Strain Name:
C57BL/6J-MtgxR4450Btlr/Mmmh
Stock Number:
041711-MU
Citation ID:
RRID:MMRRC_041711-MU
Other Names:
R4450 (G1), C57BL/6J-MtgxR4450Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Syt6
Name: synaptotagmin VI
Synonyms: 3110037A08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54524
Homologene: 10301
Plaat1
Name: phospholipase A and acyltransferase 1
Synonyms: A-C1, 2810012B06Rik, Hrasls
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27281
Homologene: 8452
Arhgef18
Name: Rho/Rac guanine nucleotide exchange factor 18
Synonyms: D030053O22Rik, A430078G23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102098
Homologene: 32254
Cdca4
Name: cell division cycle associated 4
Synonyms: 2410018C03Rik, Hepp, SEI-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71963
VEGA: 12
Homologene: 9936
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, D15F32S1h, D7H15F37S1, rjs, jdf2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Rnpc3
Name: RNA-binding region (RNP1, RRM) containing 3
Synonyms: C030014B17Rik, 2810441O16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67225
Homologene: 9746
Dcp1b
Name: decapping mRNA 1B
Synonyms: B930050E02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319618
Homologene: 51881
Wwc2
Name: WW, C2 and coiled-coil domain containing 2
Synonyms: D8Ertd594e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52357
Homologene: 32618
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: C130069N12Rik, PTR4, PHT1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100561
Homologene: 26432
Map4k3
Name: mitogen-activated protein kinase kinase kinase kinase 3
Synonyms: 9530052P13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 225028
VEGA: 17
HGNC: HGNC:6865
Homologene: 2683
Nbeal1
Name: neurobeachin like 1
Synonyms: ALS2CR17, A530083I02Rik, A530050O19Rik, 2310076G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269198
Homologene: 16453
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
Trim58
Name: tripartite motif-containing 58
Synonyms: LOC216781, LOC386443
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216781
Homologene: 9135
Eln
Name: elastin
Synonyms: tropoelastin, E030024M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13717
HGNC: HGNC:3327
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Or8g34
Name: olfactory receptor family 8 subfamily G member 34
Synonyms: GA_x6K02T2PVTD-33158015-33158950, MOR171-53, MOR171-42, Olfr954
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258328
VEGA: 9
HGNC: HGNC:8484
Homologene: 71961
Klhl42
Name: kelch-like 42
Synonyms: C230080I20Rik, Klhdc5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232539
Homologene: 45842
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Lzts2
Name: leucine zipper, putative tumor suppressor 2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226154
VEGA: 19
Homologene: 15690
Iqcm
Name: IQ motif containing M
Synonyms: 1700007B14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71831
Homologene: 53050
Cep162
Name: centrosomal protein 162
Synonyms: 4922501C03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382090
Homologene: 8930
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Col6a5, Gm7455
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 665033
Homologene: 122792
Nsun4
Name: NOL1/NOP2/Sun domain family, member 4
Synonyms: 2310010O12Rik, 2810405F18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72181
Homologene: 12455
3930402G23Rik
Name: RIKEN cDNA 3930402G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 665306
Bpifb6
Name: BPI fold containing family B, member 6
Synonyms: LOC228796, Bpil3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228796
Homologene: 18375
Adamdec1
Name: ADAM-like, decysin 1
Synonyms: 2210414L24Rik, Dcsn
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 58860
VEGA: 14
Homologene: 8711
Osbpl5
Name: oxysterol binding protein-like 5
Synonyms: Obph1, ORP5, 1110006M06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 79196
Homologene: 98024
Alg11
Name: ALG11 alpha-1,2-mannosyltransferase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 207958
Homologene: 68893
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Plpp5
Name: phospholipid phosphatase 5
Synonyms: 1810019D05Rik, 2310022A04Rik, Ppapdc1b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71910
Homologene: 100569
Gm9894
Name: predicted gene 9894
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100417934
VEGA: 13
Acsl6
Name: acyl-CoA synthetase long-chain family member 6
Synonyms: Lacsl, A330035H04Rik, Facl6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216739
Homologene: 100939
Angel1
Name: angel homolog 1
Synonyms: 1110030H02Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68737
Homologene: 32251
Or8b52
Name: olfactory receptor family 8 subfamily B member 52
Synonyms: GA_x6K02T2PVTD-32368166-32367237, MOR168-2P, Olfr917
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258183
VEGA: 9
Rangrf
Name: RAN guanine nucleotide release factor
Synonyms: Mog1, Rangnrf, 2400006H24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57785
Homologene: 41138
Mlc1
Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)
Synonyms: Kiaa0027-hp, WKL1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170790
Homologene: 15775
Cldn12
Name: claudin 12
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 64945
HGNC: HGNC:2034
Homologene: 40809
Speer3
Name: spermatogenesis associated glutamate (E)-rich protein 3
Synonyms: SPEER-3, 4933405P08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71026
Homologene: 69402
Slc11a1
Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Synonyms: host resistance locus Bcg/Ity/Lsh, Nramp, Lsh, Bcg, Ity, Nramp1, ity, Ity1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18173
Homologene: 73884
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,322,035 bp
  • T to A, chromosome 1 at 60,267,774 bp
  • C to A, chromosome 1 at 74,385,535 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • A to G, chromosome 2 at 153,906,768 bp
  • A to G, chromosome 3 at 103,585,645 bp
  • T to C, chromosome 3 at 113,611,137 bp
  • A to T, chromosome 4 at 116,051,256 bp
  • T to C, chromosome 5 at 5,508,398 bp
  • C to G, chromosome 5 at 13,796,380 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • C to T, chromosome 5 at 134,502,953 bp
  • C to T, chromosome 5 at 134,725,781 bp
  • T to G, chromosome 5 at 150,536,053 bp
  • A to G, chromosome 6 at 119,206,476 bp
  • G to A, chromosome 6 at 147,091,671 bp
  • T to C, chromosome 7 at 56,227,892 bp
  • T to A, chromosome 7 at 75,742,760 bp
  • T to A, chromosome 7 at 143,694,906 bp
  • A to G, chromosome 8 at 3,437,097 bp
  • T to A, chromosome 8 at 10,926,129 bp
  • C to A, chromosome 8 at 22,068,079 bp
  • T to C, chromosome 8 at 25,722,578 bp
  • A to G, chromosome 8 at 47,868,667 bp
  • T to C, chromosome 8 at 75,629,766 bp
  • A to G, chromosome 8 at 124,295,377 bp
  • A to T, chromosome 9 at 38,665,754 bp
  • G to A, chromosome 9 at 39,462,032 bp
  • C to T, chromosome 9 at 67,344,065 bp
  • T to A, chromosome 9 at 75,167,176 bp
  • T to C, chromosome 9 at 87,225,808 bp
  • C to T, chromosome 9 at 105,904,521 bp
  • C to T, chromosome 10 at 52,077,942 bp
  • A to G, chromosome 11 at 54,328,403 bp
  • T to C, chromosome 11 at 58,651,365 bp
  • A to T, chromosome 11 at 68,975,184 bp
  • A to T, chromosome 12 at 86,721,924 bp
  • T to C, chromosome 12 at 112,821,658 bp
  • T to A, chromosome 13 at 67,765,080 bp
  • C to T, chromosome 14 at 68,573,119 bp
  • A to G, chromosome 15 at 88,963,490 bp
  • C to T, chromosome 16 at 29,228,224 bp
  • C to T, chromosome 17 at 80,603,982 bp
  • A to T, chromosome 19 at 45,023,593 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4450 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041711-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.