Strain Name:
C57BL/6J-MtgxR4464Btlr/Mmmh
Stock Number:
041722-MU
Citation ID:
RRID:MMRRC_041722-MU
Other Names:
R4464 (G1), C57BL/6J-MtgxR4464Btlr
Major Collection:

Gene Information

Mbd4
Name: methyl-CpG binding domain protein 4
Synonyms: Med1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17193
HGNC: HGNC:6919
Homologene: 2916
Kifap3
Name: kinesin-associated protein 3
Synonyms: KAP3, Smg GDS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16579
Homologene: 7799
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: C130052G03Rik, LOC237877
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237877
Homologene: 32611
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 104831
Homologene: 135706
Stat1
Name: signal transducer and activator of transcription 1
Synonyms: 2010005J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20846
Homologene: 21428
Trim66
Name: tripartite motif-containing 66
Synonyms: D7H11orf29, Tif1d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 330627
Homologene: 28044
Thbs1
Name: thrombospondin 1
Synonyms: TSP-1, tbsp1, TSP1, Thbs-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 21825
Homologene: 31142
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Rb1
Name: RB transcriptional corepressor 1
Synonyms: retinoblastoma 1, Rb-1, pRb, Rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 19645
HGNC: HGNC:9884
Homologene: 272
Dennd1a
Name: DENN/MADD domain containing 1A
Synonyms: 6030446I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227801
Homologene: 17141
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74499
Homologene: 11542
Zfp429
Name: zinc finger protein 429
Synonyms: 2810487A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72807
HGNC: null
Homologene: 110878
Armc3
Name: armadillo repeat containing 3
Synonyms: 4921513G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70882
Homologene: 31589
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 338370
VEGA: 14
Homologene: 21832
Ifngr1
Name: interferon gamma receptor 1
Synonyms: IFN-gamma R, IFN-gammaR, Ifngr, Ifgr, Nktar, CD119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15979
HGNC: HGNC:5439
Homologene: 359
Acot10
Name: acyl-CoA thioesterase 10
Synonyms: MT-ACT48, Acate3, p48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 64833
VEGA: 15
Homologene: 8206
Olfr109
Name: olfactory receptor 109
Synonyms: MOR250-1, GA_x6K02T2PSCP-1914078-1915022
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258832
Homologene: 12811
Krt86
Name: keratin 86
Synonyms: Krt2-10, MHb4, Krt2-11, Khb4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 16679
VEGA: 15
HGNC: HGNC:6463
Homologene: 1717
Gpr158
Name: G protein-coupled receptor 158
Synonyms: 5330427M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241263
Homologene: 19381
Slc34a2
Name: solute carrier family 34 (sodium phosphate), member 2
Synonyms: Npt2b, D5Ertd227e, type IIb Na/Picotransporter, NaPi-2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20531
Homologene: 2297
Aldh8a1
Name: aldehyde dehydrogenase 8 family, member A1
Synonyms: RALDH4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237320
Homologene: 23369
Tkt
Name: transketolase
Synonyms: p68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21881
Homologene: 68154
Gm7535
Name: predicted gene 7535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 665187
VEGA: 17
HGNC: null
Homologene: null
Abcc3
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
Synonyms: MRP3, 1700019L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76408
HGNC: HGNC:54
Homologene: 68364
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76261
Homologene: 49537
Rad51ap1
Name: RAD51 associated protein 1
Synonyms: RAB22, 2510006L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 19362
Homologene: 48436
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Lrrcc1
Name: leucine rich repeat and coiled-coil domain containing 1
Synonyms: 4932441F23Rik, 1200008A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71710
Homologene: 12559
St3gal2
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Synonyms: ST3GalII, Siat5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20444
Homologene: 5047
Cst12
Name: cystatin 12
Synonyms: cystatin TE-1, 1700006F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 69362
Homologene: 17808
Psg29
Name: pregnancy-specific glycoprotein 29
Synonyms: cea17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 114872
HGNC: null
Homologene: 83380
Asnsd1
Name: asparagine synthetase domain containing 1
Synonyms: 2210409M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70396
Homologene: 6564
Prxl2a
Name: peroxiredoxin like 2A
Synonyms: Fam213a, 5730469M10Rik, Adiporedoxin, Adrx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 70564
Homologene: 12356
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 52,137,416 bp
  • C to A, chromosome 1 at 53,352,527 bp
  • C to A, chromosome 1 at 163,817,895 bp
  • T to C, chromosome 2 at 19,248,659 bp
  • T to A, chromosome 2 at 21,826,999 bp
  • A to T, chromosome 2 at 38,243,390 bp
  • A to G, chromosome 2 at 118,122,980 bp
  • G to A, chromosome 2 at 148,789,517 bp
  • A to G, chromosome 3 at 14,557,318 bp
  • C to G, chromosome 4 at 51,229,651 bp
  • T to C, chromosome 5 at 53,069,182 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • A to G, chromosome 6 at 85,620,021 bp
  • A to G, chromosome 6 at 115,849,502 bp
  • T to C, chromosome 6 at 126,934,768 bp
  • A to T, chromosome 7 at 17,210,650 bp
  • A to T, chromosome 7 at 109,477,690 bp
  • A to G, chromosome 8 at 110,967,502 bp
  • G to A, chromosome 9 at 110,386,813 bp
  • G to A, chromosome 10 at 19,597,517 bp
  • C to A, chromosome 10 at 21,388,941 bp
  • T to A, chromosome 11 at 80,100,311 bp
  • A to G, chromosome 11 at 94,358,786 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • T to C, chromosome 13 at 67,390,498 bp
  • A to G, chromosome 14 at 30,568,274 bp
  • T to A, chromosome 14 at 40,997,875 bp
  • C to A, chromosome 14 at 73,199,198 bp
  • T to C, chromosome 14 at 123,323,350 bp
  • G to A, chromosome 15 at 20,665,744 bp
  • G to A, chromosome 15 at 101,473,914 bp
  • C to A, chromosome 17 at 17,911,662 bp
  • C to T, chromosome 17 at 37,466,851 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4464 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041722-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.