Strain Name:
C57BL/6J-MtgxR4458Btlr/Mmmh
Stock Number:
041718-MU
Citation ID:
RRID:MMRRC_041718-MU
Other Names:
R4458 (G1), C57BL/6J-MtgxR4458Btlr
Major Collection:

Strain Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Th
Name: tyrosine hydroxylase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21823
Homologene: 307
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: C030017F07Rik, Bravo, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Prdm16
Name: PR domain containing 16
Synonyms: 5730557K01Rik, csp1, line 27, Mel1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70673
Homologene: 11139
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 7a5ex, 2900022L22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Resp18
Name: regulated endocrine-specific protein 18
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19711
Homologene: 7516
Sntb2
Name: syntrophin, basic 2
Synonyms: Snt2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20650
Homologene: 4911
Zfp13
Name: zinc finger protein 13
Synonyms: Zfp-13, Krox-8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22654
VEGA: 17
Homologene: 2580
Itga5
Name: integrin alpha 5 (fibronectin receptor alpha)
Synonyms: Cd49e, Fnra
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16402
VEGA: 15
HGNC: HGNC:6141
Homologene: 20508
Kpnb1
Name: karyopherin subunit beta 1
Synonyms: Impnb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16211
HGNC: HGNC:6400
Homologene: 1707
Yeats2
Name: YEATS domain containing 2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208146
VEGA: 16
Homologene: 9967
Erbin
Name: Erbb2 interacting protein
Synonyms: 1700028E05Rik, Erbb2ip, Erbin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 59079
Homologene: 41282
Cad
Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms: 2410008J01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69719
HGNC: HGNC:1424
Homologene: 1412
Akap13
Name: A kinase anchor protein 13
Synonyms: 5730522G15Rik, 1700026G02Rik, PROTO-LB, Ht31, PROTO-LBC, 5830460E08Rik, AKAP-Lbc
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Utp18
Name: UTP18 small subunit processome component
Synonyms: 6230425C22Rik, Wdr50
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217109
Homologene: 41087
Arid1b
Name: AT-rich interaction domain 1B
Synonyms: 9330189K18Rik, B230217J03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 239985
Homologene: 32344
Nol6
Name: nucleolar protein family 6 (RNA-associated)
Synonyms: Nrap
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230082
Homologene: 41505
Gabbr1
Name: gamma-aminobutyric acid type B receptor subunit 1
Synonyms: GABAB1, GABAbR1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 54393
HGNC: HGNC:4070
Homologene: 1132
Apob
Name: apolipoprotein B
Synonyms: apob-48, apob-100
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Surf6
Name: surfeit gene 6
Synonyms: D2Wsu129e, Surf-6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20935
Homologene: 133981
Zfp931
Name: zinc finger protein 931
Synonyms: 2810021G02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 353208
Homologene: 136272
Clec18a
Name: C-type lectin domain family 18, member A
Synonyms: Mrcl
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 353287
Homologene: 18755
Mpp3
Name: membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Synonyms: Dlgh3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13384
HGNC: HGNC:7221
Homologene: 31065
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 6330406J24Rik, 5430439G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71389
Homologene: 32772
Klhl26
Name: kelch-like 26
Synonyms: C630013N10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234378
Homologene: 41247
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: 9430031G15Rik, LTBP-1, b2b1000Clo, Ltbp1L
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: EGFL2, mfmi1, Adgrc2, flamingo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Naip2
Name: NLR family, apoptosis inhibitory protein 2
Synonyms: Naip-rs6, Naip2, Birc1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17948
HGNC: HGNC:7634
Homologene: 136092
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Uspl1
Name: ubiquitin specific peptidase like 1
Synonyms: E430026A01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231915
Homologene: 4235
Gramd1b
Name: GRAM domain containing 1B
Synonyms: A930008A22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235283
Homologene: 18223
Arhgap5
Name: Rho GTPase activating protein 5
Synonyms: p190-B, p190B
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11855
VEGA: 12
HGNC: HGNC:675
Homologene: 907
Egr2
Name: early growth response 2
Synonyms: Egr-2, Krox20, Krox-20, Zfp-25, NGF1-B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13654
HGNC: HGNC:3239
Homologene: 20123
Pitpnm2
Name: phosphatidylinositol transfer protein, membrane-associated 2
Synonyms: Rdgb2, RDGBA2, NIR3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19679
Homologene: 7915
Flad1
Name: flavin adenine dinucleotide synthetase 1
Synonyms: A930017E24Rik, Pp591
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 319945
Homologene: 115598
Ubqlnl
Name: ubiquilin-like
Synonyms: LOC244179, 4922504M18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244179
Homologene: 17034
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75823
Homologene: 27384
Tshz2
Name: teashirt zinc finger family member 2
Synonyms: 2900073F20Rik, Tsh2, Mtsh2, Sdccag33l, Zfp218, teashirt2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228911
Homologene: 35258
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230806
Homologene: 19232
Nol4
Name: nucleolar protein 4
Synonyms: 4930568N03Rik, LOC383304, 1700013J13Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 319211
HGNC: HGNC:7870
Homologene: 36142
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: 2900009M21Rik, C130039D01Rik, CYLD1, 2010013M14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Traf1
Name: TNF receptor-associated factor 1
Synonyms: 4732496E14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22029
Homologene: 4138
Ercc2
Name: excision repair cross-complementing rodent repair deficiency, complementation group 2
Synonyms: Mhdarco15, XPD, Ercc-2, RCO015
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13871
HGNC: HGNC:3434
Homologene: 344
Vnn3
Name: vanin 3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 26464
Homologene: 48295
Marchf1
Name: membrane associated ring-CH-type finger 1
Synonyms: 2900024D24Rik, March1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72925
Homologene: 113749
Vmn1r177
Name: vomeronasal 1 receptor 177
Synonyms: V1rd12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384572
Homologene: 122945
Ankrd44
Name: ankyrin repeat domain 44
Synonyms: E130014H08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329154
Homologene: 27547
Tent5a
Name: terminal nucleotidyltransferase 5A
Synonyms: BAP014, D930050G01Rik, Fam46a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 212943
Homologene: 23032
Vps9d1
Name: VPS9 domain containing 1
Synonyms: 1300018I17Rik, 2410004N05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72325
Homologene: 21021
Or10c1
Name: olfactory receptor family 10 subfamily C member 1
Synonyms: Olfr95, GA_x6K02T2PSCP-1651760-1650822, MOR263-6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258506
HGNC: HGNC:8165
Homologene: 71990
Vmn1r229
Name: vomeronasal 1 receptor 229
Synonyms: V1re1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171224
Homologene: 74319
Dctn5
Name: dynactin 5
Synonyms: b2b315Clo, p25 dynactin subunit, 4930427E12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 59288
Homologene: 10998
Serpine3
Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 3
Synonyms: E130113E03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319433
VEGA: 14
Homologene: 53117
Saraf
Name: store-operated calcium entry-associated regulatory factor
Synonyms: Tmem66, 1810045K07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67887
Homologene: 9398
Haghl
Name: hydroxyacylglutathione hydrolase-like
Synonyms: 2810014I23Rik, C330022E15Rik, 1500017E18Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68977
Homologene: 57062
Gm12034
Name: predicted gene 12034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100502908
Prok1
Name: prokineticin 1
Synonyms: EG-VEGF
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 246691
Homologene: 13023
Gemin6-ps
Name: gem nuclear organelle associated protein 6, pseudogene
Synonyms: Gm6253
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 621705
Homologene: 11711
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,183,196 bp
  • A to T, chromosome 1 at 54,762,391 bp
  • A to G, chromosome 1 at 75,274,497 bp
  • T to C, chromosome 2 at 26,892,709 bp
  • A to G, chromosome 2 at 34,945,433 bp
  • T to C, chromosome 2 at 76,946,913 bp
  • G to T, chromosome 2 at 82,990,776 bp
  • T to A, chromosome 2 at 126,146,268 bp
  • T to A, chromosome 2 at 161,029,876 bp
  • G to A, chromosome 2 at 169,885,088 bp
  • G to T, chromosome 2 at 178,069,779 bp
  • T to C, chromosome 3 at 89,408,934 bp
  • G to A, chromosome 3 at 107,232,681 bp
  • A to C, chromosome 3 at 108,394,997 bp
  • A to G, chromosome 4 at 41,115,888 bp
  • C to T, chromosome 4 at 134,074,894 bp
  • A to G, chromosome 4 at 154,322,308 bp
  • T to A, chromosome 5 at 31,061,226 bp
  • T to C, chromosome 5 at 65,394,040 bp
  • T to C, chromosome 5 at 124,121,376 bp
  • T to A, chromosome 5 at 149,214,152 bp
  • G to A, chromosome 6 at 117,808,909 bp
  • G to T, chromosome 7 at 19,393,846 bp
  • A to G, chromosome 7 at 23,866,220 bp
  • G to A, chromosome 7 at 30,720,225 bp
  • A to G, chromosome 7 at 75,739,465 bp
  • A to G, chromosome 7 at 104,149,189 bp
  • G to T, chromosome 7 at 122,135,080 bp
  • A to T, chromosome 7 at 142,896,953 bp
  • T to A, chromosome 8 at 33,294,998 bp
  • T to C, chromosome 8 at 34,154,716 bp
  • C to A, chromosome 8 at 66,456,171 bp
  • T to A, chromosome 8 at 70,452,692 bp
  • G to T, chromosome 8 at 88,719,301 bp
  • G to A, chromosome 8 at 106,991,607 bp
  • T to A, chromosome 8 at 111,075,470 bp
  • A to G, chromosome 8 at 123,247,748 bp
  • C to G, chromosome 9 at 21,290,889 bp
  • C to T, chromosome 9 at 40,303,581 bp
  • T to C, chromosome 9 at 85,326,474 bp
  • T to C, chromosome 10 at 23,865,669 bp
  • GAA to GA, chromosome 10 at 67,539,903 bp
  • T to A, chromosome 11 at 20,446,476 bp
  • A to G, chromosome 11 at 93,870,533 bp
  • A to T, chromosome 11 at 97,169,170 bp
  • T to C, chromosome 11 at 102,000,574 bp
  • T to A, chromosome 12 at 8,015,445 bp
  • T to C, chromosome 12 at 44,559,730 bp
  • T to A, chromosome 12 at 52,517,957 bp
  • T to A, chromosome 13 at 100,154,911 bp
  • C to A, chromosome 13 at 103,833,557 bp
  • C to A, chromosome 14 at 62,674,473 bp
  • C to T, chromosome 15 at 103,350,203 bp
  • T to C, chromosome 16 at 20,213,321 bp
  • A to T, chromosome 17 at 5,242,916 bp
  • A to G, chromosome 17 at 20,814,632 bp
  • G to A, chromosome 17 at 23,581,176 bp
  • A to T, chromosome 17 at 25,785,020 bp
  • G to T, chromosome 17 at 37,067,775 bp
  • A to T, chromosome 17 at 37,211,313 bp
  • A to T, chromosome 17 at 75,276,507 bp
  • T to C, chromosome 18 at 22,751,995 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4458 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041718-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.