Strain Name:
C57BL/6J-MtgxR4212Btlr/Mmmh
Stock Number:
041641-MU
Citation ID:
RRID:MMRRC_041641-MU
Other Names:
R4212 (G1), C57BL/6J-MtgxR4212Btlr
Major Collection:

Gene Information

Cd4
Name: CD4 antigen
Synonyms: L3T4, Ly-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12504
HGNC: HGNC:1678
Homologene: 513
Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74244
Homologene: 4662
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Chrnb2
Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Synonyms: [b]2-nAchR, Acrb-2, Acrb2, C030030P04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20347
Homologene: 20972
Uck2
Name: uridine-cytidine kinase 2
Synonyms: TSA903, Umpk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 80914
Homologene: 40850
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20273
Homologene: 7927
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, SAP90/PSD-95-associated protein 4, PSD-95/SAP90 binding protein 4, DAP4, Sapap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228836
Homologene: 8935
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93742
Homologene: 10489
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Polr1c
Name: polymerase (RNA) I polypeptide C
Synonyms: RPA40, 40kDa, Rpo1-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20016
Homologene: 3586
Pvt1
Name: Pvt1 oncogene
Synonyms: Mis-1, Pvt-1, Mlvi-1, Ayu21-84Imeg, Gt(Ayu21)84Imeg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19296
HGNC: HGNC:9709
Ppp2r5e
Name: protein phosphatase 2, regulatory subunit B', epsilon
Synonyms: protein phosphatase 2A subunit beta, 4633401M22Rik, B56beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26932
VEGA: 12
HGNC: HGNC:9313
Homologene: 55962
Psmd12
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Synonyms: P55, 1500002F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66997
HGNC: HGNC:9557
Homologene: 2109
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, TFIIIB90, TAF3B1, TFC5, Tfnr, G630013P12Rik, B130055N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230249
Homologene: 6056
Sfxn5
Name: sideroflexin 5
Synonyms: C230001H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94282
Homologene: 16936
Islr2
Name: immunoglobulin superfamily containing leucine-rich repeat 2
Synonyms: B930052A04Rik, mbu-3, Linx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320563
VEGA: 9
Homologene: 10833
Atg4c
Name: autophagy related 4C, cysteine peptidase
Synonyms: autophagin 3, Apg4-C, Autl1, Apg4c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242557
Homologene: 56296
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, Rasl2-8, lipopolysaccharide response
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21898
Homologene: 41317
Robo3
Name: roundabout guidance receptor 3
Synonyms: Rig-1, Rbig1, Robo3b, Robo3a, Rig1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19649
Homologene: 32119
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320661
Homologene: 19716
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Jag1
Name: jagged 1
Synonyms: Serrate-1, Headturner, Htu, ABE2, Ozz, Gsfabe2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Itgae
Name: integrin alpha E, epithelial-associated
Synonyms: CD103, alpha-E1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16407
HGNC: HGNC:6147
Homologene: 113560
Nlrc4
Name: NLR family, CARD domain containing 4
Synonyms: 9530011P19Rik, Card12, Ipaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268973
VEGA: 17
Homologene: 10924
Sorcs1
Name: sortilin-related VPS10 domain containing receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 58178
Homologene: 10967
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4932408N08Rik, 4931407K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77627
Homologene: 11259
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
A1bg
Name: alpha-1-B glycoprotein
Synonyms: C44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 117586
VEGA: 15
HGNC: HGNC:5
Homologene: 11167
Man2c1
Name: mannosidase, alpha, class 2C, member 1
Synonyms: 1110025H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 73744
HGNC: HGNC:6827
Homologene: 4887
Olfr173
Name: olfactory receptor 173
Synonyms: GA_x54KRFPKG5P-54960233-54959268, MOR184-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259002
HGNC: HGNC:8349
Homologene: 45084
Tshz3
Name: teashirt zinc finger family member 3
Synonyms: A630038G13Rik, Zfp537, teashirt3, Tsh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243931
Homologene: 10835
Naip1
Name: NLR family, apoptosis inhibitory protein 1
Synonyms: D13Lsd1, Naip, Birc1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17940
VEGA: 13
HGNC: HGNC:7634
Homologene: 113589
2210016F16Rik
Name: RIKEN cDNA 2210016F16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 70153
Homologene: 13005
F830045P16Rik
Name: RIKEN cDNA F830045P16 gene
Synonyms: Sirpb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228592
Homologene: 136180
Stk-ps2
Name: serine/threonine kinase 2
Synonyms: Gm4776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212225
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll4, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: EG235580, 1110001D15Rik, Dvwa, Vwa6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68553
Homologene: 130754
Gucy2e
Name: guanylate cyclase 2e
Synonyms: ROS-GC1, GC-E, GC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14919
HGNC: HGNC:4689
Homologene: 55442
Adamts15
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235130
VEGA: 9
Homologene: 8610
Chrm3
Name: cholinergic receptor, muscarinic 3, cardiac
Synonyms: M3R, Chrm-3, muscarinic acetylcholine receptor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 12671
HGNC: HGNC:1952
Homologene: 20191
Hip1r
Name: huntingtin interacting protein 1 related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29816
Homologene: 78348
Myorg
Name: myogenesis regulating glycosidase (putative)
Synonyms: NET37, AI464131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329828
Homologene: 19853
Plch1
Name: phospholipase C, eta 1
Synonyms: PLCeta1, Plcl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269437
Homologene: 88833
Gm8765
Name: predicted gene 8765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 667693
Homologene: 134512
Arhgef39
Name: Rho guanine nucleotide exchange factor (GEF) 39
Synonyms: E130306D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230098
Homologene: 19225
Lrfn5
Name: leucine rich repeat and fibronectin type III domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238205
Homologene: 17582
Usp44
Name: ubiquitin specific peptidase 44
Synonyms: E430004F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327799
VEGA: 10
Homologene: 12961
Slc2a12
Name: solute carrier family 2 (facilitated glucose transporter), member 12
Synonyms: GLUT-12, Glut12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 353169
Homologene: 59263
Olfr1454
Name: olfactory receptor 1454
Synonyms: GA_x6K02T2RE5P-3390804-3391727, MOR202-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258687
Homologene: 120159
Gm648
Name: predicted gene 648
Synonyms: LOC270599
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 270599
Lats2
Name: large tumor suppressor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50523
HGNC: HGNC:6515
Homologene: 56678
Gc
Name: vitamin D binding protein
Synonyms: DBP, vitamin D binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14473
HGNC: HGNC:4187
Homologene: 486
Gm11559
Name: predicted gene 11559
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100415785
Homologene: 136354
Olfr723
Name: olfactory receptor 723
Synonyms: GA_x6K02T2PMLR-5600424-5599495, MOR247-3P, MOR247-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 259147
Homologene: 71986
Krtap17-1
Name: keratin associated protein 17-1
Synonyms: A030006P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77914
Pcdha7
Name: protocadherin alpha 7
Synonyms: Cnr4, Crnr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12939
HGNC: HGNC:8673
Homologene: 117962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 46,029,380 bp
  • C to T, chromosome 1 at 167,237,586 bp
  • A to G, chromosome 2 at 125,620,001 bp
  • C to T, chromosome 2 at 129,460,353 bp
  • T to C, chromosome 2 at 137,085,070 bp
  • A to G, chromosome 2 at 156,746,237 bp
  • T to A, chromosome 3 at 63,870,759 bp
  • A to T, chromosome 3 at 89,761,544 bp
  • T to C, chromosome 4 at 41,498,307 bp
  • T to C, chromosome 4 at 43,496,660 bp
  • T to C, chromosome 4 at 58,834,076 bp
  • T to A, chromosome 4 at 66,840,326 bp
  • T to C, chromosome 4 at 99,221,487 bp
  • A to G, chromosome 5 at 25,347,359 bp
  • A to T, chromosome 5 at 36,614,479 bp
  • T to C, chromosome 5 at 89,435,575 bp
  • A to G, chromosome 5 at 123,999,890 bp
  • A to T, chromosome 6 at 85,332,306 bp
  • G to A, chromosome 6 at 114,703,425 bp
  • A to G, chromosome 6 at 124,870,459 bp
  • A to G, chromosome 7 at 36,770,119 bp
  • T to A, chromosome 8 at 127,610,458 bp
  • A to G, chromosome 9 at 30,906,174 bp
  • C to T, chromosome 9 at 37,421,898 bp
  • T to C, chromosome 9 at 57,139,932 bp
  • C to T, chromosome 9 at 58,199,320 bp
  • C to T, chromosome 9 at 59,906,066 bp
  • T to A, chromosome 9 at 106,075,370 bp
  • C to T, chromosome 9 at 107,603,398 bp
  • A to T, chromosome 10 at 22,702,094 bp
  • A to G, chromosome 10 at 93,846,770 bp
  • A to G, chromosome 11 at 69,228,123 bp
  • A to G, chromosome 11 at 73,119,352 bp
  • T to A, chromosome 11 at 79,469,798 bp
  • A to G, chromosome 11 at 99,864,900 bp
  • A to G, chromosome 11 at 99,993,914 bp
  • T to C, chromosome 11 at 107,485,759 bp
  • A to G, chromosome 12 at 55,739,330 bp
  • A to T, chromosome 12 at 61,843,820 bp
  • A to G, chromosome 12 at 75,469,551 bp
  • T to C, chromosome 13 at 9,877,755 bp
  • C to T, chromosome 13 at 50,700,352 bp
  • C to T, chromosome 13 at 58,381,991 bp
  • T to A, chromosome 13 at 100,059,585 bp
  • T to C, chromosome 13 at 100,426,875 bp
  • A to T, chromosome 14 at 49,928,889 bp
  • C to T, chromosome 14 at 57,696,255 bp
  • G to T, chromosome 15 at 60,919,736 bp
  • C to T, chromosome 15 at 62,178,235 bp
  • T to C, chromosome 15 at 83,892,863 bp
  • A to G, chromosome 15 at 98,845,003 bp
  • T to C, chromosome 15 at 100,957,073 bp
  • T to A, chromosome 16 at 58,797,369 bp
  • A to G, chromosome 17 at 46,246,120 bp
  • T to C, chromosome 17 at 74,447,115 bp
  • A to G, chromosome 18 at 36,974,974 bp
  • A to T, chromosome 18 at 60,916,701 bp
  • T to G, chromosome 19 at 13,063,759 bp
  • G to A, chromosome 19 at 50,225,175 bp
  • C to T, chromosome X at 56,545,208 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4212 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041641-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.