Strain Name:
C57BL/6J-MtgxR4461Btlr/Mmmh
Stock Number:
041720-MU
Citation ID:
RRID:MMRRC_041720-MU
Other Names:
R4461 (G1), C57BL/6J-MtgxR4461Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11287
Homologene: 104112
Sirt3
Name: sirtuin 3
Synonyms: Sir2l3, 2310003L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 64384
Homologene: 81827
Btbd17
Name: BTB (POZ) domain containing 17
Synonyms: 1500005I02Rik, Tango10a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72014
Homologene: 46324
Chaserr
Name: CHD2 adjacent suppressive regulatory RNA
Synonyms: 1810026B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69170
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, GAC-1, 2900001A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106585
Homologene: 9059
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74569
Homologene: 10100
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76464
Homologene: 44890
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Ctbp1
Name: C-terminal binding protein 1
Synonyms: D4S115h, D5H4S115, BARS, D5H4S115E, CtBP1-S, CtBP1-L, CtBP3/BARS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13016
HGNC: HGNC:2494
Homologene: 1015
Txlna
Name: taxilin alpha
Synonyms: 2600010N21Rik, Txln, IL14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 109658
Homologene: 14062
Rps6ka5
Name: ribosomal protein S6 kinase, polypeptide 5
Synonyms: 6330404E13Rik, MSK1, 3110005L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73086
VEGA: 12
Homologene: 48302
Kank2
Name: KN motif and ankyrin repeat domains 2
Synonyms: Ankrd25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235041
VEGA: 9
Homologene: 9163
Tefm
Name: transcription elongation factor, mitochondrial
Synonyms: FLJ22729, 1110002N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68550
Homologene: 11657
Gpsm1
Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)
Synonyms: 1810037C22Rik, Ags3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67839
Homologene: 16987
Apex1
Name: apurinic/apyrimidinic endonuclease 1
Synonyms: Ref-1, HAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 11792
HGNC: HGNC:587
Homologene: 1241
Trmt1
Name: tRNA methyltransferase 1
Synonyms: 6720406L13Rik, D8Ertd812e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212528
Homologene: 5632
Klhl26
Name: kelch-like 26
Synonyms: C630013N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234378
Homologene: 41247
Siglece
Name: sialic acid binding Ig-like lectin E
Synonyms: mSiglec-E, Siglecl1, Siglec5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 83382
Homologene: 130669
Il16
Name: interleukin 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16170
HGNC: HGNC:5980
Homologene: 18157
Ubxn10
Name: UBX domain protein 10
Synonyms: 5730509E04Rik, Ubxd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 212190
Homologene: 17568
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244646
Homologene: 14627
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Klkb1
Name: kallikrein B, plasma 1
Synonyms: Kal-3, Kal3, Klk3, PSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16621
HGNC: HGNC:6371
Homologene: 68097
Guf1
Name: GUF1 homolog, GTPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231279
Homologene: 6505
Letm2
Name: leucine zipper-EF-hand containing transmembrane protein 2
Synonyms: D030041N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270035
Homologene: 56358
Egr4
Name: early growth response 4
Synonyms: pAT133, NGFI-C, NGF1-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13656
HGNC: HGNC:3241
Homologene: 1485
Zscan12
Name: zinc finger and SCAN domain containing 12
Synonyms: Zfp96
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22758
Homologene: 86980
Dao
Name: D-amino acid oxidase
Synonyms: DAO, Dao-1, Dao1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13142
HGNC: HGNC:2671
Homologene: 37553
Nin
Name: ninein
Synonyms: 3110068G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18080
Homologene: 40632
Ipo4
Name: importin 4
Synonyms: 8430408O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75751
Homologene: 5835
D6Ertd527e
Name: DNA segment, Chr 6, ERATO Doi 527, expressed
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 52372
Ikbke
Name: inhibitor of kappaB kinase epsilon
Synonyms: IKK-i, IKKepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56489
Homologene: 23168
Nek2
Name: NIMA (never in mitosis gene a)-related expressed kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18005
HGNC: HGNC:7745
Homologene: 74441
H2-Eb2
Name: histocompatibility 2, class II antigen E beta2
Synonyms: H-2Eb2, Ia-5, Ia5, A130038H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381091
Homologene: 128636
Snph
Name: syntaphilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241727
Homologene: 8817
Muc1
Name: mucin 1, transmembrane
Synonyms: EMA, CD227, Muc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 17829
HGNC: HGNC:7508
Homologene: 137248
Olfr1110
Name: olfactory receptor 1110
Synonyms: GA_x6K02T2Q125-48621299-48620361, MOR172-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258765
9130019O22Rik
Name: RIKEN cDNA 9130019O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78921
Homologene: 118005
Cx3cl1
Name: chemokine (C-X3-C motif) ligand 1
Synonyms: fractalkine, CX3C, neurotactin, D8Bwg0439e, Scyd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20312
Homologene: 2251
Hpgds
Name: hematopoietic prostaglandin D synthase
Synonyms: H-PGDS, Ptgds2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54486
Homologene: 113741
Coq10a
Name: coenzyme Q10A
Synonyms: LOC210582
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210582
VEGA: 10
Homologene: 27027
Snx18
Name: sorting nexin 18
Synonyms: Snag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 170625
VEGA: 13
Homologene: 14164
Gm17690
Name: predicted gene, 17690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Leprel2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Rps6-ps2
Name: ribosomal protein S6, pseudogene 2
Synonyms: Gm16406
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 675985
Gm20517
Name: predicted gene 20517
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 131,265,922 bp
  • C to T, chromosome 1 at 191,822,715 bp
  • G to A, chromosome 2 at 26,319,831 bp
  • T to C, chromosome 2 at 87,135,661 bp
  • A to G, chromosome 2 at 94,366,571 bp
  • A to C, chromosome 2 at 119,059,599 bp
  • G to A, chromosome 2 at 151,593,847 bp
  • A to T, chromosome 3 at 89,231,563 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • T to C, chromosome 4 at 129,629,599 bp
  • T to A, chromosome 4 at 138,720,876 bp
  • A to G, chromosome 5 at 25,299,876 bp
  • A to T, chromosome 5 at 33,251,013 bp
  • C to T, chromosome 5 at 69,565,493 bp
  • T to A, chromosome 5 at 114,019,926 bp
  • A to G, chromosome 6 at 65,123,634 bp
  • G to A, chromosome 6 at 85,512,340 bp
  • T to C, chromosome 6 at 87,111,317 bp
  • A to T, chromosome 6 at 124,845,568 bp
  • T to C, chromosome 6 at 128,524,040 bp
  • C to T, chromosome 6 at 139,841,681 bp
  • T to C, chromosome 7 at 43,651,505 bp
  • T to C, chromosome 7 at 73,540,874 bp
  • T to A, chromosome 7 at 83,652,325 bp
  • A to G, chromosome 7 at 127,384,745 bp
  • T to C, chromosome 7 at 140,865,000 bp
  • A to G, chromosome 8 at 25,586,699 bp
  • A to G, chromosome 8 at 45,273,575 bp
  • T to C, chromosome 8 at 70,451,544 bp
  • A to G, chromosome 8 at 84,699,149 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • A to G, chromosome 8 at 94,780,556 bp
  • A to G, chromosome 8 at 109,632,713 bp
  • G to A, chromosome 9 at 21,794,745 bp
  • A to G, chromosome 9 at 44,848,966 bp
  • A to T, chromosome 9 at 121,156,884 bp
  • T to C, chromosome 10 at 128,364,478 bp
  • A to G, chromosome 11 at 80,138,049 bp
  • A to G, chromosome 11 at 103,464,354 bp
  • C to T, chromosome 11 at 114,793,989 bp
  • A to T, chromosome 12 at 70,042,585 bp
  • C to A, chromosome 12 at 100,570,864 bp
  • C to T, chromosome 13 at 21,366,619 bp
  • T to C, chromosome 13 at 113,617,195 bp
  • T to C, chromosome 14 at 50,926,513 bp
  • G to A, chromosome 14 at 55,629,820 bp
  • T to A, chromosome 17 at 34,333,523 bp
  • T to C, chromosome 17 at 47,618,917 bp
  • T to C, chromosome 17 at 65,985,937 bp
  • T to C, chromosome 17 at 84,426,237 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4461 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041720-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.