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Strain Name:
C57BL/6J-MtgxR4438Btlr/Mmmh
Stock Number:
041703-MU
Citation ID:
RRID:MMRRC_041703-MU
Other Names:
R4438 (G1), C57BL/6J-MtgxR4438Btlr
Major Collection:

Strain Information

Gpr83
Name: G protein-coupled receptor 83
Synonyms: RP105, RP82, RP39, glucocorticoid-induced receptor, GIR, Gpr72, Gir
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14608
HGNC: HGNC:4523
Homologene: 7732
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 2900022L22Rik, 7a5ex
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Csnk1e
Name: casein kinase 1, epsilon
Synonyms: CKI epsilon, tau, KC1epsilon, CKIepsilon, CK1epsilon
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 27373
Homologene: 121695
Zfp568
Name: zinc finger protein 568
Synonyms: LOC381866, chato
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243905
Homologene: 136307
Srfbp1
Name: serum response factor binding protein 1
Synonyms: 2810036K01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67222
VEGA: 18
Homologene: 12101
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Mtbp
Name: Mdm2, transformed 3T3 cell double minute p53 binding protein
Synonyms: MDM2BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105837
HGNC: HGNC:7417
Homologene: 11102
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 25,831,027 bp
  • T to C, chromosome 1 at 72,611,478 bp
  • C to A, chromosome 1 at 173,130,302 bp
  • A to C, chromosome 2 at 30,048,843 bp
  • T to A, chromosome 2 at 58,477,727 bp
  • A to T, chromosome 2 at 121,457,994 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to G, chromosome 2 at 177,265,096 bp
  • T to A, chromosome 3 at 90,777,875 bp
  • T to C, chromosome 3 at 94,402,897 bp
  • T to G, chromosome 3 at 129,284,091 bp
  • A to G, chromosome 4 at 32,704,635 bp
  • T to C, chromosome 4 at 64,007,829 bp
  • G to T, chromosome 4 at 96,217,409 bp
  • G to T, chromosome 4 at 106,458,959 bp
  • T to C, chromosome 4 at 143,991,622 bp
  • A to C, chromosome 5 at 24,446,070 bp
  • A to T, chromosome 5 at 43,825,340 bp
  • T to C, chromosome 5 at 65,395,444 bp
  • T to A, chromosome 5 at 139,163,392 bp
  • C to A, chromosome 6 at 7,675,320 bp
  • C to A, chromosome 6 at 24,505,868 bp
  • T to A, chromosome 6 at 40,884,062 bp
  • T to C, chromosome 6 at 43,116,287 bp
  • C to T, chromosome 6 at 48,487,353 bp
  • A to G, chromosome 6 at 123,839,797 bp
  • G to T, chromosome 7 at 30,022,296 bp
  • A to G, chromosome 7 at 35,692,859 bp
  • A to G, chromosome 7 at 127,785,731 bp
  • G to A, chromosome 7 at 130,172,930 bp
  • G to A, chromosome 7 at 130,623,541 bp
  • T to C, chromosome 9 at 14,864,838 bp
  • G to T, chromosome 9 at 30,428,599 bp
  • A to G, chromosome 9 at 83,839,813 bp
  • A to T, chromosome 9 at 88,731,700 bp
  • T to C, chromosome 9 at 110,885,398 bp
  • G to T, chromosome 10 at 38,965,987 bp
  • C to T, chromosome 12 at 88,431,150 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • T to C, chromosome 14 at 50,034,830 bp
  • A to T, chromosome 15 at 9,351,197 bp
  • G to A, chromosome 15 at 47,899,795 bp
  • C to A, chromosome 15 at 55,603,215 bp
  • A to T, chromosome 15 at 79,420,929 bp
  • T to C, chromosome 15 at 97,807,715 bp
  • G to T, chromosome 17 at 43,087,842 bp
  • T to C, chromosome 18 at 52,488,331 bp
  • C to A, chromosome 19 at 12,778,021 bp
  • T to C, chromosome 19 at 29,007,621 bp
  • A to T, chromosome 19 at 29,334,562 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4438 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041703-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.