Strain Name:
C57BL/6J-MtgxR4556Btlr/Mmmh
Stock Number:
041597-MU
Citation ID:
RRID:MMRRC_041597-MU
Other Names:
R4556 (G1), C57BL/6J-MtgxR4556Btlr
Major Collection:

Strain Information

Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, OTTMUSG00000012483, C030040A15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: an, 2900018K03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Fbxo42
Name: F-box protein 42
Synonyms: 6720460I06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213499
Homologene: 16234
Pros1
Name: protein S (alpha)
Synonyms: protein S
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19128
HGNC: HGNC:9456
Homologene: 264
Erc2
Name: ELKS/RAB6-interacting/CAST family member 2
Synonyms: D14Ertd171e, CAST, ELKS2alpha
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 238988
Homologene: 69188
Xrcc4
Name: X-ray repair complementing defective repair in Chinese hamster cells 4
Synonyms: 2310057B22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 108138
Homologene: 2555
Rmnd5b
Name: required for meiotic nuclear division 5 homolog B
Synonyms: 0610039K22Rik, Gid2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66089
Homologene: 100777
Sh2d3c
Name: SH2 domain containing 3C
Synonyms: SH2-containing Eph receptor-binding protein 1, Nsp3, Shep1, Chat, Cas/HEF1-associated signal transducer
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27387
Homologene: 69145
Gdf5
Name: growth differentiation factor 5
Synonyms: cartilage-derived morphogenetic protein-1, CDMP-1, brp, bp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14563
HGNC: HGNC:4220
Homologene: 468
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: Nav1.4, SkM1, mH2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 110880
Homologene: 283
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Stab2
Name: stabilin 2
Synonyms: FEEL-2, STAB-2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 192188
Homologene: 23022
A830005F24Rik
Name: RIKEN cDNA A830005F24 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 442803
Nr1h5
Name: nuclear receptor subfamily 1, group H, member 5
Synonyms: FXRB
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 381463
Homologene: 131277
Or1e29
Name: olfactory receptor family 1 subfamily E member 29
Synonyms: MOR135-6, Olfr389, GA_x6K02T2P1NL-3932085-3931147
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259011
Homologene: 85925
Thap12
Name: THAP domain containing 12
Synonyms: Dap4, Prkrir, 2900052B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72981
HGNC: HGNC:9440
Homologene: 37952
Gucy2e
Name: guanylate cyclase 2e
Synonyms: GC1, ROS-GC1, GC-E
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14919
HGNC: HGNC:4689
Homologene: 55442
Vmn1r27
Name: vomeronasal 1 receptor 27
Synonyms: V1rc33
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171206
Homologene: 79462
Coil
Name: coilin
Synonyms: p80-coilin, Cln80
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12812
HGNC: HGNC:2184
Homologene: 3413
Sh3tc1
Name: SH3 domain and tetratricopeptide repeats 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231147
Homologene: 10360
Adamts17
Name: ADAM metallopeptidase with thrombospondin type 1 motif 17
Synonyms: AU023434
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233332
Homologene: 16373
Or7e174
Name: olfactory receptor family 7 subfamily E member 174
Synonyms: Olfr868, MOR145-4, GA_x6K02T2PVTD-13841888-13842802
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258552
HGNC: HGNC:8396
Homologene: 74168
Napsa
Name: napsin A aspartic peptidase
Synonyms: NAP1, napsin, Kdap, pronapsin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16541
Homologene: 68418
Tas2r102
Name: taste receptor, type 2, member 102
Synonyms: STC 9-7, Tas2r2, mGR02, mt2r51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387339
Homologene: 41543
Ndufaf7
Name: NADH:ubiquinone oxidoreductase complex assembly factor 7
Synonyms: 2410091C18Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73694
VEGA: 17
Homologene: 12508
Lxn
Name: latexin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17035
Homologene: 36361
Tmem225
Name: transmembrane protein 225
Synonyms: 1700030E15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75667
VEGA: 9
Homologene: 52854
Mcub
Name: modifier of curly bare
Synonyms: 9030408N13Rik, Ccdc109b
Type: Gene
Species: Mouse
Chromosome: 3
Vmn1r229
Name: vomeronasal 1 receptor 229
Synonyms: V1re1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171224
Homologene: 74319
Rnf25
Name: ring finger protein 25
Synonyms: 0610009H16Rik, AO7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 57751
Homologene: 11193
Fbxo4
Name: F-box protein 4
Synonyms: 1700096C12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106052
Homologene: 8134
Ptprv
Name: protein tyrosine phosphatase receptor type V
Synonyms: Esp, OST, mOST-PTP, OST-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Gm20488
Name: predicted gene 20488
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Hoxb3os
Name: homeobox B3 and homeobox B2, opposite strand
Synonyms: Gm11530
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 102632302
Smim22
Name: small integral membrane protein 22
Synonyms: Gm5480
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 432995
Homologene: 133207
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 74,599,105 bp
  • A to T, chromosome 1 at 135,119,291 bp
  • A to G, chromosome 2 at 32,753,009 bp
  • G to A, chromosome 2 at 49,279,394 bp
  • A to G, chromosome 2 at 155,941,862 bp
  • T to A, chromosome 3 at 67,458,620 bp
  • G to A, chromosome 3 at 102,946,141 bp
  • G to A, chromosome 3 at 129,915,735 bp
  • A to G, chromosome 4 at 70,239,312 bp
  • T to A, chromosome 4 at 141,199,010 bp
  • T to C, chromosome 5 at 35,707,082 bp
  • T to C, chromosome 5 at 149,427,472 bp
  • A to G, chromosome 6 at 58,215,819 bp
  • T to C, chromosome 6 at 132,762,915 bp
  • A to C, chromosome 7 at 44,582,564 bp
  • A to T, chromosome 7 at 67,027,893 bp
  • A to G, chromosome 7 at 98,715,845 bp
  • T to C, chromosome 9 at 20,101,323 bp
  • T to C, chromosome 9 at 40,149,466 bp
  • T to G, chromosome 10 at 86,967,679 bp
  • G to T, chromosome 11 at 51,626,905 bp
  • T to C, chromosome 11 at 69,232,827 bp
  • G to A, chromosome 11 at 73,776,481 bp
  • A to G, chromosome 11 at 88,987,951 bp
  • C to A, chromosome 11 at 96,353,371 bp
  • T to C, chromosome 11 at 106,320,446 bp
  • T to C, chromosome 13 at 48,514,461 bp
  • A to T, chromosome 13 at 89,992,504 bp
  • A to C, chromosome 14 at 28,302,904 bp
  • A to G, chromosome 15 at 3,965,705 bp
  • T to A, chromosome 16 at 5,007,866 bp
  • A to G, chromosome 16 at 62,900,673 bp
  • T to A, chromosome 17 at 20,814,691 bp
  • T to C, chromosome 17 at 78,942,087 bp
  • G to T, chromosome 18 at 12,479,759 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4556 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041597-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.