Strain Name:
C57BL/6J-MtgxR4399Btlr/Mmmh
Stock Number:
041686-MU
Citation ID:
RRID:MMRRC_041686-MU
Other Names:
R4399 (G1), C57BL/6J-MtgxR4399Btlr
Major Collection:

Gene Information

Thrap3
Name: thyroid hormone receptor associated protein 3
Synonyms: Trap150, 9330151F09Rik, B230333E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230753
Homologene: 31289
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: Tulip1, 2310003F20Rik, 4930400K19Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Cramp1l
Name: cramped chromatin regulator homolog 1
Synonyms: Tce4, 5830477H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57354
VEGA: 17
Homologene: 35373
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: Dnahc7, LOC381341, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Ibsp
Name: integrin binding sialoprotein
Synonyms: Bsp, bone sialoprotein, Bsp2, BSP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15891
HGNC: HGNC:5341
Homologene: 3644
St8sia5
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Synonyms: Siat8e, ST8SiaV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225742
Homologene: 8331
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 5430439G14Rik, 6330406J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71389
Homologene: 32772
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Sh2d3c
Name: SH2 domain containing 3C
Synonyms: Nsp3, Shep1, SH2-containing Eph receptor-binding protein 1, Chat, Cas/HEF1-associated signal transducer
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27387
Homologene: 69145
Setd1b
Name: SET domain containing 1B
Synonyms: KMT2G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208043
Homologene: 134654
Olfr59
Name: olfactory receptor 59
Synonyms: MOR133-3P, GA_x6K02T2P1NL-4434429-4435400, IH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18359
Homologene: 73924
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20192
Homologene: 68151
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171263
Homologene: 128344
Abcc6
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
Synonyms: Mrp6, DCC, Dyscalc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27421
HGNC: HGNC:57
Homologene: 55559
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66282
Homologene: 10146
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Col6a5, Gm7455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 665033
Homologene: 122792
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: EG244114, Vmn2r72-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244114
Homologene: 115466
Olfr347
Name: olfactory receptor 347
Synonyms: MOR136-9, GA_x6K02T2NLDC-33428755-33429693
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258945
Homologene: 51790
Tctn2
Name: tectonic family member 2
Synonyms: 4432405B04Rik, Tect2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67978
Homologene: 11729
Slc2a7
Name: solute carrier family 2 (facilitated glucose transporter), member 7
Synonyms: OTTMUSG00000010396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435818
Homologene: 72470
Cnga1
Name: cyclic nucleotide gated channel alpha 1
Synonyms: Cncg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12788
HGNC: HGNC:2148
Homologene: 55432
Mrc2
Name: mannose receptor, C type 2
Synonyms: novel lectin, uPARAP, Endo180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Foxred2
Name: FAD-dependent oxidoreductase domain containing 2
Synonyms: LOC239554, D15Bwg0759e, A430097D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239554
Homologene: 11800
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Olfr706
Name: olfactory receptor 706
Synonyms: MOR283-11, GA_x6K02T2PBJ9-9271198-9270248
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258350
Homologene: 133646
Prkcz
Name: protein kinase C, zeta
Synonyms: zetaPKC, Pkcz, aPKCzeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18762
HGNC: HGNC:9412
Homologene: 55681
Tmem151a
Name: transmembrane protein 151A
Synonyms: Tmem151, LOC381199
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381199
Homologene: 17721
Sstr3
Name: somatostatin receptor 3
Synonyms: Smstr3, Smstr-3, sst3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20607
VEGA: 15
Homologene: 20285
G6pd2
Name: glucose-6-phosphate dehydrogenase 2
Synonyms: Gpd-2, G6pdx-ps1, Gpd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14380
Vmn2r50
Name: vomeronasal 2, receptor 50
Synonyms: EG434117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434117
Homologene: 113703
Sult1c1
Name: sulfotransferase family, cytosolic, 1C, member 1
Synonyms: Stp2, mOLFST, P-SULT, Sult1a2, (PST)G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20888
Homologene: 56817
Olfr1286
Name: olfactory receptor 1286
Synonyms: MOR248-21, GA_x6K02T2Q125-72472405-72471488
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277562
Homologene: 73992
Slc35b3
Name: solute carrier family 35, member B3
Synonyms: PAPST2, 4921526O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 108652
VEGA: 13
Homologene: 9313
Sbpl
Name: spermine binding protein-like
Synonyms: 2310068J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 638345
VEGA: 17
Homologene: 117464
Aga
Name: aspartylglucosaminidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11593
HGNC: HGNC:318
Homologene: 13
Mup18
Name: major urinary protein 18
Synonyms: Gm12561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100048884
Homologene: 74304
Prrg3
Name: proline rich Gla (G-carboxyglutamic acid) 3 (transmembrane)
Synonyms: LOC208748
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 208748
Homologene: 11452
Igkv6-25
Name: immunoglobulin kappa chain variable 6-25
Synonyms: Gm16943
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381784
Gm22204
Name: predicted gene, 22204
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,518,727 bp
  • G to A, chromosome 2 at 32,746,160 bp
  • A to T, chromosome 2 at 36,735,230 bp
  • T to G, chromosome 2 at 111,420,799 bp
  • A to T, chromosome 2 at 112,946,844 bp
  • G to A, chromosome 2 at 125,587,980 bp
  • T to C, chromosome 2 at 160,965,318 bp
  • C to T, chromosome 4 at 61,672,629 bp
  • A to G, chromosome 4 at 126,167,079 bp
  • A to G, chromosome 4 at 150,158,550 bp
  • A to T, chromosome 4 at 155,269,077 bp
  • T to A, chromosome 5 at 61,810,173 bp
  • T to C, chromosome 5 at 72,604,381 bp
  • T to A, chromosome 5 at 104,309,282 bp
  • C to T, chromosome 5 at 123,161,798 bp
  • T to C, chromosome 5 at 124,602,063 bp
  • T to A, chromosome 6 at 70,215,710 bp
  • A to G, chromosome 7 at 10,047,907 bp
  • A to G, chromosome 7 at 10,447,549 bp
  • T to C, chromosome 7 at 46,002,607 bp
  • TCC to TC, chromosome 7 at 85,221,550 bp
  • T to C, chromosome 7 at 85,738,500 bp
  • G to T, chromosome 7 at 106,886,453 bp
  • C to A, chromosome 8 at 3,614,244 bp
  • T to C, chromosome 8 at 53,511,826 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • T to A, chromosome 9 at 105,888,965 bp
  • A to G, chromosome 11 at 74,288,856 bp
  • T to A, chromosome 11 at 105,336,658 bp
  • T to C, chromosome 11 at 109,936,385 bp
  • T to A, chromosome 12 at 55,795,778 bp
  • A to G, chromosome 13 at 38,937,815 bp
  • A to C, chromosome 15 at 77,953,358 bp
  • T to C, chromosome 15 at 77,955,680 bp
  • T to G, chromosome 15 at 78,540,124 bp
  • A to G, chromosome 17 at 23,954,886 bp
  • C to T, chromosome 17 at 24,979,585 bp
  • T to C, chromosome 17 at 53,962,510 bp
  • T to C, chromosome 18 at 77,253,018 bp
  • A to G, chromosome 19 at 5,083,071 bp
  • A to T, chromosome X at 71,967,309 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4399 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041686-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.