Strain Name:
C57BL/6J-MtgxR4428Btlr/Mmmh
Stock Number:
041698-MU
Citation ID:
RRID:MMRRC_041698-MU
Other Names:
R4428 (G1), C57BL/6J-MtgxR4428Btlr
Major Collection:

Gene Information

Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: OTTMUSG00000012483, C030040A15Rik, 9430004D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 109241
Homologene: 81861
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: Acra-4, Acra4, a4 nicotinic receptor, alpha4 nAChR, alpha4-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 6030440P17Rik, ne, 8430406N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242022
Homologene: 18454
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: C030017F07Rik, Bravo, C130076O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Tert
Name: telomerase reverse transcriptase
Synonyms: TR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 21752
VEGA: 13
Homologene: 31141
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: C630029C19Rik, nur14, NaCh6, med, seal, motor end-plate disease, nmf2, mnd-2, mnd2, NMF335, ataxia 3, nmf335, Nav1.6, nmf58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 20273
Homologene: 7927
Gsk3b
Name: glycogen synthase kinase 3 beta
Synonyms: 7330414F15Rik, 8430431H08Rik, GSK-3, GSK3, GSK-3beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 56637
HGNC: HGNC:4617
Homologene: 55629
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 4932411G06Rik, 1700021E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 73254
Homologene: 35455
Skp2
Name: S-phase kinase-associated protein 2 (p45)
Synonyms: FBXL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 27401
Homologene: 55942
Kif18a
Name: kinesin family member 18A
Synonyms: gcd2, B130001M12Rik, N-8 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228421
Homologene: 41820
Umps
Name: uridine monophosphate synthetase
Synonyms: 1700095D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 22247
Homologene: 319
Tardbp
Name: TAR DNA binding protein
Synonyms: TDP-43, Tdp43, 1190002A23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230908
Homologene: 7221
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Tmem68
Name: transmembrane protein 68
Synonyms: 2010300G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 72098
Homologene: 12440
Shq1
Name: SHQ1 homolog (S. cerevisiae)
Synonyms: Grim-1, 2810403P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 72171
Homologene: 31683
Siglecg
Name: sialic acid binding Ig-like lectin G
Synonyms: mSiglec-G, A630096C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243958
Homologene: 13228
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74499
Homologene: 11542
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237553
Homologene: 75007
Fgf3
Name: fibroblast growth factor 3
Synonyms: Int-P, Fgf-3, Int-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14174
HGNC: HGNC:3681
Homologene: 3841
Sp8
Name: trans-acting transcription factor 8
Synonyms: D930049B17Rik, mBtd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 320145
VEGA: 12
Homologene: 18548
Cd82
Name: CD82 antigen
Synonyms: Kai1, Tspan27, C33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12521
HGNC: HGNC:6210
Homologene: 20512
Dock8
Name: dedicator of cytokinesis 8
Synonyms: A130095G14Rik, 1200017A24Rik, 5830472H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 76088
VEGA: 19
Homologene: 52414
Pik3r6
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: p84 Pikap, p87PIKAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 104709
Homologene: 46215
Sema3d
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms: 4631426B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 108151
Homologene: 23664
Pomgnt2
Name: protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2
Synonyms: Gtdc2, C85492
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 215494
VEGA: 9
Homologene: 32795
Pld2
Name: phospholipase D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18806
HGNC: HGNC:9068
Homologene: 55672
Sun1
Name: Sad1 and UNC84 domain containing 1
Synonyms: 4632417G13Rik, Unc84a, 5730434D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 77053
Homologene: 11544
Klhl25
Name: kelch-like 25
Synonyms: 2810402K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 207952
Homologene: 32546
Cmpk1
Name: cytidine monophosphate (UMP-CMP) kinase 1
Synonyms: 0610011D08Rik, Cmpk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66588
Homologene: 32308
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76261
Homologene: 49537
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 546913
Homologene: 84037
Vmn2r4
Name: vomeronasal 2, receptor 4
Synonyms: EG637053
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 637053
Homologene: 129754
Abcc1
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Synonyms: Abcc1a, Mrp1, Mdrap, MRP, Abcc1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Vmn2r109
Name: vomeronasal 2, receptor 109
Synonyms: EG627814
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627814
Homologene: 129678
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Olfml2a
Name: olfactomedin-like 2A
Synonyms: photomedin-1, 4932431K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241327
Homologene: 35248
Psg17
Name: pregnancy specific glycoprotein 17
Synonyms: Cea2, mmCGM5, Cea-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26437
Homologene: 110989
Rfk
Name: riboflavin kinase
Synonyms: 0610038L10Rik, ATP:riboflavin 5'-phosphotransferase, flavokinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 54391
Homologene: 115690
Ndufaf1
Name: NADH:ubiquinone oxidoreductase complex assembly factor 1
Synonyms: CIA30, CGI-65, 2410001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 69702
Homologene: 32289
Tmem181a
Name: transmembrane protein 181A
Synonyms: Gpr178, Tmem181, 5930418K15Rik, C76977
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 77106
Homologene: 44787
Olfr402
Name: olfactory receptor 402
Synonyms: MOR255-4, GA_x6K02T2P1NL-4307199-4308146
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258703
HGNC: HGNC:8282
Homologene: 1915
Mapk7
Name: mitogen-activated protein kinase 7
Synonyms: Erk5-T, big MAP kinase 1, ERK5, BMK1, b2b2346Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 23939
HGNC: HGNC:6880
Homologene: 2060
Tlcd1
Name: TLC domain containing 1
Synonyms: 0610007A15Rik, 0610030G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 68385
Homologene: 12263
Gm15561
Name: predicted gene 15561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 2 at 38,941,743 bp
  • A to T, chromosome 2 at 49,279,764 bp
  • T to C, chromosome 2 at 93,419,869 bp
  • A to G, chromosome 2 at 109,288,121 bp
  • A to G, chromosome 2 at 119,660,464 bp
  • A to G, chromosome 2 at 181,028,620 bp
  • A to G, chromosome 3 at 53,654,338 bp
  • C to T, chromosome 3 at 64,415,169 bp
  • A to T, chromosome 4 at 3,569,534 bp
  • T to C, chromosome 4 at 114,963,362 bp
  • A to G, chromosome 4 at 148,625,202 bp
  • T to C, chromosome 5 at 12,448,120 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • A to G, chromosome 5 at 108,136,077 bp
  • T to C, chromosome 5 at 139,234,475 bp
  • T to C, chromosome 6 at 100,670,928 bp
  • T to C, chromosome 6 at 123,637,858 bp
  • G to T, chromosome 7 at 18,816,792 bp
  • C to T, chromosome 7 at 43,417,926 bp
  • T to A, chromosome 7 at 75,865,414 bp
  • T to C, chromosome 7 at 144,840,707 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • T to C, chromosome 9 at 121,982,254 bp
  • A to T, chromosome 10 at 114,503,123 bp
  • T to C, chromosome 11 at 61,489,229 bp
  • A to G, chromosome 11 at 68,551,891 bp
  • A to T, chromosome 11 at 70,541,334 bp
  • T to C, chromosome 11 at 74,155,199 bp
  • C to T, chromosome 11 at 78,180,111 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • A to G, chromosome 12 at 44,576,775 bp
  • T to A, chromosome 12 at 69,268,226 bp
  • T to A, chromosome 12 at 118,849,203 bp
  • T to A, chromosome 13 at 73,627,475 bp
  • A to T, chromosome 15 at 9,116,947 bp
  • A to G, chromosome 15 at 100,983,903 bp
  • T to A, chromosome 16 at 14,445,300 bp
  • A to C, chromosome 16 at 33,961,586 bp
  • T to A, chromosome 16 at 38,193,936 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • T to C, chromosome 17 at 20,553,024 bp
  • T to A, chromosome 19 at 17,398,595 bp
  • T to C, chromosome 19 at 25,065,390 bp
  • T to C, chromosome 19 at 25,200,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4428 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041698-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.