Strain Name:
C57BL/6J-MtgxR4275Btlr/Mmmh
Stock Number:
041646-MU
Citation ID:
RRID:MMRRC_041646-MU
Other Names:
R4275 (G1), C57BL/6J-MtgxR4275Btlr
Major Collection:

Gene Information

Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: 2610028O16Rik, D11Ertd530e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 52615
Homologene: 32256
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: SMemb, nonmuscle myosin heavy chain IIB, Fltn, NMHC II-B, NMHC-B, Myosin IIB, nonmuscle myosin heavy chain II-B, Fltn, 9330167F11Rik, Myhn2, 5730504C04Rik, Myhn-2, myosin IIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 208628
Homologene: 32227
Usp19
Name: ubiquitin specific peptidase 19
Synonyms: 8430421I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 71472
Homologene: 41730
Camsap2
Name: calmodulin regulated spectrin-associated protein family, member 2
Synonyms: 4930541M15Rik, Camsap1l1, 1600013L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67886
Homologene: 18927
Smg6
Name: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103677
Homologene: 23024
Hdac2
Name: histone deacetylase 2
Synonyms: Yy1bp, D10Wsu179e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15182
HGNC: HGNC:4853
Homologene: 68187
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67610
Homologene: 12164
Fbxl5
Name: F-box and leucine-rich repeat protein 5
Synonyms: Fir4, Fbl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 242960
Homologene: 8129
Myadm
Name: myeloid-associated differentiation marker
Synonyms: D7Wsu62e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 50918
HGNC: HGNC:7544
Homologene: 9668
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Pcn, Plc, perlecan, per
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Scpep1
Name: serine carboxypeptidase 1
Synonyms: 2410018F01Rik, Risc, 4833411K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74617
Homologene: 41443
Nadk
Name: NAD kinase
Synonyms: 4432404C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 192185
Homologene: 49724
Tmem139
Name: transmembrane protein 139
Synonyms: A930027H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 109218
VEGA: 6
Homologene: 51920
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Enpep
Name: glutamyl aminopeptidase
Synonyms: Ly51, APA, Bp-1/6C3, 6030431M22Rik, aminopeptidase-A, Ly-51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mgr1, Mass1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 110789
Homologene: 19815
Fam131b
Name: family with sequence similarity 131, member B
Synonyms: 6530406I18Rik, 6330503C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 76156
Homologene: 8797
Vipr1
Name: vasoactive intestinal peptide receptor 1
Synonyms: VIP receptor subtype 1, VIP-R1, VPAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22354
Homologene: 3399
Htra1
Name: HtrA serine peptidase 1
Synonyms: insulin-like growth factor binding protein 5 protease, RSPP11, L56, HtrA1, Prss11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56213
HGNC: HGNC:9476
Homologene: 31114
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 81877
Homologene: 49589
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627743
Homologene: 129680
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Papolg
Name: poly(A) polymerase gamma
Synonyms: 9630006B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216578
Homologene: 56959
Zfp518b
Name: zinc finger protein 518B
Synonyms: 6820424L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100515
Homologene: 19115
Serpina3m
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3M
Synonyms: Spi2.4, contrapsin-like, MMSPi2.4, Spi2-rs1, antitrypsin, alpha-1 antiproteinase, Spi-2l, 3e46, MMCM7, Spi-2rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20717
HGNC: HGNC:16
Homologene: 111129
Zfp651
Name: zinc finger protein 651
Synonyms: 4732420M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270210
VEGA: 9
Homologene: 136302
Mettl21c
Name: methyltransferase like 21C
Synonyms: A530098C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 433294
Homologene: 45916
D3Ertd751e
Name: DNA segment, Chr 3, ERATO Doi 751, expressed
Synonyms: 4930415G15Rik, 2810009O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 73852
Homologene: 12520
Daxx
Name: Fas death domain-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13163
HGNC: HGNC:2681
Homologene: 1033
Dhcr7
Name: 7-dehydrocholesterol reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13360
HGNC: HGNC:2860
Homologene: 1042
Mrgprh
Name: MAS-related GPR, member H
Synonyms: MrgH, Gpr90
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 80978
VEGA: 17
Homologene: 12758
Tbc1d25
Name: TBC1 domain family, member 25
Synonyms: DXHXS7927e, mMg81, 6330576A08Rik, Oatl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 209815
HGNC: HGNC:8092
Homologene: 16173
Fam126b
Name: family with sequence similarity 126, member B
Synonyms: D1Ertd53e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 213056
Homologene: 18184
Mapk8ip2
Name: mitogen-activated protein kinase 8 interacting protein 2
Synonyms: JNK-interacting protein, IB2, 3230402N03Rik, Jip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 60597
HGNC: HGNC:6883
Homologene: 8201
Olfr996
Name: olfactory receptor 996
Synonyms: MOR175-4, GA_x6K02T2Q125-47058060-47059004
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258429
Homologene: 85934
Sall2
Name: spalt like transcription factor 2
Synonyms: Msal-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50524
Homologene: 9269
Olfr1089
Name: olfactory receptor 1089
Synonyms: GA_x6K02T2Q125-48226321-48225386, MOR193-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 257933
Homologene: 79354
Igf2
Name: insulin-like growth factor 2
Synonyms: Igf-2, M6pr, Mpr, Peg2, Igf-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16002
HGNC: HGNC:5466
Homologene: 510
Rnase1
Name: ribonuclease, RNase A family, 1 (pancreatic)
Synonyms: Rib-1, Rib1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 19752
VEGA: 14
Homologene: 7919
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,058,384 bp
  • C to T, chromosome 1 at 44,010,556 bp
  • T to C, chromosome 1 at 58,529,933 bp
  • A to G, chromosome 1 at 136,270,876 bp
  • T to C, chromosome 1 at 154,493,325 bp
  • T to C, chromosome 2 at 85,579,863 bp
  • T to C, chromosome 2 at 86,733,592 bp
  • C to A, chromosome 3 at 41,756,154 bp
  • A to T, chromosome 3 at 129,332,278 bp
  • C to T, chromosome 4 at 137,518,940 bp
  • T to A, chromosome 4 at 155,584,255 bp
  • A to G, chromosome 5 at 38,671,728 bp
  • T to A, chromosome 5 at 43,762,772 bp
  • T to A, chromosome 5 at 123,767,779 bp
  • A to G, chromosome 6 at 42,264,105 bp
  • G to A, chromosome 6 at 42,321,307 bp
  • A to G, chromosome 7 at 3,297,102 bp
  • T to C, chromosome 7 at 130,979,474 bp
  • T to C, chromosome 7 at 142,655,786 bp
  • C to T, chromosome 7 at 143,843,227 bp
  • G to T, chromosome 8 at 94,649,761 bp
  • T to G, chromosome 9 at 108,498,694 bp
  • T to C, chromosome 9 at 121,664,618 bp
  • T to A, chromosome 9 at 121,766,539 bp
  • A to G, chromosome 10 at 36,993,628 bp
  • A to G, chromosome 11 at 23,868,378 bp
  • A to T, chromosome 11 at 68,751,940 bp
  • A to C, chromosome 11 at 74,993,874 bp
  • T to C, chromosome 11 at 80,030,053 bp
  • T to C, chromosome 11 at 88,947,142 bp
  • T to A, chromosome 12 at 104,389,116 bp
  • A to T, chromosome 13 at 81,155,269 bp
  • A to T, chromosome 14 at 51,145,870 bp
  • C to A, chromosome 14 at 52,313,803 bp
  • T to C, chromosome 15 at 89,458,995 bp
  • T to C, chromosome 17 at 12,877,227 bp
  • T to A, chromosome 17 at 20,228,640 bp
  • A to G, chromosome 17 at 33,910,527 bp
  • A to G, chromosome 17 at 34,698,231 bp
  • C to G, chromosome X at 8,154,747 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4275 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041646-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.