Strain Name:
C57BL/6J-MtgxR4308Btlr/Mmmh
Stock Number:
041659-MU
Citation ID:
RRID:MMRRC_041659-MU
Other Names:
R4308 (G1), C57BL/6J-MtgxR4308Btlr
Major Collection:

Gene Information

Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Lamb1
Name: laminin B1
Synonyms: C80098, Lamb1-1, C81607, Lamb-1, D130003D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Cyp39a1
Name: cytochrome P450, family 39, subfamily a, polypeptide 1
Synonyms: oxysterol 7-alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56050
VEGA: 17
Homologene: 9580
Kif15
Name: kinesin family member 15
Synonyms: 3930402I10Rik, 3110023M17Rik, HKLP2, N-10 kinesin, Knsl7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Ascc1
Name: activating signal cointegrator 1 complex subunit 1
Synonyms: 1810015P09Rik, CGI-18, ASC1p50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69090
VEGA: 10
Homologene: 41079
Rpl7a
Name: ribosomal protein L7A
Synonyms: Surf-3, surfeit 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27176
Homologene: 105462
Myo1b
Name: myosin IB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17912
HGNC: HGNC:7596
Homologene: 7856
L3mbtl3
Name: L3MBTL3 histone methyl-lysine binding protein
Synonyms: MBT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237339
Homologene: 18226
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665775
Homologene: 45109
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: LOC381562, A930005E13Rik, 1810009A16Rik, p600, D930005K06Rik, Zubr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Npc1
Name: NPC intracellular cholesterol transporter 1
Synonyms: C85354, lcsd, D18Ertd723e, nmf164, D18Ertd139e, A430089E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18145
HGNC: HGNC:7897
Homologene: 228
Dars2
Name: aspartyl-tRNA synthetase 2 (mitochondrial)
Synonyms: 5830468K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226539
Homologene: 7093
Sft2d2
Name: SFT2 domain containing 2
Synonyms: cI-45, 2010005O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108735
Homologene: 27069
Obi1
Name: ORC ubiquitin ligase 1
Synonyms: 2810449K13Rik, Rnf219, 2610206B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72486
VEGA: 14
Homologene: 11578
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: C730036B14Rik, D12Ertd364e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: mt2r50, Tas2r24, T2R24, mGR24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387351
Homologene: 87293
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mass1, VLGR1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242627
Homologene: 135888
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: 1810073P09Rik, X83337, ESTM15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75607
Homologene: 19155
Arhgef5
Name: Rho guanine nucleotide exchange factor (GEF) 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54324
Homologene: 66300
Olfr116
Name: olfactory receptor 116
Synonyms: GA_x6K02T2PSCP-2084102-2083137, MOR218-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258625
Myo5b
Name: myosin VB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17919
HGNC: HGNC:7603
Homologene: 49481
Vmn2r18
Name: vomeronasal 2, receptor 18
Synonyms: EG632671
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 632671
Mical2
Name: microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms: 5330438E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320878
Homologene: 8760
Asap2
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms: Ddef2, 6530401G17Rik, LOC385250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211914
HGNC: HGNC:2721
Homologene: 2888
Scaf11
Name: SR-related CTD-associated factor 11
Synonyms: Srsf2ip, SRRP129, 1110061H03Rik, SIP1, Sfrs2ip, CASP11, 2610510E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72193
VEGA: 15
Homologene: 37957
Pcdhb1
Name: protocadherin beta 1
Synonyms: PcdhbA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93872
HGNC: HGNC:8680
Homologene: 8348
Fktn
Name: fukutin
Synonyms: Fcmd, Fukutin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 246179
HGNC: HGNC:3622
Homologene: 31402
Zfp983
Name: zinc finger protein 983
Synonyms: 3110052M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73229
VEGA: 17
Rbm20
Name: RNA binding motif protein 20
Synonyms: 1110018J23Rik, 2010003H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73713
Homologene: 28386
Iqca
Name: IQ motif containing with AAA domain
Synonyms: 4930585L22Rik, 4930465P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74918
Homologene: 49784
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627569
Homologene: 129606
Rnf144b
Name: ring finger protein 144B
Synonyms: Ibrdc2, E130105P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218215
VEGA: 13
Homologene: 27092
Gsdmcl1
Name: gasdermin C-like 1
Synonyms: Gsdmdc2, 1700022L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74236
Prr27
Name: proline rich 27
Synonyms: 4930432K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73779
Homologene: 136277
Cfap157
Name: cilia and flagella associated protein 157
Synonyms: 1700019L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227736
Homologene: 53056
Gpr33
Name: G protein-coupled receptor 33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 14762
HGNC: HGNC:4489
Homologene: 7345
Rwdd2b
Name: RWD domain containing 2B
Synonyms: ORF5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 53858
HGNC: HGNC:1302
Homologene: 9654
Olfr1148
Name: olfactory receptor 1148
Synonyms: GA_x6K02T2Q125-49334566-49335510, MOR264-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258220
Homologene: 79465
Ivd
Name: isovaleryl coenzyme A dehydrogenase
Synonyms: 6720455E18Rik, 1300016K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56357
HGNC: HGNC:6186
Homologene: 1676
RP24-388P13.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Il31
Name: interleukin 31
Synonyms: 1700013B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76399
Homologene: 130770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 51,883,109 bp
  • A to G, chromosome 1 at 64,736,574 bp
  • T to C, chromosome 1 at 90,144,897 bp
  • A to G, chromosome 1 at 161,041,721 bp
  • A to G, chromosome 1 at 165,188,264 bp
  • T to C, chromosome 2 at 26,913,266 bp
  • G to A, chromosome 2 at 32,779,042 bp
  • T to C, chromosome 2 at 87,833,270 bp
  • T to A, chromosome 2 at 118,877,886 bp
  • C to T, chromosome 4 at 53,724,617 bp
  • A to T, chromosome 4 at 113,483,967 bp
  • T to C, chromosome 4 at 139,472,509 bp
  • A to T, chromosome 5 at 41,791,813 bp
  • A to G, chromosome 5 at 87,842,907 bp
  • A to G, chromosome 5 at 109,086,006 bp
  • A to G, chromosome 5 at 123,480,706 bp
  • A to T, chromosome 5 at 151,584,803 bp
  • C to T, chromosome 6 at 43,279,498 bp
  • A to G, chromosome 6 at 132,754,991 bp
  • C to A, chromosome 7 at 112,331,992 bp
  • C to G, chromosome 9 at 63,235,304 bp
  • T to G, chromosome 9 at 123,013,982 bp
  • C to T, chromosome 10 at 26,282,792 bp
  • T to G, chromosome 10 at 60,013,612 bp
  • T to C, chromosome 12 at 21,229,481 bp
  • T to C, chromosome 12 at 31,329,255 bp
  • A to T, chromosome 12 at 52,023,640 bp
  • A to T, chromosome 12 at 65,126,531 bp
  • A to C, chromosome 13 at 3,569,498 bp
  • A to G, chromosome 13 at 47,242,942 bp
  • C to T, chromosome 13 at 49,090,837 bp
  • G to A, chromosome 13 at 81,440,192 bp
  • T to A, chromosome 14 at 104,479,593 bp
  • A to G, chromosome 15 at 63,848,705 bp
  • A to G, chromosome 15 at 96,446,515 bp
  • A to T, chromosome 16 at 87,436,727 bp
  • A to G, chromosome 17 at 21,662,208 bp
  • C to T, chromosome 17 at 37,623,736 bp
  • T to A, chromosome 17 at 43,730,964 bp
  • C to T, chromosome 18 at 12,210,527 bp
  • A to T, chromosome 18 at 37,266,661 bp
  • A to G, chromosome 18 at 74,731,740 bp
  • A to G, chromosome 19 at 53,843,260 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4308 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041659-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.