Strain Name:
C57BL/6J-MtgxR4276Btlr/Mmmh
Stock Number:
041647-MU
Citation ID:
RRID:MMRRC_041647-MU
Other Names:
R4276 (G1), C57BL/6J-MtgxR4276Btlr
Major Collection:

Gene Information

Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Chrnb3
Name: cholinergic receptor, nicotinic, beta polypeptide 3
Synonyms: Acrb3, 5730417K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108043
HGNC: HGNC:1963
Homologene: 36035
Csnk1e
Name: casein kinase 1, epsilon
Synonyms: CKI epsilon, tau, KC1epsilon, CKIepsilon, CK1epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 27373
HGNC: HGNC:2453
Homologene: 121695
Brix1
Name: BRX1, biogenesis of ribosomes
Synonyms: 1110064N10Rik, Bxdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67832
Homologene: 10133
Pitpnb
Name: phosphatidylinositol transfer protein, beta
Synonyms: Pitp beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56305
HGNC: HGNC:9002
Homologene: 4533
Prrc2c
Name: proline-rich coiled-coil 2C
Synonyms: 9630039I18Rik, 1810043M20Rik, Bat2d, Bat2l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226562
Homologene: 41015
Arl6ip1
Name: ADP-ribosylation factor-like 6 interacting protein 1
Synonyms: AIP-6, ARMER
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54208
HGNC: HGNC:697
Homologene: 41008
Cfap36
Name: cilia and flagella associated protein 36
Synonyms: 4931428D14Rik, Ccdc104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216618
Homologene: 41646
Pus10
Name: pseudouridylate synthase 10
Synonyms: 2810013G11Rik, 4933435A13Rik, Ccdc139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74467
Homologene: 12566
Casp12
Name: caspase 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12364
Homologene: 7654
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Ern1
Name: endoplasmic reticulum (ER) to nucleus signalling 1
Synonyms: 9030414B18Rik, Ire1p, Ire1alpha, Ire1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VPF, VEGF-A, Vegf, VEGF120, VEGF188, VEGF164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22339
Homologene: 2534
Gpc6
Name: glypican 6
Synonyms: 6720429C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 23888
HGNC: HGNC:4454
Homologene: 55922
Znrf2
Name: zinc and ring finger 2
Synonyms: 1190002C14Rik, D6Ertd365e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387524
Homologene: 15668
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Insm1
Name: insulinoma-associated 1
Synonyms: IA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 53626
HGNC: HGNC:6090
Homologene: 31080
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nav2.3, NaG, Nav2, 1110034K09Rik, Scn6a, Nax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20272
Homologene: 55706
Jmjd8
Name: jumonji domain containing 8
Synonyms: 2610003J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72106
Homologene: 12441
Vmn2r93
Name: vomeronasal 2, receptor 93
Synonyms: EG627132
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627132
Homologene: 129750
Mroh1
Name: maestro heat-like repeat family member 1
Synonyms: D330001F17Rik, Heatr7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223658
Homologene: 44882
Spag16
Name: sperm associated antigen 16
Synonyms: Pf20, 4930524F24Rik, 4930585K05Rik, Wdr29, 4921511D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66722
Homologene: 11572
Proc
Name: protein C
Synonyms: inactivator of coagulation factors Va, VIII, PC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 19123
VEGA: 18
HGNC: HGNC:9451
Homologene: 37288
Padi2
Name: peptidyl arginine deiminase, type II
Synonyms: PAD type II, Pdi, Pdi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18600
Homologene: 7214
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218989
Homologene: 49506
Kcna5
Name: potassium voltage-gated channel, shaker-related subfamily, member 5
Synonyms: Kv1.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16493
HGNC: HGNC:6224
Homologene: 1683
Mefv
Name: Mediterranean fever
Synonyms: marenostrin, pyrin, FMF, TRIM20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 54483
VEGA: 16
HGNC: HGNC:6998
Homologene: 32441
Stmn4
Name: stathmin-like 4
Synonyms: RB3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56471
Homologene: 10496
Spata13
Name: spermatogenesis associated 13
Synonyms: ESTM11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219140
Homologene: 19482
Rabl2
Name: RAB, member RAS oncogene family-like 2
Synonyms: 1110031N17Rik, Rabl2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68708
Homologene: 90913
Gpcpd1
Name: glycerophosphocholine phosphodiesterase 1
Synonyms: 2310032D16Rik, 2310004G06Rik, Prei4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74182
Homologene: 10478
Gm6578
Name: predicted gene 6578
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 625347
Kbtbd8
Name: kelch repeat and BTB (POZ) domain containing 8
Synonyms: Takrp, SSEC-51, SSEC 51, SSEC51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243574
Homologene: 13043
Nt5c1b
Name: 5'-nucleotidase, cytosolic IB
Synonyms: 4921514H13Rik, CN-IB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70881
Homologene: 12370
Olfr1289
Name: olfactory receptor 1289
Synonyms: GA_x6K02T2Q125-72534883-72535821, MOR245-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258399
HGNC: HGNC:8301
Gimap8
Name: GTPase, IMAP family member 8
Synonyms: LOC243374, IAN9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243374
Homologene: 69703
Rtl6
Name: retrotransposon Gag like 6
Synonyms: Mart6, Mar6, Ldoc1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223732
VEGA: 15
Homologene: 18594
Acad8
Name: acyl-Coenzyme A dehydrogenase family, member 8
Synonyms: 2310016C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66948
HGNC: HGNC:87
Homologene: 8662
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Kctd6
Name: potassium channel tetramerisation domain containing 6
Synonyms: 5430433B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71393
VEGA: 14
Homologene: 12392
Lbx1
Name: ladybird homeobox 1
Synonyms: Lbx1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16814
Homologene: 4784
Pstpip2
Name: proline-serine-threonine phosphatase-interacting protein 2
Synonyms: cmo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 19201
VEGA: 18
HGNC: HGNC:9581
Homologene: 69150
Syp
Name: synaptophysin
Synonyms: Syp I, p38, Syn, A230093K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20977
Homologene: 2391
Olfr1038-ps
Name: olfactory receptor 1038, pseudogene
Synonyms: GA_x6K02T2Q125-47591072-47592031, MOR185-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259015
Homologene: 17463
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 69,873,481 bp
  • T to A, chromosome 1 at 162,673,591 bp
  • T to G, chromosome 2 at 66,684,063 bp
  • C to T, chromosome 2 at 69,938,387 bp
  • G to A, chromosome 2 at 86,122,279 bp
  • G to T, chromosome 2 at 111,483,504 bp
  • T to C, chromosome 2 at 132,540,287 bp
  • C to A, chromosome 2 at 146,222,968 bp
  • A to G, chromosome 4 at 140,936,548 bp
  • A to G, chromosome 5 at 111,371,392 bp
  • G to A, chromosome 6 at 12,100,188 bp
  • T to C, chromosome 6 at 32,200,948 bp
  • T to C, chromosome 6 at 48,659,083 bp
  • A to G, chromosome 6 at 54,863,901 bp
  • T to C, chromosome 6 at 95,126,933 bp
  • T to C, chromosome 6 at 126,533,366 bp
  • AAAATAAATAAATAAATAAATAAATA to AAAATAAATAAATAAATAAATAAATAAATA, chromosome 7 at 118,121,899 bp
  • A to G, chromosome 8 at 27,393,751 bp
  • T to C, chromosome 9 at 5,352,246 bp
  • C to A, chromosome 9 at 26,978,449 bp
  • A to G, chromosome 11 at 23,706,895 bp
  • A to G, chromosome 11 at 29,230,584 bp
  • T to C, chromosome 11 at 106,407,181 bp
  • A to G, chromosome 12 at 10,374,886 bp
  • G to C, chromosome 14 at 8,222,806 bp
  • G to A, chromosome 14 at 33,958,650 bp
  • C to T, chromosome 14 at 48,477,636 bp
  • C to T, chromosome 14 at 60,756,296 bp
  • A to T, chromosome 14 at 66,355,717 bp
  • A to G, chromosome 14 at 117,435,916 bp
  • T to C, chromosome 15 at 10,481,747 bp
  • T to G, chromosome 15 at 76,393,851 bp
  • T to C, chromosome 15 at 79,429,767 bp
  • A to G, chromosome 15 at 80,901,971 bp
  • T to A, chromosome 15 at 84,557,196 bp
  • G to A, chromosome 15 at 89,584,188 bp
  • A to T, chromosome 16 at 3,715,569 bp
  • T to C, chromosome 17 at 18,304,830 bp
  • A to G, chromosome 17 at 25,829,813 bp
  • A to G, chromosome 17 at 46,031,466 bp
  • A to G, chromosome 18 at 32,135,914 bp
  • T to C, chromosome 18 at 37,012,090 bp
  • A to G, chromosome 18 at 77,861,856 bp
  • C to A, chromosome 19 at 45,235,089 bp
  • G to T, chromosome X at 7,638,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4276 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041647-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.