Strain Name:
C57BL/6J-MtgxR4440Btlr/Mmmh
Stock Number:
041705-MU
Citation ID:
RRID:MMRRC_041705-MU
Other Names:
R4440 (G1), C57BL/6J-MtgxR4440Btlr
Major Collection:

Gene Information

Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: trkB, Tkrb, C030027L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C130067A03Rik, C530050H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320713
Homologene: 66204
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208628
Homologene: 32227
Hdac4
Name: histone deacetylase 4
Synonyms: 4932408F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208727
Homologene: 55946
Armc8
Name: armadillo repeat containing 8
Synonyms: 1200015K23Rik, Gid5, HSPC056
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74125
Homologene: 9121
Nav2
Name: neuron navigator 2
Synonyms: Rainb1, POMFIL2, HELAD1, 5330421F07Rik, Unc53H2, RAINB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Afdn
Name: afadin, adherens junction formation factor
Synonyms: 5033403D15Rik, Afadin, AF6, Mllt4, S-afadin, I-afadin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: 194kDa, RPA194, 3010014K16Rik, Rpo1-4, mRPA1, 2900087K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20019
Homologene: 7033
Fip1l1
Name: FIP1 like 1 (S. cerevisiae)
Synonyms: Rje, 1300019H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66899
Homologene: 136254
Tbl1xr1
Name: transducin (beta)-like 1X-linked receptor 1
Synonyms: TBLR1, A630076E03Rik, 8030499H02Rik, C230089I12Rik, Ira1, DC42, C21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 81004
Homologene: 69382
Adnp
Name: activity-dependent neuroprotective protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11538
Homologene: 7617
Tbr1
Name: T-box brain transcription factor 1
Synonyms: T-box brain gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21375
Homologene: 4807
C6
Name: complement component 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12274
HGNC: HGNC:1339
Homologene: 47
Nipbl
Name: NIPBL cohesin loading factor
Synonyms: 4933421G18Rik, 4921518A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71175
VEGA: 15
Homologene: 15850
Mideas
Name: mitotic deacetylase associated SANT domain protein
Synonyms: Elmsan1, C130039O16Rik, 9430029N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238317
Homologene: 19330
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Klhl8
Name: kelch-like 8
Synonyms: D5Ertd431e, 2310001P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 246293
Homologene: 10819
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75619
Homologene: 8957
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Cat2, Tea, Atrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11988
Homologene: 20659
Tespa1
Name: thymocyte expressed, positive selection associated 1
Synonyms: 5830405N20Rik, Itprid3, A430001F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67596
VEGA: 10
Homologene: 106645
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, Pwwp2, D7Ertd517e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101631
Homologene: 19056
Angpt4
Name: angiopoietin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11602
HGNC: HGNC:487
Homologene: 36302
Taok2
Name: TAO kinase 2
Synonyms: MAP3K17, TAO2, 1110033K02Rik, PSK1, TAO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381921
Homologene: 74531
Xab2
Name: XPA binding protein 2
Synonyms: 0610041O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67439
Homologene: 5738
Vmn1r231
Name: vomeronasal 1 receptor 231
Synonyms: V1re7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171230
Atg2a
Name: autophagy related 2A
Synonyms: 1810013C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329015
Homologene: 86985
Lpin3
Name: lipin 3
Synonyms: 9130206L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 64899
Homologene: 84844
Man2a2
Name: mannosidase 2, alpha 2
Synonyms: alpha mannosidase IIx, MX, 1700052O22Rik, 4931438M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 140481
HGNC: HGNC:6825
Homologene: 55954
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A5, UT-A3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
Pramel15
Name: PRAME like 15
Synonyms: Gm13125, Pramef20, EG627009
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 627009
Homologene: 133194
Nkiras1
Name: NFKB inhibitor interacting Ras-like protein 1
Synonyms: KBRAS1, 2400004O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69721
Homologene: 10670
Tex55
Name: testis expressed 55
Synonyms: 4930435E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74663
Homologene: 17614
Fpgs
Name: folylpolyglutamyl synthetase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14287
HGNC: HGNC:3824
Homologene: 6997
Olfr1212
Name: olfactory receptor 1212
Synonyms: MOR233-17, MOR233-20, GA_x6K02T2Q125-50437014-50437949
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258241
Homologene: 66154
Ctnna3
Name: catenin (cadherin associated protein), alpha 3
Synonyms: alphaT-catenin, Catna3, Vr22, 4930429L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216033
HGNC: HGNC:2511
Homologene: 56583
Ndufaf5
Name: NADH:ubiquinone oxidoreductase complex assembly factor 5
Synonyms: 2310003L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69487
Homologene: 11474
Tle2
Name: transducin-like enhancer of split 2
Synonyms: Grg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21886
Homologene: 20693
Cfc1
Name: cripto, FRL-1, cryptic family 1
Synonyms: b2b970Clo, cryptic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12627
Homologene: 50007
Alpl
Name: alkaline phosphatase, liver/bone/kidney
Synonyms: TNSALP, TNAP, ALP, Akp2, Akp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11647
HGNC: HGNC:438
Homologene: 37314
Olfr1248
Name: olfactory receptor 1248
Synonyms: MOR231-10, GA_x6K02T2Q125-51059648-51058725
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258787
Homologene: 133613
Dpysl5
Name: dihydropyrimidinase-like 5
Synonyms: CRMP-5, CRAM, Crmp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 65254
Homologene: 41347
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: Grf2, 6330417G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
Bicdl2
Name: BICD family like cargo adaptor 2
Synonyms: Ccdc64b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 212733
VEGA: 17
Homologene: 17815
Smok3c
Name: sperm motility kinase 3C
Synonyms: EG622486
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622486
Homologene: 128738
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,544,102 bp
  • G to A, chromosome 1 at 63,745,842 bp
  • C to A, chromosome 1 at 91,945,995 bp
  • C to T, chromosome 2 at 32,687,501 bp
  • A to T, chromosome 2 at 61,804,838 bp
  • A to G, chromosome 2 at 82,991,206 bp
  • G to A, chromosome 2 at 88,959,341 bp
  • G to T, chromosome 2 at 89,618,168 bp
  • T to A, chromosome 2 at 140,170,725 bp
  • G to T, chromosome 2 at 151,944,646 bp
  • A to G, chromosome 2 at 160,898,645 bp
  • G to T, chromosome 2 at 168,184,801 bp
  • A to G, chromosome 3 at 22,200,588 bp
  • T to C, chromosome 4 at 94,965,212 bp
  • A to T, chromosome 4 at 137,747,813 bp
  • A to T, chromosome 4 at 144,372,867 bp
  • T to C, chromosome 5 at 30,792,268 bp
  • T to C, chromosome 5 at 74,536,785 bp
  • A to G, chromosome 5 at 103,867,567 bp
  • T to C, chromosome 5 at 123,794,153 bp
  • T to A, chromosome 5 at 138,064,604 bp
  • A to G, chromosome 6 at 71,950,848 bp
  • C to T, chromosome 7 at 49,552,037 bp
  • A to G, chromosome 7 at 49,575,263 bp
  • C to A, chromosome 7 at 80,351,715 bp
  • G to A, chromosome 7 at 105,696,728 bp
  • C to T, chromosome 7 at 126,866,521 bp
  • T to C, chromosome 7 at 139,255,639 bp
  • T to C, chromosome 8 at 3,616,353 bp
  • T to C, chromosome 8 at 40,902,649 bp
  • T to A, chromosome 9 at 99,484,034 bp
  • A to G, chromosome 10 at 64,260,935 bp
  • A to G, chromosome 10 at 81,581,682 bp
  • C to T, chromosome 10 at 130,361,957 bp
  • C to T, chromosome 12 at 84,156,471 bp
  • G to C, chromosome 13 at 59,060,312 bp
  • T to C, chromosome 13 at 91,983,678 bp
  • C to T, chromosome 14 at 18,276,784 bp
  • A to T, chromosome 15 at 4,735,251 bp
  • G to C, chromosome 15 at 8,366,658 bp
  • T to C, chromosome 16 at 38,828,431 bp
  • T to C, chromosome 17 at 13,850,890 bp
  • T to C, chromosome 17 at 20,890,456 bp
  • C to T, chromosome 17 at 23,667,616 bp
  • A to G, chromosome 18 at 78,195,747 bp
  • T to C, chromosome 19 at 6,255,829 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041705-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.