Strain Name:
C57BL/6J-MtgxR4002Btlr/Mmmh
Stock Number:
041609-MU
Citation ID:
RRID:MMRRC_041609-MU
Other Names:
R4002 (G1), C57BL/6J-MtgxR4002Btlr
Major Collection:

Gene Information

2610021A01Rik
Name: RIKEN cDNA 2610021A01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668572
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Dmxl2
Name: Dmx-like 2
Synonyms: E130119P06Rik, 6430411K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Dbx1
Name: developing brain homeobox 1
Synonyms: Mmox C, Dbx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13172
Homologene: 19002
Tcp1
Name: t-complex protein 1
Synonyms: Ccta, c-cpn, TRic, CCT, Tcp-1, Tp63, p63, Cct1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21454
Homologene: 5656
Ttf2
Name: transcription termination factor, RNA polymerase II
Synonyms: 4632434F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74044
Homologene: 37826
Kcna4
Name: potassium voltage-gated channel, shaker-related subfamily, member 4
Synonyms: Kv1.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16492
HGNC: HGNC:6222
Homologene: 20514
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: LTBP-1, 9430031G15Rik, b2b1000Clo, Ltbp1L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Serpinb6d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6d
Synonyms: SPI3D, Gm11390
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238568
HGNC: HGNC:8950
Tlr11
Name: toll-like receptor 11
Synonyms: LOC239081
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239081
Homologene: 77905
Keg1
Name: kidney expressed gene 1
Synonyms: GS4059, 0610008P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 64697
Homologene: 69461
Zbbx
Name: zinc finger, B-box domain containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213234
Homologene: 11661
Olfr853
Name: olfactory receptor 853
Synonyms: GA_x6K02T2PVTD-13277703-13276786, MOR154-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258908
HGNC: HGNC:8466
Homologene: 133623
Obsl1
Name: obscurin-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98733
Prkcsh
Name: protein kinase C substrate 80K-H
Synonyms: 80K-H, hepatocystin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19089
VEGA: 9
HGNC: HGNC:9411
Homologene: 2056
Cdh18
Name: cadherin 18
Synonyms: B230220E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320865
HGNC: HGNC:1757
Homologene: 55858
Tcf19
Name: transcription factor 19
Synonyms: 5730403J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106795
Homologene: 5156
BC005624
Name: cDNA sequence BC005624
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227707
Homologene: 9551
Olfr855
Name: olfactory receptor 855
Synonyms: GA_x6K02T2PVTD-13330461-13331399, MOR148-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258517
Homologene: 74176
Asah1
Name: N-acylsphingosine amidohydrolase 1
Synonyms: acid ceramidase, 2310081N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11886
HGNC: HGNC:735
Homologene: 10504
Ces3a
Name: carboxylesterase 3A
Synonyms: Es-male carboxylesterase, Es31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382053
HGNC: HGNC:1865
Homologene: 84407
Olfr129
Name: olfactory receptor 129
Synonyms: MOR263-9, GA_x6K02T2PSCP-2503741-2502776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258324
Homologene: 133641
Gm5434
Name: predicted gene 5434
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 432649
VEGA: 12
Zfp810
Name: zinc finger protein 810
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235050
VEGA: 9
Homologene: 138299
Grcc10
Name: gene rich cluster, C10 gene
Synonyms: 2310033H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14790
Homologene: 8402
Higd2a
Name: HIG1 domain family, member 2A
Synonyms: 2010110M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67044
Homologene: 32648
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 53,631,681 bp
  • G to A, chromosome 1 at 75,500,099 bp
  • C to G, chromosome 2 at 30,978,886 bp
  • C to T, chromosome 2 at 107,295,914 bp
  • C to T, chromosome 3 at 75,105,671 bp
  • G to A, chromosome 3 at 100,948,225 bp
  • A to T, chromosome 6 at 124,740,970 bp
  • T to A, chromosome 7 at 41,625,540 bp
  • T to C, chromosome 7 at 49,636,517 bp
  • A to G, chromosome 8 at 41,348,139 bp
  • A to T, chromosome 8 at 105,057,461 bp
  • C to T, chromosome 9 at 19,537,906 bp
  • T to G, chromosome 9 at 19,584,714 bp
  • T to C, chromosome 9 at 22,010,615 bp
  • C to T, chromosome 9 at 22,278,892 bp
  • A to G, chromosome 9 at 54,473,832 bp
  • A to T, chromosome 11 at 9,585,415 bp
  • G to A, chromosome 12 at 36,090,636 bp
  • A to G, chromosome 13 at 33,670,647 bp
  • G to C, chromosome 13 at 54,590,727 bp
  • GA to GAA, chromosome 13 at 81,540,132 bp
  • T to C, chromosome 14 at 50,362,527 bp
  • T to A, chromosome 15 at 23,382,962 bp
  • T to C, chromosome 17 at 12,920,471 bp
  • T to C, chromosome 17 at 13,883,917 bp
  • A to G, chromosome 17 at 35,515,925 bp
  • G to A, chromosome 17 at 38,054,988 bp
  • T to C, chromosome 17 at 75,310,159 bp
  • T to A, chromosome 19 at 12,718,943 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4002 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041609-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.