Strain Name:
C57BL/6J-MtgxR4281Btlr/Mmmh
Stock Number:
041649-MU
Citation ID:
RRID:MMRRC_041649-MU
Other Names:
R4281 (G1), C57BL/6J-MtgxR4281Btlr
Major Collection:

Strain Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Arl6ip1
Name: ADP-ribosylation factor-like 6 interacting protein 1
Synonyms: AIP-6, ARMER
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54208
HGNC: HGNC:697
Homologene: 41008
Mark4
Name: MAP/microtubule affinity regulating kinase 4
Synonyms: Markl1, 2410090P21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232944
Homologene: 57146
Rars1
Name: arginyl-tRNA synthetase 1
Synonyms: 2610037E21Rik, 2610011N19Rik, Rars
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104458
HGNC: HGNC:9870
Homologene: 68281
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 404710
Homologene: 26400
Snx29
Name: sorting nexin 29
Synonyms: LOC381035, LOC385605, 4933437K13Rik, Gm11170, Rundc2a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74478
Homologene: 32706
Rasa3
Name: RAS p21 protein activator 3
Synonyms: GAPIII activator 3, Ras GTPase-activating protein III, GAPIII, R-Ras gap, scat, hlb381
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19414
Homologene: 7217
Hepacam2
Name: HEPACAM family member 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101202
Homologene: 18724
Pnpla6
Name: patatin-like phospholipase domain containing 6
Synonyms: Swiss-cheese, MSws, Nte
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50767
Homologene: 21333
Trim33
Name: tripartite motif-containing 33
Synonyms: Tif1g, 8030451N04Rik, ectodermin, Ecto
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 94093
Homologene: 9296
Evc2
Name: EvC ciliary complex subunit 2
Synonyms: limbin, Lbn, Ellis van Creveld syndrome 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68525
Homologene: 17117
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210274
Homologene: 105965
Pcdh18
Name: protocadherin 18
Synonyms: PCDH68L
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73173
Homologene: 10389
Npepl1
Name: aminopeptidase-like 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228961
Homologene: 11647
Dnaaf11
Name: dynein axonemal assembly factor 11
Synonyms: LRTP, Lrrc6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 54562
Homologene: 34534
Gpr39
Name: G protein-coupled receptor 39
Synonyms: 4933415E13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71111
HGNC: HGNC:4496
Homologene: 20380
Gmip
Name: Gem-interacting protein
Synonyms: 5031419I10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78816
Homologene: 9570
Slco6b1
Name: solute carrier organic anion transporter family, member 6b1
Synonyms: 1700022M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67854
Homologene: 12199
Rimbp2
Name: RIMS binding protein 2
Synonyms: A930033C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231760
Homologene: 14672
Or11h7
Name: olfactory receptor family 11 subfamily H member 7
Synonyms: GA_x6K02T2PMLR-6372116-6373060, MOR106-12, Olfr746
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258295
Homologene: 134081
Zfp692
Name: zinc finger protein 692
Synonyms: Zfp692-ps
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103836
Homologene: 9889
Sned1
Name: sushi, nidogen and EGF-like domains 1
Synonyms: Snep, 6720455I24Rik, D430044C15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208777
Homologene: 14708
Zfp28
Name: zinc finger protein 28
Synonyms: mkr-5, Zfp-28, 2810438M17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22690
Homologene: 69047
Zbtb24
Name: zinc finger and BTB domain containing 24
Synonyms: ZNF450
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268294
VEGA: 10
Homologene: 8870
Tmem132a
Name: transmembrane protein 132A
Synonyms: 6720481D13Rik, Hspa5bp1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 98170
Homologene: 75076
Or12j3
Name: olfactory receptor family 12 subfamily J member 3
Synonyms: GA_x6K02T2PBJ9-42523824-42522901, MOR252-2, Olfr530
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258512
Homologene: 128384
Fam210b
Name: family with sequence similarity 210, member B
Synonyms: 2010011I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67017
Homologene: 32646
B9d2
Name: B9 protein domain 2
Synonyms: stumpy
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232987
Homologene: 12733
Gtf2a1l
Name: general transcription factor IIA, 1-like
Synonyms: 1700011N16Rik, Alf, Gtf2a1lf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71828
Homologene: 86732
Gimap8
Name: GTPase, IMAP family member 8
Synonyms: LOC243374, IAN9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243374
Homologene: 69703
Tmem229b-ps
Name: transmembrane protein 229B, pseudogene
Synonyms: Gm5423
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432460
VEGA: 10
Zfp612
Name: zinc finger protein 612
Synonyms: B230354B21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234725
Homologene: 27083
Ppp4r3c1
Name: protein phosphatase 4 regulatory subunit 3C1
Synonyms: 4930415L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245511
Homologene: 133109
Magea14
Name: MAGE family member A14
Synonyms: 1700080O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 74279
Defa27
Name: defensin, alpha, 27
Synonyms: ENSMUSG00000074438, Gm15299
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100041811
Homologene: 113382
Pnma5
Name: paraneoplastic antigen family 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 385377
Homologene: 14176
Cavin3
Name: caveolae associated 3
Synonyms: 3110015B12Rik, 6330514M23Rik, cavin 3, Prkcdbp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 109042
HGNC: HGNC:9400
Homologene: 12490
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 93,285,855 bp
  • A to G, chromosome 1 at 96,997,390 bp
  • G to A, chromosome 1 at 125,677,991 bp
  • A to C, chromosome 1 at 127,963,877 bp
  • A to G, chromosome 2 at 35,298,871 bp
  • T to C, chromosome 2 at 172,351,548 bp
  • T to C, chromosome 2 at 174,116,091 bp
  • A to G, chromosome 3 at 49,756,533 bp
  • T to C, chromosome 3 at 88,098,860 bp
  • T to A, chromosome 3 at 103,329,086 bp
  • T to C, chromosome 5 at 37,338,594 bp
  • C to T, chromosome 5 at 128,788,340 bp
  • G to A, chromosome 6 at 3,475,938 bp
  • A to C, chromosome 6 at 48,658,820 bp
  • A to T, chromosome 7 at 6,393,701 bp
  • T to C, chromosome 7 at 19,433,446 bp
  • A to G, chromosome 7 at 25,681,426 bp
  • A to G, chromosome 7 at 87,297,524 bp
  • A to C, chromosome 7 at 105,481,774 bp
  • A to T, chromosome 7 at 112,109,993 bp
  • AAAATAAATAAATAAATAAATAAATA to AAAATAAATAAATAAATAAATAAATAAATA, chromosome 7 at 118,121,899 bp
  • A to G, chromosome 7 at 140,373,472 bp
  • C to T, chromosome 7 at 144,395,480 bp
  • T to C, chromosome 8 at 3,521,513 bp
  • A to G, chromosome 8 at 13,588,946 bp
  • A to G, chromosome 8 at 18,978,077 bp
  • A to G, chromosome 8 at 21,315,616 bp
  • T to A, chromosome 8 at 69,813,601 bp
  • A to G, chromosome 8 at 110,090,059 bp
  • T to C, chromosome 10 at 41,464,920 bp
  • A to G, chromosome 10 at 53,475,378 bp
  • T to C, chromosome 11 at 35,821,224 bp
  • T to C, chromosome 11 at 58,314,265 bp
  • G to A, chromosome 12 at 3,901,665 bp
  • T to C, chromosome 14 at 50,653,572 bp
  • A to T, chromosome 15 at 66,380,529 bp
  • C to T, chromosome 16 at 11,631,493 bp
  • A to G, chromosome 16 at 15,806,099 bp
  • A to G, chromosome 17 at 88,711,637 bp
  • C to G, chromosome 19 at 10,861,726 bp
  • T to C, chromosome X at 51,968,990 bp
  • T to C, chromosome X at 73,035,430 bp
  • A to T, chromosome X at 89,932,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4281 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041649-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.