Strain Name:
C57BL/6J-MtgxR4463Btlr/Mmmh
Stock Number:
041721-MU
Citation ID:
RRID:MMRRC_041721-MU
Other Names:
R4463 (G1), C57BL/6J-MtgxR4463Btlr
Major Collection:

Gene Information

Kifc3
Name: kinesin family member C3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16582
HGNC: HGNC:6326
Homologene: 124421
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, INK4d, p19INK4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Abcc8
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, 9030205D12Rik, A330063D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Calmbp1, Sha1, Aspm, D330028K02Rik, MCPH5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12316
Homologene: 7650
Tango6
Name: transport and golgi organization 6
Synonyms: Tango6, Tmco7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 272538
Homologene: 52121
Prmt3
Name: protein arginine N-methyltransferase 3
Synonyms: 2410018A17Rik, 2010005E20Rik, Hrmt1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71974
Homologene: 24255
Cct4
Name: chaperonin containing Tcp1, subunit 4 (delta)
Synonyms: 2610204B21Rik, A45, Cctd, T complex protein 1, delta, TCP-1 delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12464
HGNC: HGNC:1617
Homologene: 4695
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Eipr1
Name: EARP complex and GARP complex interacting protein 1
Synonyms: D12Ertd604e, Tssc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380752
VEGA: 12
Homologene: 2481
Alox12e
Name: arachidonate lipoxygenase, epidermal
Synonyms: Alox12-ps2, 8-LOX, Alox12-ps1, Aloxe, e-LOX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11685
Homologene: 105868
Fgfr4
Name: fibroblast growth factor receptor 4
Synonyms: Fgfr-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14186
HGNC: HGNC:3691
Homologene: 20461
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75619
Homologene: 8957
Olfr352
Name: olfactory receptor 352
Synonyms: GA_x6K02T2NLDC-33564136-33565083, MOR136-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258942
Homologene: 121523
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
9530053A07Rik
Name: RIKEN cDNA 9530053A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319482
Homologene: 130055
Plek
Name: pleckstrin
Synonyms: 2010300B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56193
HGNC: HGNC:9070
Homologene: 2000
Sclt1
Name: sodium channel and clathrin linker 1
Synonyms: Tg(CAG-sb10)1Dla, 2610207F23Rik, 4931421F20Rik, Tg(ACTB-sb10)1Dla, CAGGS-SB10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67161
Homologene: 27031
Ripk2
Name: receptor (TNFRSF)-interacting serine-threonine kinase 2
Synonyms: RIP2, RICK, D4Bwg0615e, CARD3, 2210420D18Rik, CCK, CARDIAK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 192656
Homologene: 37856
Capn2
Name: calpain 2
Synonyms: m-calpain, Capa-2, Capa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12334
HGNC: HGNC:1479
Homologene: 1326
Fam83a
Name: family with sequence similarity 83, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239463
Homologene: 13158
Olfr1189
Name: olfactory receptor 1189
Synonyms: GA_x6K02T2Q125-50079044-50079964, MOR237-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258768
Homologene: 81569
Vstm4
Name: V-set and transmembrane domain containing 4
Synonyms: E130203B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 320736
Homologene: 18707
Itgbl1
Name: integrin, beta-like 1
Synonyms: B930011D01Rik, with EGF-like repeat domains
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223272
HGNC: HGNC:6164
Homologene: 3519
Mctp1
Name: multiple C2 domains, transmembrane 1
Synonyms: 2810465F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78771
Homologene: 75211
Cyp3a57
Name: cytochrome P450, family 3, subfamily a, polypeptide 57
Synonyms: EG622127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622127
Homologene: 135775
Olfr1340
Name: olfactory receptor 1340
Synonyms: MOR258-6, GA_x6K02T2QD9B-18823451-18822504
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258301
Homologene: 122779
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11980
Homologene: 48402
Ccdc158
Name: coiled-coil domain containing 158
Synonyms: 4932413O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320696
Homologene: 18560
Kbtbd3
Name: kelch repeat and BTB (POZ) domain containing 3
Synonyms: Bklhd3, 2200003A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69149
Homologene: 12300
Slc2a4
Name: solute carrier family 2 (facilitated glucose transporter), member 4
Synonyms: twgy, Glut-4, Glut4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20528
Homologene: 74381
Idi1
Name: isopentenyl-diphosphate delta isomerase
Synonyms: 4832416K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319554
HGNC: HGNC:5387
Homologene: 3315
Chst8
Name: carbohydrate sulfotransferase 8
Synonyms: 1500011J21Rik, GalNAc4ST-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68947
Homologene: 41483
Xylb
Name: xylulokinase homolog (H. influenzae)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102448
VEGA: 9
Homologene: 3746
Stpg4
Name: sperm tail PG rich repeat containing 4
Synonyms: 1700011E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75467
Homologene: 18339
Hectd2
Name: HECT domain E3 ubiquitin protein ligase 2
Synonyms: A630025O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226098
Homologene: 6280
Vmn1r196
Name: vomeronasal 1 receptor 196
Synonyms: V1rh19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100312484
Homologene: 110880
Abcb1a
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1A
Synonyms: MDR3, Pgp, Pgy-3, P-glycoprotein, Pgy3, mdr-3, Mdr1a, multiple drug resistant 1a, Evi32, P-gp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18671
HGNC: HGNC:40
Homologene: 55496
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Psg27
Name: pregnancy-specific glycoprotein 27
Synonyms: cea15, EG545925
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545925
Homologene: 110989
Pp2d1
Name: protein phosphatase 2C-like domain containing 1
Synonyms: 4921523A10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110332
VEGA: 17
Homologene: 16981
Angel1
Name: angel homolog 1
Synonyms: 1110030H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68737
Homologene: 32251
Sectm1a
Name: secreted and transmembrane 1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 209588
Homologene: 134112
Snph
Name: syntaphilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241727
Homologene: 8817
Cyp4f37
Name: cytochrome P450, family 4, subfamily f, polypeptide 37
Synonyms: Gm9705
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 677156
VEGA: 17
Homologene: 135840
Phka1
Name: phosphorylase kinase alpha 1
Synonyms: Phka, 9830108K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18679
HGNC: HGNC:8925
Homologene: 1981
Myzap
Name: myocardial zonula adherens protein
Synonyms: Gcom1, Grinl1a7, Myozap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102371
Homologene: 134156
Zc2hc1c
Name: zinc finger, C2HC-type containing 1C
Synonyms: Fam164c, 2810002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 72350
Homologene: 23461
Gatad1
Name: GATA zinc finger domain containing 1
Synonyms: 2310031E19Rik, Odag, 2810047M21Rik, 9130430G15Rik, B330017N08Rik, 8430439A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67210
Homologene: 56916
1700084C06Rik
Name: RIKEN cDNA 1700084C06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Gm1330
Name: predicted gene 1330
Synonyms: LOC383753
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Gm6189
Name: predicted gene 6189
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 620899
Gm8055
Name: predicted pseudogene 8055
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 666342
RP23-474A1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Gm9990
Name: predicted gene 9990
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Larp6
Name: La ribonucleoprotein domain family, member 6
Synonyms: acheron, Achn, 5430431G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67557
Homologene: 32408
Gm2056
Name: predicted gene 2056
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100039129
VEGA: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 63,735,809 bp
  • T to C, chromosome 1 at 80,383,186 bp
  • T to C, chromosome 1 at 118,836,008 bp
  • T to A, chromosome 1 at 139,455,010 bp
  • G to A, chromosome 1 at 177,937,713 bp
  • G to A, chromosome 1 at 182,479,764 bp
  • T to C, chromosome 2 at 36,870,193 bp
  • GCC to GC, chromosome 2 at 52,279,722 bp
  • T to C, chromosome 2 at 88,592,632 bp
  • A to T, chromosome 2 at 149,003,144 bp
  • A to T, chromosome 2 at 151,594,115 bp
  • C to T, chromosome 3 at 41,671,487 bp
  • A to C, chromosome 3 at 127,684,348 bp
  • T to C, chromosome 4 at 16,151,968 bp
  • T to C, chromosome 4 at 118,726,658 bp
  • G to T, chromosome 5 at 3,647,404 bp
  • T to C, chromosome 5 at 8,719,981 bp
  • T to C, chromosome 5 at 67,767,147 bp
  • T to C, chromosome 5 at 92,634,300 bp
  • A to T, chromosome 5 at 145,381,274 bp
  • G to T, chromosome 7 at 18,557,085 bp
  • A to G, chromosome 7 at 28,150,719 bp
  • T to C, chromosome 7 at 34,675,220 bp
  • T to C, chromosome 7 at 46,106,581 bp
  • T to C, chromosome 7 at 49,818,089 bp
  • T to C, chromosome 7 at 97,720,949 bp
  • T to A, chromosome 8 at 90,978,999 bp
  • T to C, chromosome 8 at 95,102,116 bp
  • A to T, chromosome 8 at 106,689,074 bp
  • G to A, chromosome 9 at 4,331,257 bp
  • T to C, chromosome 9 at 21,091,827 bp
  • C to G, chromosome 9 at 21,290,889 bp
  • C to A, chromosome 9 at 60,736,996 bp
  • G to T, chromosome 9 at 71,555,651 bp
  • A to G, chromosome 9 at 119,386,367 bp
  • A to G, chromosome 10 at 40,012,824 bp
  • T to C, chromosome 11 at 16,981,873 bp
  • T to C, chromosome 11 at 23,001,357 bp
  • G to A, chromosome 11 at 69,943,322 bp
  • A to G, chromosome 11 at 70,318,256 bp
  • T to A, chromosome 11 at 121,069,651 bp
  • G to A, chromosome 12 at 28,859,339 bp
  • A to G, chromosome 12 at 85,290,297 bp
  • A to T, chromosome 12 at 86,705,290 bp
  • A to G, chromosome 12 at 88,027,359 bp
  • G to A, chromosome 13 at 8,887,472 bp
  • A to G, chromosome 13 at 22,293,683 bp
  • G to A, chromosome 13 at 55,156,467 bp
  • A to T, chromosome 13 at 76,712,087 bp
  • T to A, chromosome 14 at 32,917,876 bp
  • C to T, chromosome 14 at 123,840,668 bp
  • T to C, chromosome 15 at 57,995,259 bp
  • A to G, chromosome 17 at 32,627,736 bp
  • A to G, chromosome 17 at 53,515,858 bp
  • G to A, chromosome 17 at 67,761,700 bp
  • A to G, chromosome 17 at 87,389,673 bp
  • T to C, chromosome 19 at 36,595,209 bp
  • A to G, chromosome X at 102,545,384 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4463 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041721-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.