel endothelial HA receptor-1, Lyve-1, 1200012G08Rik, Xlkd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114332
Homologene: 4868
Vmn1r117
Name: vomeronasal 1 receptor 117
Synonyms: Gm8544
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 667262
Homologene: 104166
Armcx5
Name: armadillo repeat containing, X-linked 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 494468
Homologene: 128366
Mtss2
Name: MTSS I-BAR domain containing 2
Synonyms: ABBA, Mtss1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244654
Homologene: 70926
Phf11b
Name: PHD finger protein 11B
Synonyms: Gm4902
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 236451
Homologene: 87807
1500011B03Rik
Name: RIKEN cDNA 1500011B03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66236
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 164,072,116 bp
  • T to C, chromosome 2 at 30,408,217 bp
  • T to C, chromosome 2 at 69,513,285 bp
  • T to C, chromosome 2 at 135,335,493 bp
  • G to T, chromosome 3 at 28,124,702 bp
  • A to G, chromosome 3 at 106,482,411 bp
  • A to C, chromosome 4 at 133,695,699 bp
  • A to T, chromosome 5 at 21,290,571 bp
  • A to T, chromosome 5 at 91,027,562 bp
  • T to C, chromosome 5 at 114,813,193 bp
  • T to C, chromosome 5 at 120,529,598 bp
  • T to C, chromosome 5 at 142,529,178 bp
  • A to G, chromosome 6 at 29,287,706 bp
  • A to C, chromosome 6 at 47,577,685 bp
  • C to A, chromosome 6 at 115,792,211 bp
  • A to T, chromosome 7 at 20,883,592 bp
  • T to C, chromosome 7 at 23,320,979 bp
  • T to C, chromosome 7 at 78,905,941 bp
  • T to C, chromosome 7 at 110,852,360 bp
  • T to C, chromosome 8 at 3,611,015 bp
  • A to G, chromosome 8 at 110,738,505 bp
  • T to C, chromosome 8 at 111,990,280 bp
  • T to A, chromosome 9 at 64,288,103 bp
  • C to A, chromosome 11 at 53,578,918 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to G, chromosome 11 at 89,301,422 bp
  • T to C, chromosome 11 at 101,457,891 bp
  • G to A, chromosome 13 at 55,412,061 bp
  • G to A, chromosome 13 at 61,197,593 bp
  • T to C, chromosome 14 at 30,900,953 bp
  • G to A, chromosome 14 at 59,341,285 bp
  • A to G, chromosome 16 at 48,310,514 bp
  • A to G, chromosome 16 at 97,552,005 bp
  • T to C, chromosome 19 at 29,380,448 bp
  • T to C, chromosome 19 at 54,046,735 bp
  • T to A, chromosome X at 135,746,507 bp
  • A to G, chromosome X at 152,211,281 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4553 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041595-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.