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Strain Name:
C57BL/6J-MtgxR4448Btlr/Mmmh
Stock Number:
041709-MU
Citation ID:
RRID:MMRRC_041709-MU
Other Names:
R4448 (G1), C57BL/6J-MtgxR4448Btlr
Major Collection:

Strain Information

Ctcf
Name: CCCTC-binding factor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13018
Homologene: 4786
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Ezr
Name: ezrin
Synonyms: ezrin, p81, cytovillin, Vil2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22350
Homologene: 55740
Taf4
Name: TATA-box binding protein associated factor 4
Synonyms: Taf2c1, TAFII130, TAFII135, Taf4a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228980
Homologene: 55723
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, D15F32S1h, D7H15F37S1, rjs, jdf2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Urb1
Name: URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Synonyms: 5730405K23Rik, 4921511H13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207932
Homologene: 45941
Rbm26
Name: RNA binding motif protein 26
Synonyms: Pro1777, C230097K14Rik, 1700009P03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74213
Homologene: 41468
Sec23b
Name: SEC23 homolog B, COPII coat complex component
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27054
Homologene: 74571
Wwc2
Name: WW, C2 and coiled-coil domain containing 2
Synonyms: D8Ertd594e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52357
Homologene: 32618
Atp6v1b2
Name: ATPase, H+ transporting, lysosomal V1 subunit B2
Synonyms: HO57, Atp6b2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11966
HGNC: HGNC:854
Homologene: 1279
Gpr4
Name: G protein-coupled receptor 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319197
HGNC: HGNC:4497
Homologene: 3867
Efs
Name: embryonal Fyn-associated substrate
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13644
VEGA: 14
Homologene: 4284
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: acetyl-CoA carboxylase, Acc1, LOC327983, Acac, A530025K05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Epg5
Name: ectopic P-granules 5 autophagy tethering factor
Synonyms: 5430411K18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Hhip
Name: Hedgehog-interacting protein
Synonyms: Hip, Hip1, Hhip1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15245
Homologene: 32469
Sqstm1
Name: sequestosome 1
Synonyms: A170, OSF-6, STAP, Osi, p62
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18412
Homologene: 31202
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: C130069N12Rik, PTR4, PHT1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100561
Homologene: 26432
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Rab38
Name: RAB38, member RAS oncogene family
Synonyms: 2310011F14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72433
HGNC: HGNC:9776
Homologene: 21353
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
F5
Name: coagulation factor V
Synonyms: Cf-5, Cf5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Asb8
Name: ankyrin repeat and SOCS box-containing 8
Synonyms: C430011H06Rik, 4930539L19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78541
Homologene: 32572
Kcnh4
Name: potassium voltage-gated channel, subfamily H (eag-related), member 4
Synonyms: BEC2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380728
HGNC: HGNC:6253
Homologene: 8180
Nckap5
Name: NCK-associated protein 5
Synonyms: LOC380609, D130011D22Rik, E030049G20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210356
Homologene: 35542
Iqcm
Name: IQ motif containing M
Synonyms: 1700007B14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71831
Homologene: 53050
Kansl1l
Name: KAT8 regulatory NSL complex subunit 1-like
Synonyms: C430010P07Rik, 1110028C15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68691
Homologene: 27376
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215772
Homologene: 100289
3930402G23Rik
Name: RIKEN cDNA 3930402G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 665306
Ankrd42
Name: ankyrin repeat domain 42
Synonyms: 4933417L02Rik, Ikbn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73845
Homologene: 23652
Vmn1r222
Name: vomeronasal 1 receptor 222
Synonyms: V1rh16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171274
Homologene: 110880
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Alg11
Name: ALG11 alpha-1,2-mannosyltransferase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 207958
Homologene: 68893
A1cf
Name: APOBEC1 complementation factor
Synonyms: ACF, apobec-1 complementation factor, 1810073H04Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69865
VEGA: 19
Homologene: 16363
Fcna
Name: ficolin A
Synonyms: ficolin A, Fcn1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14133
Homologene: 117949
Pag1
Name: phosphoprotein associated with glycosphingolipid microdomains 1
Synonyms: F730007C19Rik, Cbp
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 94212
Homologene: 10198
BC048562
Name: cDNA sequence BC048562
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 434439
Homologene: 79041
Hoxc12
Name: homeobox C12
Synonyms: Hox-3.8
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 15421
HGNC: HGNC:5124
Homologene: 7769
Fut7
Name: fucosyltransferase 7
Synonyms: Fuc-TVII, FTVII, FucT-VII
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14347
HGNC: HGNC:4018
Homologene: 3296
Fahd2a
Name: fumarylacetoacetate hydrolase domain containing 2A
Synonyms: 1500003K14Rik, B430104H02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68126
Homologene: 131290
Pttg1
Name: pituitary tumor-transforming gene 1
Synonyms: PTTG, securin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 30939
Homologene: 3110
Epn3
Name: epsin 3
Synonyms: 2310022G12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71889
Homologene: 56791
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 66,738,159 bp
  • G to A, chromosome 1 at 126,025,726 bp
  • A to T, chromosome 1 at 164,198,899 bp
  • A to G, chromosome 2 at 25,424,939 bp
  • A to G, chromosome 2 at 25,625,476 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to A, chromosome 2 at 127,438,174 bp
  • A to G, chromosome 2 at 144,559,251 bp
  • A to G, chromosome 2 at 179,935,971 bp
  • T to C, chromosome 3 at 9,699,466 bp
  • A to C, chromosome 4 at 144,332,685 bp
  • T to C, chromosome 5 at 23,464,790 bp
  • G to A, chromosome 5 at 107,601,795 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • A to G, chromosome 6 at 4,717,930 bp
  • G to A, chromosome 7 at 19,223,001 bp
  • T to C, chromosome 7 at 56,227,892 bp
  • T to A, chromosome 7 at 75,742,760 bp
  • T to G, chromosome 7 at 88,490,625 bp
  • G to A, chromosome 7 at 92,610,634 bp
  • T to A, chromosome 8 at 10,926,129 bp
  • C to A, chromosome 8 at 22,068,079 bp
  • A to G, chromosome 8 at 47,868,667 bp
  • T to A, chromosome 8 at 69,102,022 bp
  • T to C, chromosome 8 at 75,629,766 bp
  • C to T, chromosome 8 at 80,043,945 bp
  • A to T, chromosome 8 at 105,680,293 bp
  • A to G, chromosome 8 at 124,295,377 bp
  • A to T, chromosome 8 at 125,492,355 bp
  • T to A, chromosome 9 at 108,438,524 bp
  • T to C, chromosome 10 at 10,390,825 bp
  • A to T, chromosome 11 at 43,424,690 bp
  • A to G, chromosome 11 at 50,203,039 bp
  • A to T, chromosome 11 at 84,262,492 bp
  • A to T, chromosome 11 at 94,491,478 bp
  • A to G, chromosome 11 at 100,755,907 bp
  • G to A, chromosome 12 at 82,341,750 bp
  • A to C, chromosome 13 at 23,232,293 bp
  • G to A, chromosome 13 at 23,232,660 bp
  • A to G, chromosome 14 at 54,920,192 bp
  • T to C, chromosome 14 at 103,188,502 bp
  • A to T, chromosome 14 at 105,151,550 bp
  • A to G, chromosome 15 at 98,141,330 bp
  • A to G, chromosome 15 at 102,938,476 bp
  • C to T, chromosome 16 at 90,769,394 bp
  • T to C, chromosome 17 at 6,753,074 bp
  • C to T, chromosome 17 at 43,629,735 bp
  • T to A, chromosome 18 at 77,962,461 bp
  • A to T, chromosome 19 at 31,945,862 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4448 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041709-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.