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Strain Name:
C57BL/6J-MtgxR4458Btlr/Mmmh
Stock Number:
041718-MU
Citation ID:
RRID:MMRRC_041718-MU
Other Names:
R4458 (G1), C57BL/6J-MtgxR4458Btlr
Major Collection:

Strain Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Th
Name: tyrosine hydroxylase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21823
Homologene: 307
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Prdm16
Name: PR domain containing 16
Synonyms: Mel1, 5730557K01Rik, line 27, csp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70673
Homologene: 11139
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 2900022L22Rik, 7a5ex
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,183,196 bp
  • A to T, chromosome 1 at 54,762,391 bp
  • A to G, chromosome 1 at 75,274,497 bp
  • T to C, chromosome 2 at 26,892,709 bp
  • A to G, chromosome 2 at 34,945,433 bp
  • T to C, chromosome 2 at 76,946,913 bp
  • G to T, chromosome 2 at 82,990,776 bp
  • T to A, chromosome 2 at 126,146,268 bp
  • T to A, chromosome 2 at 161,029,876 bp
  • G to A, chromosome 2 at 169,885,088 bp
  • G to T, chromosome 2 at 178,069,779 bp
  • T to C, chromosome 3 at 89,408,934 bp
  • G to A, chromosome 3 at 107,232,681 bp
  • A to C, chromosome 3 at 108,394,997 bp
  • A to G, chromosome 4 at 41,115,888 bp
  • C to T, chromosome 4 at 134,074,894 bp
  • A to G, chromosome 4 at 154,322,308 bp
  • T to A, chromosome 5 at 31,061,226 bp
  • T to C, chromosome 5 at 65,394,040 bp
  • T to C, chromosome 5 at 124,121,376 bp
  • T to A, chromosome 5 at 149,214,152 bp
  • G to A, chromosome 6 at 117,808,909 bp
  • G to T, chromosome 7 at 19,393,846 bp
  • A to G, chromosome 7 at 23,866,220 bp
  • G to A, chromosome 7 at 30,720,225 bp
  • A to G, chromosome 7 at 75,739,465 bp
  • A to G, chromosome 7 at 104,149,189 bp
  • G to T, chromosome 7 at 122,135,080 bp
  • A to T, chromosome 7 at 142,896,953 bp
  • T to A, chromosome 8 at 33,294,998 bp
  • T to C, chromosome 8 at 34,154,716 bp
  • C to A, chromosome 8 at 66,456,171 bp
  • T to A, chromosome 8 at 70,452,692 bp
  • G to T, chromosome 8 at 88,719,301 bp
  • G to A, chromosome 8 at 106,991,607 bp
  • T to A, chromosome 8 at 111,075,470 bp
  • A to G, chromosome 8 at 123,247,748 bp
  • C to G, chromosome 9 at 21,290,889 bp
  • C to T, chromosome 9 at 40,303,581 bp
  • T to C, chromosome 9 at 85,326,474 bp
  • T to C, chromosome 10 at 23,865,669 bp
  • GAA to GA, chromosome 10 at 67,539,903 bp
  • T to A, chromosome 11 at 20,446,476 bp
  • A to G, chromosome 11 at 93,870,533 bp
  • A to T, chromosome 11 at 97,169,170 bp
  • T to C, chromosome 11 at 102,000,574 bp
  • T to A, chromosome 12 at 8,015,445 bp
  • T to C, chromosome 12 at 44,559,730 bp
  • T to A, chromosome 12 at 52,517,957 bp
  • T to A, chromosome 13 at 100,154,911 bp
  • C to A, chromosome 13 at 103,833,557 bp
  • C to A, chromosome 14 at 62,674,473 bp
  • C to T, chromosome 15 at 103,350,203 bp
  • T to C, chromosome 16 at 20,213,321 bp
  • A to T, chromosome 17 at 5,242,916 bp
  • A to G, chromosome 17 at 20,814,632 bp
  • G to A, chromosome 17 at 23,581,176 bp
  • A to T, chromosome 17 at 25,785,020 bp
  • G to T, chromosome 17 at 37,067,775 bp
  • A to T, chromosome 17 at 37,211,313 bp
  • A to T, chromosome 17 at 75,276,507 bp
  • T to C, chromosome 18 at 22,751,995 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4458 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041718-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.