Strain Name:
C57BL/6J-MtgxR4307Btlr/Mmmh
Stock Number:
041658-MU
Citation ID:
RRID:MMRRC_041658-MU
Other Names:
R4307 (G1), C57BL/6J-MtgxR4307Btlr
Major Collection:

Gene Information

Egf
Name: epidermal growth factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 13645
HGNC: HGNC:3229
Homologene: 1483
Inpp5a
Name: inositol polyphosphate-5-phosphatase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 212111
HGNC: HGNC:6076
Homologene: 4045
Gpm6a
Name: glycoprotein m6a
Synonyms: Gpm6, M6A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234267
HGNC: HGNC:4460
Homologene: 3864
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216860
Homologene: 13029
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Top3b
Name: topoisomerase (DNA) III beta
Synonyms: Topo III beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 21976
Homologene: 2923
Gpbp1
Name: GC-rich promoter binding protein 1
Synonyms: 1700034P14Rik, D230035M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 73274
Homologene: 11292
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Mrtfa
Name: myocardin related transcription factor A
Synonyms: Bsac, Mal, Mkl1, MRTF-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223701
Homologene: 32487
Efemp2
Name: epidermal growth factor-containing fibulin-like extracellular matrix protein 2
Synonyms: MBP1, Fbln4, fibulin-4, fibulin 4, 0610011K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 58859
HGNC: HGNC:3219
Homologene: 32339
1500009L16Rik
Name: RIKEN cDNA 1500009L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 69784
VEGA: 10
Homologene: 136237
H2-K1
Name: histocompatibility 2, K1, K region
Synonyms: H-2K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14972
HGNC: HGNC:4931
Homologene: 128352
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 75050
VEGA: 13
Homologene: 69235
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nav2.3, 1110034K09Rik, Nax, Scn6a, Nav2, NaG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20272
Homologene: 55706
Fcsk
Name: fucose kinase
Synonyms: Fuk, L-fucose kinase, 1110046B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234730
Homologene: 15452
Ccr5
Name: chemokine (C-C motif) receptor 5
Synonyms: CD195, Cmkbr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12774
HGNC: HGNC:1606
Homologene: 37325
Unc13c
Name: unc-13 homolog C
Synonyms: Munc13-3, 1500037O19Rik, D9Ertd414e, Unc13h3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208898
Homologene: 45443
Frmd4a
Name: FERM domain containing 4A
Synonyms: 2700017I06Rik, C230040M21Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 209630
Homologene: 9971
Ankrd24
Name: ankyrin repeat domain 24
Synonyms: 5730519E19Rik, 4631433D01Rik, D10Bur2e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70615
Homologene: 49885
Asap2
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms: LOC385250, 6530401G17Rik, Ddef2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 211914
HGNC: HGNC:2721
Homologene: 2888
Vmn2r28
Name: vomeronasal 2, receptor 28
Synonyms: EG665255
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 665255
HGNC: null
Homologene: 129605
A830018L16Rik
Name: RIKEN cDNA A830018L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 320492
Homologene: 14194
Vmn2r101
Name: vomeronasal 2, receptor 101
Synonyms: EG627576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627576
HGNC: null
Homologene: 115024
Emilin3
Name: elastin microfibril interfacer 3
Synonyms: EMILIN-T, Emilin5, 1110013O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 280635
Homologene: 18817
Itgb5
Name: integrin beta 5
Synonyms: [b]5B, [b]-5, [b]5A, [b]5, ESTM23, beta-5, beta5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 16419
HGNC: HGNC:6160
Homologene: 20511
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 627569
HGNC: null
Homologene: 129606
Lca5l
Name: Leber congenital amaurosis 5-like
Synonyms: 4921526F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 385668
HGNC: HGNC:1255
Homologene: 47978
Slc5a11
Name: solute carrier family 5 (sodium/glucose cotransporter), member 11
Synonyms: Kst1, 2010013B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233836
Homologene: 14184
Prr27
Name: proline rich 27
Synonyms: 4930432K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 73779
Homologene: 136277
Cfap157
Name: cilia and flagella associated protein 157
Synonyms: 1700019L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227736
Homologene: 53056
Gm20939
Name: predicted gene, 20939
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 100044193
VEGA: 17
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 11,972,076 bp
  • A to G, chromosome 1 at 63,572,931 bp
  • C to T, chromosome 2 at 4,333,078 bp
  • G to A, chromosome 2 at 32,779,042 bp
  • A to T, chromosome 2 at 66,675,755 bp
  • T to C, chromosome 2 at 160,908,317 bp
  • T to A, chromosome 3 at 129,719,095 bp
  • A to G, chromosome 5 at 87,842,907 bp
  • A to G, chromosome 5 at 109,086,006 bp
  • A to G, chromosome 6 at 32,163,509 bp
  • A to T, chromosome 7 at 5,490,708 bp
  • T to A, chromosome 7 at 123,269,870 bp
  • T to C, chromosome 7 at 139,574,963 bp
  • T to C, chromosome 8 at 55,047,393 bp
  • G to A, chromosome 8 at 110,892,080 bp
  • T to C, chromosome 9 at 73,693,367 bp
  • T to C, chromosome 9 at 124,125,074 bp
  • T to C, chromosome 10 at 81,646,331 bp
  • A to C, chromosome 10 at 83,737,928 bp
  • T to C, chromosome 11 at 69,907,948 bp
  • T to C, chromosome 12 at 21,229,481 bp
  • C to T, chromosome 13 at 58,344,123 bp
  • C to T, chromosome 13 at 96,496,666 bp
  • T to C, chromosome 13 at 111,448,983 bp
  • G to A, chromosome 15 at 81,016,347 bp
  • T to C, chromosome 16 at 16,889,617 bp
  • T to G, chromosome 16 at 33,948,732 bp
  • C to T, chromosome 16 at 96,159,556 bp
  • T to A, chromosome 17 at 19,590,161 bp
  • C to T, chromosome 17 at 33,999,816 bp
  • A to T, chromosome 17 at 94,876,734 bp
  • T to A, chromosome 19 at 5,481,621 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4307 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041658-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.