Strain Name:
C57BL/6J-MtgxR4432Btlr/Mmmh
Stock Number:
041701-MU
Citation ID:
RRID:MMRRC_041701-MU
Other Names:
R4432 (G1), C57BL/6J-MtgxR4432Btlr
Major Collection:

Gene Information

Dnm2
Name: dynamin 2
Synonyms: b2b2159Clo, Dyn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13430
HGNC: HGNC:2974
Homologene: 90883
Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319582
Homologene: 35306
Ank2
Name: ankyrin 2, brain
Synonyms: Gm4392, Ank-2, Ankyrin-2, Ankyrin-B, ankyrin B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Mapkap1
Name: mitogen-activated protein kinase associated protein 1
Synonyms: Sin1, D230039K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227743
Homologene: 11473
Rasa2
Name: RAS p21 protein activator 2
Synonyms: GAP1m, 5430433H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Abcc5
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Synonyms: Abcc5b, Abcc5a, Mrp5, 2900011L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27416
HGNC: HGNC:56
Homologene: 21164
Rpl24
Name: ribosomal protein L24
Synonyms: Bst, 0610008L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 68193
Homologene: 763
Szt2
Name: SZT2 subunit of KICSTOR complex
Synonyms: seaizure threshold 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230676
Homologene: 49413
Pcolce2
Name: procollagen C-endopeptidase enhancer 2
Synonyms: 2400001O18Rik, Pcpe2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76477
HGNC: HGNC:8739
Homologene: 8357
Insc
Name: INSC spindle orientation adaptor protein
Synonyms: 3830422K02Rik, Inscuteable
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233752
Homologene: 78662
Chia1
Name: chitinase, acidic 1
Synonyms: Chia, YNL, AMCase, 2200003E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 81600
Homologene: 75168
Slc1a1
Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Synonyms: EAAT3, D130048G10Rik, MEAAC1, EAAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20510
Homologene: 20881
Phf11c
Name: PHD finger protein 11C
Synonyms: Gm6907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 628705
Homologene: 87807
Vmn1r218
Name: vomeronasal 1 receptor 218
Synonyms: V1ri5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171256
Homologene: 110880
Dnm3
Name: dynamin 3
Synonyms: 9630020E24Rik, B230343F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 103967
Homologene: 22906
Abca6
Name: ATP-binding cassette, sub-family A (ABC1), member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Pcid2
Name: PCI domain containing 2
Synonyms: A730042J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234069
Homologene: 10168
Adamtsl4
Name: ADAMTS-like 4
Synonyms: Tsrc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229595
Homologene: 23141
Prl8a8
Name: prolactin family 8, subfamily a, member 81
Synonyms: Prlpc3, PLP-Cgamma, 1600032B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74188
Homologene: 137338
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218989
Homologene: 49506
Cadps2
Name: Ca2+-dependent activator protein for secretion 2
Synonyms: cpd2, A230044C21Rik, Caps2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320405
Homologene: 23060
Cyp3a59
Name: cytochrome P450, family 3, subfamily a, polypeptide 59
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041449
Homologene: 135775
Pcdhb15
Name: protocadherin beta 15
Synonyms: Pcdhb7, PcdhbO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93886
HGNC: HGNC:8692
Homologene: 32429
Samhd1
Name: SAM domain and HD domain, 1
Synonyms: E330031J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56045
Homologene: 9160
Nmur1
Name: neuromedin U receptor 1
Synonyms: NmU-R, FM-3, NMU1R, Gpr66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14767
HGNC: HGNC:4518
Homologene: 68501
Lrrc45
Name: leucine rich repeat containing 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217366
Homologene: 17019
H6pd
Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Synonyms: G6pd1, Gpd-1, Gpd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100198
HGNC: HGNC:4795
Homologene: 48275
Cdk14
Name: cyclin-dependent kinase 14
Synonyms: Pftk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18647
HGNC: HGNC:8883
Homologene: 7888
Dpp4
Name: dipeptidylpeptidase 4
Synonyms: Cd26, Dpp-4, THAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13482
HGNC: HGNC:3009
Homologene: 3279
Slc27a3
Name: solute carrier family 27 (fatty acid transporter), member 3
Synonyms: Acsvl3, FATP3, fatty acid transport protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26568
Homologene: 11529
Anks6
Name: ankyrin repeat and sterile alpha motif domain containing 6
Synonyms: b2b1801.1Clo, LOC269533, Samd6, 2210417J20Rik, SamCystin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 75691
Homologene: 19545
Vmn2r32
Name: vomeronasal 2, receptor 32
Synonyms: V2r5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22311
Homologene: 113703
Cibar2
Name: CBY1 interacting BAR domain containing 2
Synonyms: Fam92b, 1700120B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 436062
Homologene: 78513
Acsm4
Name: acyl-CoA synthetase medium-chain family member 4
Synonyms: O-MACS, OMACS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233801
Homologene: 72281
Olfr1254
Name: olfactory receptor 1254
Synonyms: GA_x6K02T2Q125-51230155-51229211, MOR231-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258468
Homologene: 103784
Casp4
Name: caspase 4, apoptosis-related cysteine peptidase
Synonyms: ich-3, Caspase-11, Casp11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12363
HGNC: HGNC:1505
Homologene: 136493
Olfr1288
Name: olfactory receptor 1288
Synonyms: MOR245-9, GA_x6K02T2Q125-72530279-72531217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258395
Homologene: 88360
Hnrnpa0
Name: heterogeneous nuclear ribonucleoprotein A0
Synonyms: 3010025E17Rik, 1110055B05Rik, Hnrpa0
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 77134
VEGA: 13
HGNC: HGNC:5030
Homologene: 136809
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 86,387,565 bp
  • T to C, chromosome 1 at 161,991,997 bp
  • T to C, chromosome 2 at 34,619,863 bp
  • A to G, chromosome 2 at 62,345,112 bp
  • A to T, chromosome 2 at 89,788,734 bp
  • T to A, chromosome 2 at 111,479,412 bp
  • T to C, chromosome 2 at 157,104,893 bp
  • G to A, chromosome 3 at 90,387,340 bp
  • T to C, chromosome 3 at 95,681,759 bp
  • A to G, chromosome 3 at 106,115,325 bp
  • A to T, chromosome 3 at 126,947,806 bp
  • G to A, chromosome 4 at 47,044,905 bp
  • A to G, chromosome 4 at 118,384,231 bp
  • T to G, chromosome 4 at 149,995,758 bp
  • A to G, chromosome 5 at 5,036,427 bp
  • A to G, chromosome 5 at 146,104,786 bp
  • T to A, chromosome 6 at 23,626,738 bp
  • T to A, chromosome 7 at 7,479,919 bp
  • C to T, chromosome 7 at 114,769,055 bp
  • A to C, chromosome 7 at 119,711,387 bp
  • T to C, chromosome 8 at 13,085,421 bp
  • A to T, chromosome 8 at 36,498,478 bp
  • C to A, chromosome 8 at 120,174,855 bp
  • G to A, chromosome 9 at 5,323,653 bp
  • T to C, chromosome 9 at 21,491,304 bp
  • T to C, chromosome 9 at 95,681,557 bp
  • A to G, chromosome 9 at 96,542,407 bp
  • T to C, chromosome 11 at 110,241,588 bp
  • G to A, chromosome 11 at 120,715,221 bp
  • T to C, chromosome 13 at 23,137,242 bp
  • T to A, chromosome 13 at 27,510,480 bp
  • T to C, chromosome 13 at 58,127,937 bp
  • T to A, chromosome 14 at 14,757,323 bp
  • T to C, chromosome 14 at 48,446,467 bp
  • A to T, chromosome 14 at 59,390,935 bp
  • A to T, chromosome 16 at 20,368,187 bp
  • C to T, chromosome 16 at 55,966,616 bp
  • A to G, chromosome 18 at 37,475,512 bp
  • T to C, chromosome 19 at 28,902,709 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4432 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041701-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.