Strain Name:
C57BL/6J-MtgxR4447Btlr/Mmmh
Stock Number:
041708-MU
Citation ID:
RRID:MMRRC_041708-MU
Other Names:
R4447 (G1), C57BL/6J-MtgxR4447Btlr
Major Collection:

Gene Information

Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: Cyp11b-2, Cyp11b, aldosterone synthase, steroid-11-beta-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13072
Homologene: 128035
Zfp407
Name: zinc finger protein 407
Synonyms: LOC381139, LOC240469, 6430585N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240476
Homologene: 86402
Rnpc3
Name: RNA-binding region (RNP1, RRM) containing 3
Synonyms: C030014B17Rik, 2810441O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67225
Homologene: 9746
Wwc2
Name: WW, C2 and coiled-coil domain containing 2
Synonyms: D8Ertd594e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52357
Homologene: 32618
Twsg1
Name: twisted gastrulation BMP signaling modulator 1
Synonyms: 9030422N06Rik, Tsg, 1810013J15Rik, D17Ertd403e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 65960
VEGA: 17
Homologene: 10774
Map2k3
Name: mitogen-activated protein kinase kinase 3
Synonyms: MAP kinase kinase 3, Prkmk3, MKK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26397
HGNC: HGNC:6843
Homologene: 56430
Acsf2
Name: acyl-CoA synthetase family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 264895
Homologene: 23519
Pomgnt1
Name: protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase
Synonyms: 4930467B06Rik, 0610016I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68273
Homologene: 9806
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
Tgm5
Name: transglutaminase 5
Synonyms: TGx, 2310007C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74176
Homologene: 20899
Mipol1
Name: mirror-image polydactyly 1
Synonyms: 1700081O04Rik, D12Ertd19e, 6030439O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73490
Homologene: 16340
Aldh6a1
Name: aldehyde dehydrogenase family 6, subfamily A1
Synonyms: 1110038I05Rik, Mmsdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104776
VEGA: 12
HGNC: HGNC:7179
Homologene: 4082
Olfr371
Name: olfactory receptor 371
Synonyms: MOR141-3, GA_x6K02T2NUPS-13298842-13299780
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 258858
Homologene: 65998
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5, SkM2, mH1, Nav1.5c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20271
Homologene: 22738
Atp1a4
Name: ATPase, Na+/K+ transporting, alpha 4 polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27222
Homologene: 113769
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 319695
Homologene: 85163
Iqcm
Name: IQ motif containing M
Synonyms: 1700007B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71831
Homologene: 53050
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: Prkwnk4, 2010002J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69847
Homologene: 13020
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330267
Homologene: 46582
Vmn1r228
Name: vomeronasal 1 receptor 228
Synonyms: V1re3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171226
Homologene: 74320
Irf5
Name: interferon regulatory factor 5
Synonyms: mirf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27056
HGNC: HGNC:6120
Homologene: 8088
3930402G23Rik
Name: RIKEN cDNA 3930402G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 665306
Rxfp1
Name: relaxin/insulin-like family peptide receptor 1
Synonyms: LOC381489, Lgr7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381489
Homologene: 11007
Alg11
Name: asparagine-linked glycosylation 11 (alpha-1,2-mannosyltransferase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 207958
Homologene: 68893
Niban1
Name: niban apoptosis regulator 1
Synonyms: Fam129a, Niban
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 63913
Homologene: 62170
Zscan29
Name: zinc finger SCAN domains 29
Synonyms: Zfp690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99334
Homologene: 17586
Zfp598
Name: zinc finger protein 598
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213753
VEGA: 17
Homologene: 5672
Mgst1
Name: microsomal glutathione S-transferase 1
Synonyms: 1500002K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56615
HGNC: HGNC:7061
Homologene: 10544
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20392
Homologene: 31205
Ano3
Name: anoctamin 3
Synonyms: Tmem16c, B230324K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
1700014D04Rik
Name: RIKEN cDNA 1700014D04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 102638268
Homologene: 140986
Ubqln3
Name: ubiquilin 3
Synonyms: 4933400K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244178
Homologene: 9700
RP24-153E5.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 72,687,789 bp
  • A to T, chromosome 1 at 75,470,370 bp
  • A to T, chromosome 1 at 151,636,402 bp
  • A to C, chromosome 1 at 172,234,431 bp
  • A to T, chromosome 2 at 110,761,578 bp
  • G to T, chromosome 2 at 121,077,537 bp
  • T to A, chromosome 2 at 121,169,886 bp
  • C to T, chromosome 3 at 79,652,127 bp
  • T to C, chromosome 3 at 113,611,137 bp
  • G to T, chromosome 4 at 116,152,923 bp
  • A to G, chromosome 6 at 4,717,930 bp
  • G to A, chromosome 6 at 12,324,635 bp
  • T to A, chromosome 6 at 29,535,942 bp
  • G to T, chromosome 6 at 138,141,664 bp
  • C to T, chromosome 7 at 104,142,814 bp
  • T to A, chromosome 8 at 10,926,129 bp
  • C to A, chromosome 8 at 22,068,079 bp
  • A to G, chromosome 8 at 47,868,667 bp
  • T to C, chromosome 8 at 75,629,766 bp
  • T to A, chromosome 8 at 85,231,366 bp
  • A to G, chromosome 8 at 124,295,377 bp
  • T to C, chromosome 9 at 119,550,627 bp
  • A to T, chromosome 11 at 60,947,171 bp
  • G to T, chromosome 11 at 94,569,359 bp
  • T to A, chromosome 11 at 101,268,451 bp
  • G to T, chromosome 12 at 57,352,748 bp
  • A to G, chromosome 12 at 84,439,709 bp
  • T to C, chromosome 13 at 59,742,198 bp
  • T to C, chromosome 15 at 74,855,563 bp
  • T to C, chromosome 17 at 20,777,107 bp
  • T to A, chromosome 17 at 24,676,555 bp
  • C to T, chromosome 17 at 65,929,787 bp
  • A to T, chromosome 18 at 84,562,694 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4447 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041708-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.