Strain Name:
C57BL/6J-MtgxR4408Btlr/Mmmh
Stock Number:
041690-MU
Citation ID:
RRID:MMRRC_041690-MU
Other Names:
R4408 (G1), C57BL/6J-MtgxR4408Btlr
Major Collection:

Gene Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Lrrn3
Name: leucine rich repeat protein 3, neuronal
Synonyms: NLRR-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16981
VEGA: 12
Homologene: 36315
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C530050H10Rik, C130067A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320713
Homologene: 66204
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 30940
VEGA: 16
Homologene: 8374
Gpbp1
Name: GC-rich promoter binding protein 1
Synonyms: 1700034P14Rik, D230035M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 73274
Homologene: 11292
Map3k12
Name: mitogen-activated protein kinase kinase kinase 12
Synonyms: Zpk, DLK, MUK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26404
HGNC: HGNC:6851
Homologene: 4592
Card6
Name: caspase recruitment domain family, member 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239319
Homologene: 13067
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Cspp1
Name: centrosome and spindle pole associated protein 1
Synonyms: 4930413O22Rik, 2310020J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211660
Homologene: 23487
Fbln1
Name: fibulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14114
HGNC: HGNC:3600
Homologene: 21295
Hnrnpu
Name: heterogeneous nuclear ribonucleoprotein U
Synonyms: scaffold attachment factor A, Sp120, Hnrpu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 51810
HGNC: HGNC:5048
Homologene: 22991
Myof
Name: myoferlin
Synonyms: 2310051D19Rik, E030042N20Rik, Fer1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226101
VEGA: 19
HGNC: HGNC:3656
Homologene: 40882
Vwa3a
Name: von Willebrand factor A domain containing 3A
Synonyms: E030013G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233813
Homologene: 18607
Gml2
Name: glycosylphosphatidylinositol anchored molecule like 2
Synonyms: HemT, hematopoietic cell-specific transcript, 1700057K19Rik, Hemt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15202
HGNC: HGNC:4375
Homologene: 48071
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75823
Homologene: 27384
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26564
Homologene: 55831
Olfr1283
Name: olfactory receptor 1283
Synonyms: GA_x6K02T2Q125-72420217-72421134, MOR248-19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228443
Homologene: 133657
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Irf5
Name: interferon regulatory factor 5
Synonyms: mirf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27056
HGNC: HGNC:6120
Homologene: 8088
Pop5
Name: processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae)
Synonyms: 1500019J17Rik, Rnasep3, 2700077E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 117109
Homologene: 41076
Vmn1r235
Name: vomeronasal 1 receptor 235
Synonyms: V1rf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171233
Homologene: 74360
Sgsm2
Name: small G protein signaling modulator 2
Synonyms: D630003G22Rik, Rutbc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 97761
Homologene: 14780
Olfr466
Name: olfactory receptor 466
Synonyms: GA_x6K02T2PB7A-3051266-3052192, MOR209-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 258816
Homologene: 72032
Vmn1r23
Name: vomeronasal 1 receptor 23
Synonyms: V1rc24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171197
Homologene: 110825
Slc16a11
Name: solute carrier family 16 (monocarboxylic acid transporters), member 11
Synonyms: MNCb-2717
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216867
Homologene: 27409
Fndc5
Name: fibronectin type III domain containing 5
Synonyms: PeP, 1500001L03Rik, Pxp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 384061
Homologene: 17812
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434172
Homologene: 45597
Vmn2r33
Name: vomeronasal 2, receptor 33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 624512
Homologene: 113703
Cbarp
Name: calcium channel, voltage-dependent, beta subunit associated regulatory protein
Synonyms: R29144/1, Dos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100503659
Homologene: 82309
Osr2
Name: odd-skipped related 2
Synonyms: 5430409I15Rik, Osr2B, Osr2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 107587
VEGA: 15
Homologene: 14200
Gm26919
Name: predicted gene, 26919
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Homologene: 134227
Gm10799
Name: predicted gene 10799
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Spag17os
Name: sperm associated antigen 17, opposite strand
Synonyms: B930086A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,058,775 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to C, chromosome 1 at 178,330,803 bp
  • T to A, chromosome 2 at 69,467,169 bp
  • C to A, chromosome 2 at 104,068,064 bp
  • T to A, chromosome 2 at 111,369,280 bp
  • T to A, chromosome 2 at 126,116,125 bp
  • T to A, chromosome 3 at 100,103,378 bp
  • T to C, chromosome 4 at 94,965,212 bp
  • A to G, chromosome 4 at 129,142,529 bp
  • C to T, chromosome 5 at 115,240,777 bp
  • A to G, chromosome 6 at 29,534,001 bp
  • T to C, chromosome 6 at 57,926,368 bp
  • A to T, chromosome 6 at 130,677,765 bp
  • A to C, chromosome 7 at 7,551,230 bp
  • A to G, chromosome 7 at 41,286,448 bp
  • G to A, chromosome 7 at 120,778,926 bp
  • T to C, chromosome 10 at 51,628,543 bp
  • G to A, chromosome 10 at 80,134,568 bp
  • A to G, chromosome 11 at 70,215,734 bp
  • C to A, chromosome 11 at 74,851,766 bp
  • A to G, chromosome 12 at 41,454,042 bp
  • A to G, chromosome 13 at 53,118,961 bp
  • A to G, chromosome 13 at 65,152,700 bp
  • A to T, chromosome 13 at 111,448,964 bp
  • T to A, chromosome 15 at 5,101,054 bp
  • T to C, chromosome 15 at 35,300,471 bp
  • C to T, chromosome 15 at 35,709,294 bp
  • T to C, chromosome 15 at 74,824,339 bp
  • T to A, chromosome 15 at 85,231,556 bp
  • T to C, chromosome 15 at 102,505,402 bp
  • A to C, chromosome 16 at 77,115,453 bp
  • A to G, chromosome 17 at 21,261,592 bp
  • A to G, chromosome 17 at 25,380,627 bp
  • A to G, chromosome 19 at 37,922,978 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041690-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.