Strain Name:
C57BL/6J-MtgxR4426Btlr/Mmmh
Stock Number:
041697-MU
Citation ID:
RRID:MMRRC_041697-MU
Other Names:
R4426 (G1), C57BL/6J-MtgxR4426Btlr
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Nms
Name: neuromedin S
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 433292
Homologene: 67775
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Nbea
Name: neurobeachin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Tmem87b
Name: transmembrane protein 87B
Synonyms: 2810431I02Rik, 2610301K12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72477
Homologene: 69513
Csnk1a1
Name: casein kinase 1, alpha 1
Synonyms: 4632404G05Rik, 2610208K14Rik, 5430427P18Rik, CK1a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93687
HGNC: HGNC:2451
Homologene: 111694
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216161
VEGA: 10
Homologene: 8981
Utp18
Name: UTP18 small subunit processome component
Synonyms: 6230425C22Rik, Wdr50
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217109
Homologene: 41087
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Gbf1
Name: golgi-specific brefeldin A-resistance factor 1
Synonyms: 1700083E03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107338
HGNC: HGNC:4181
Homologene: 37897
Slc44a1
Name: solute carrier family 44, member 1
Synonyms: CHTL1, CTL1, 4833416H08Rik, Cdw92, 2210409B22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100434
Homologene: 11137
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 84585
Homologene: 11112
Cic
Name: capicua transcriptional repressor
Synonyms: 1200010B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71722
Homologene: 87637
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268902
Homologene: 43188
Nrip1
Name: nuclear receptor interacting protein 1
Synonyms: RIP140, 6030458L20Rik, 8430438I05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268903
HGNC: HGNC:8001
Homologene: 2606
Gas2l1
Name: growth arrest-specific 2 like 1
Synonyms: TU-71.1, D0Jmb1, GAR22, 4930500E24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78926
Homologene: 4733
Vwc2
Name: von Willebrand factor C domain containing 2
Synonyms: A930041G11Rik, Brorin, cradin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319922
Homologene: 18542
Far1
Name: fatty acyl CoA reductase 1
Synonyms: 2600011M19Rik, 3732409C05Rik, Mlstd2, 2900034E22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67420
Homologene: 41718
Tpm1
Name: tropomyosin 1, alpha
Synonyms: Tpm-1, alpha-TM, TM2, Tm3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22003
Homologene: 128637
Polr2c
Name: polymerase (RNA) II (DNA directed) polypeptide C
Synonyms: mRBP31, 33kDa, Rpo2-3, RPB3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20021
HGNC: HGNC:9189
Homologene: 2017
Mpdz
Name: multiple PDZ domain crumbs cell polarity complex component
Synonyms: MUPP1, B930003D11Rik, multiple PDZ domain protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17475
HGNC: HGNC:7208
Homologene: 2841
Pitpnm1
Name: phosphatidylinositol transfer protein, membrane-associated 1
Synonyms: RdgB, DRES9
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18739
HGNC: HGNC:9003
Homologene: 3608
Trmt2a
Name: TRM2 tRNA methyltransferase 2A
Synonyms: Htf9c
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15547
Homologene: 7374
Hoxa13
Name: homeobox A13
Synonyms: Hox-1.10
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15398
HGNC: HGNC:5102
Homologene: 73882
Eif4e1b
Name: eukaryotic translation initiation factor 4E family member 1B
Synonyms: Eif4eloo
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218268
Homologene: 100945
Fut4
Name: fucosyltransferase 4
Synonyms: FAL, FucT-IV, 3-fucosyl-N-acetyl-lactosamine epitope, SSEA-1, LeX, Ssea1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14345
VEGA: 9
HGNC: HGNC:4015
Homologene: 1538
Rap1gap2
Name: RAP1 GTPase activating protein 2
Synonyms: LOC380710, Garnl4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380711
Homologene: 56695
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Nwd1
Name: NACHT and WD repeat domain containing 1
Synonyms: A230063L24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319555
Homologene: 72261
Vmn2r61
Name: vomeronasal 2, receptor 61
Synonyms: Casr-rs2, EG637873, Gprc2a-rs2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637873
Homologene: 129683
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Pitpnm2
Name: phosphatidylinositol transfer protein, membrane-associated 2
Synonyms: RDGBA2, NIR3, Rdgb2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19679
Homologene: 7915
Ppp1r9b
Name: protein phosphatase 1, regulatory subunit 9B
Synonyms: neurabin II, spinophilin, Spn, SPL
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217124
HGNC: HGNC:9298
Homologene: 32787
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: EG244114, Vmn2r72-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244114
Homologene: 115466
Tns1
Name: tensin 1
Synonyms: 1200014E20Rik, 1110018I21Rik, E030018G17Rik, Tns, E030037J05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21961
Homologene: 11219
Cacnb2
Name: calcium channel, voltage-dependent, beta 2 subunit
Synonyms: Cchb2, Cavbeta2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12296
HGNC: HGNC:1402
Homologene: 75191
Albfm1
Name: albumin superfamily member 1
Synonyms: ARG, Gm17754, 5830473C10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 622307
Homologene: 129803
Abca2
Name: ATP-binding cassette, sub-family A member 2
Synonyms: Abc2, D2H0S1474E
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11305
HGNC: HGNC:32
Homologene: 55590
Tm6sf2
Name: transmembrane 6 superfamily member 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 107770
Homologene: 77694
Vmn1r39
Name: vomeronasal 1 receptor 39
Synonyms: Gm5993
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 546903
Homologene: 110800
Or52ad1
Name: olfactory receptor family 52 subfamily AD member 1
Synonyms: GA_x6K02T2PBJ9-6056235-6055291, MOR39-1, Olfr600
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259048
Homologene: 17476
Atp8a1
Name: ATPase phospholipid transporting 8A1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11980
Homologene: 48402
Uevld
Name: UEV and lactate/malate dehyrogenase domains
Synonyms: 8430408E05Rik, Attp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54122
Homologene: 69251
Chil5
Name: chitinase-like 5
Synonyms: Bclp2, Bclp1, Chi3l7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229687
Homologene: 132668
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20392
Homologene: 31205
Pgm1
Name: phosphoglucomutase 1
Synonyms: Pgm-2, 2610020G18Rik, Pgm1a, Pgm2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72157
HGNC: HGNC:8905
Homologene: 1979
Ttll10
Name: tubulin tyrosine ligase-like family, member 10
Synonyms: 4930595O22Rik, 4833412E22Rik, Ttll5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 330010
Homologene: 51912
Zfp715
Name: zinc finger protein 715
Synonyms: 2610041B18Rik, mszf15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69930
Homologene: 74591
Arhgef39
Name: Rho guanine nucleotide exchange factor 39
Synonyms: E130306D19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230098
Homologene: 19225
Galnt7
Name: polypeptide N-acetylgalactosaminyltransferase 7
Synonyms: ppGaNTase-T7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108150
HGNC: HGNC:4129
Homologene: 9685
Vmn2r106
Name: vomeronasal 2, receptor 106
Synonyms: EG224576
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224576
Homologene: 135824
Edil3
Name: EGF-like repeats and discoidin I-like domains 3
Synonyms: Del1, developmental endothelial locus-1, Del-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13612
HGNC: HGNC:3173
Homologene: 21166
Shc3
Name: src homology 2 domain-containing transforming protein C3
Synonyms: ShcC, N-Shc, Rai
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20418
VEGA: 13
Homologene: 7536
Stx5a
Name: syntaxin 5A
Synonyms: syntaxin 5, 0610031F24Rik, D19Ertd627e
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56389
Homologene: 2381
Or51f1d
Name: olfactory receptor family 51 subfamily F member 1D
Synonyms: GA_x6K02T2PBJ9-5762668-5763618, MOR14-6, Olfr583
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258752
Homologene: 133721
Poc1b
Name: POC1 centriolar protein B
Synonyms: 4933430F16Rik, Wdr51b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 382406
Homologene: 41728
Ccdc137
Name: coiled-coil domain containing 137
Synonyms: 3110023B02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67291
Homologene: 34992
Nde1
Name: nudE neurodevelopment protein 1
Synonyms: 2810027M15Rik, mNudE
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67203
Homologene: 32354
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12041
Homologene: 37642
Krtap10-31
Name: keratin associated protein 10-31
Synonyms: EG622629, Gm10318
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 622629
Homologene: 115744
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Radx
Name: RPA1 related single stranded DNA binding protein, X-linked
Synonyms: D330045A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 102871
Homologene: 9962
Timd6
Name: T cell immunoglobulin and mucin domain containing 6
Synonyms: BC053393
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 407814
Homologene: 86792
Rnf166
Name: ring finger protein 166
Synonyms: 1110031E24Rik, Zfp313l
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68718
Homologene: 35335
Vmn1r207
Name: vomeronasal 1 receptor 207
Synonyms: Gm11312, Vmn1r207-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432735
Homologene: 110880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,347,924 bp
  • C to T, chromosome 1 at 38,939,296 bp
  • A to G, chromosome 1 at 73,985,749 bp
  • G to A, chromosome 1 at 185,235,342 bp
  • A to T, chromosome 2 at 14,975,215 bp
  • G to A, chromosome 2 at 25,446,565 bp
  • G to T, chromosome 2 at 69,506,348 bp
  • C to T, chromosome 2 at 128,846,750 bp
  • G to A, chromosome 3 at 56,082,379 bp
  • A to G, chromosome 3 at 87,976,042 bp
  • A to G, chromosome 3 at 106,019,627 bp
  • C to T, chromosome 4 at 43,497,112 bp
  • T to C, chromosome 4 at 53,563,286 bp
  • G to A, chromosome 4 at 81,286,426 bp
  • T to C, chromosome 4 at 99,962,140 bp
  • G to C, chromosome 4 at 156,048,561 bp
  • A to C, chromosome 5 at 67,774,828 bp
  • G to T, chromosome 5 at 90,572,783 bp
  • C to T, chromosome 5 at 124,142,123 bp
  • G to A, chromosome 6 at 4,691,459 bp
  • C to A, chromosome 6 at 52,260,729 bp
  • A to T, chromosome 6 at 66,805,361 bp
  • C to T, chromosome 7 at 25,294,008 bp
  • A to C, chromosome 7 at 27,423,798 bp
  • A to T, chromosome 7 at 42,300,733 bp
  • T to C, chromosome 7 at 42,300,735 bp
  • T to C, chromosome 7 at 43,311,092 bp
  • A to T, chromosome 7 at 46,940,142 bp
  • G to A, chromosome 7 at 85,737,828 bp
  • T to C, chromosome 7 at 103,051,811 bp
  • A to G, chromosome 7 at 103,346,083 bp
  • C to A, chromosome 7 at 113,550,001 bp
  • A to G, chromosome 7 at 127,905,333 bp
  • G to T, chromosome 8 at 57,552,572 bp
  • A to G, chromosome 8 at 70,077,394 bp
  • A to G, chromosome 8 at 72,666,795 bp
  • A to T, chromosome 8 at 94,863,462 bp
  • A to G, chromosome 8 at 122,470,240 bp
  • T to C, chromosome 9 at 14,751,381 bp
  • T to C, chromosome 9 at 66,496,005 bp
  • A to G, chromosome 9 at 67,032,565 bp
  • C to T, chromosome 9 at 108,070,247 bp
  • G to A, chromosome 10 at 27,422,558 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • T to A, chromosome 10 at 77,853,222 bp
  • T to C, chromosome 10 at 80,072,358 bp
  • T to C, chromosome 10 at 99,155,139 bp
  • A to G, chromosome 11 at 5,063,908 bp
  • A to G, chromosome 11 at 11,154,235 bp
  • T to A, chromosome 11 at 46,584,420 bp
  • C to A, chromosome 11 at 74,407,322 bp
  • T to C, chromosome 11 at 93,866,438 bp
  • A to T, chromosome 11 at 95,001,324 bp
  • T to C, chromosome 11 at 120,460,248 bp
  • T to A, chromosome 12 at 114,583,796 bp
  • T to C, chromosome 13 at 22,726,600 bp
  • C to G, chromosome 13 at 51,480,094 bp
  • T to C, chromosome 13 at 54,784,483 bp
  • T to A, chromosome 13 at 89,042,607 bp
  • A to G, chromosome 15 at 47,669,185 bp
  • A to G, chromosome 16 at 14,188,336 bp
  • A to G, chromosome 16 at 18,249,229 bp
  • T to A, chromosome 16 at 73,948,266 bp
  • T to C, chromosome 16 at 76,291,405 bp
  • A to G, chromosome 17 at 20,285,379 bp
  • T to C, chromosome 17 at 56,286,555 bp
  • C to T, chromosome 18 at 34,631,941 bp
  • T to A, chromosome 18 at 37,144,743 bp
  • A to G, chromosome 18 at 61,585,310 bp
  • T to C, chromosome 19 at 4,111,988 bp
  • A to G, chromosome 19 at 8,749,740 bp
  • T to G, chromosome 19 at 46,163,525 bp
  • T to C, chromosome X at 139,480,896 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4426 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041697-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.