Strain Name:
C57BL/6J-MtgxR4473Btlr/Mmmh
Stock Number:
041730-MU
Citation ID:
RRID:MMRRC_041730-MU
Other Names:
R4473 (G1), C57BL/6J-MtgxR4473Btlr
Major Collection:

Gene Information

Tfpi
Name: tissue factor pathway inhibitor
Synonyms: A630013F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21788
Homologene: 4579
Acot7
Name: acyl-CoA thioesterase 7
Synonyms: 2410041A17Rik, Bach, AU014716
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70025
Homologene: 15780
Elavl2
Name: ELAV like RNA binding protein 1
Synonyms: mel-N1, Hub
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15569
HGNC: HGNC:3313
Homologene: 20930
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: sarco/endoplasmic reticulum Ca2+-ATPase 2, SERCA2, 9530097L16Rik, D5Wsu150e, SERCA2B, Serca2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Ppp6r3
Name: protein phosphatase 6, regulatory subunit 3
Synonyms: 9130026N02Rik, D19Bwg1430e, 4930528G08Rik, D19Ertd703e, Pp6r3, Pptcs3, Saps3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52036
VEGA: 19
HGNC: HGNC:1173
Homologene: 115911
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Trim66
Name: tripartite motif-containing 66
Synonyms: D7H11orf29, Tif1d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330627
Homologene: 28044
Ifnar1
Name: interferon (alpha and beta) receptor 1
Synonyms: Ifrc, Ifar, IFN-alpha/betaR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 15975
HGNC: HGNC:5432
Homologene: 524
Klhl23
Name: kelch-like 23
Synonyms: C130068N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277396
Homologene: 18628
Alb
Name: albumin
Synonyms: Alb-1, Alb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11657
HGNC: HGNC:399
Homologene: 405
Rab22a
Name: RAB22A, member RAS oncogene family
Synonyms: E130120E14Rik, 3732413A17Rik, Rab22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19334
HGNC: HGNC:9764
Homologene: 10782
Mthfd2
Name: methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase
Synonyms: NMDMC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17768
HGNC: HGNC:7434
Homologene: 21321
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Corl2, Gm7348
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75619
Homologene: 8957
Vnn1
Name: vanin 1
Synonyms: pantetheinase, V-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22361
Homologene: 32130
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
D630003M21Rik
Name: RIKEN cDNA D630003M21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228846
Homologene: 18598
Spata20
Name: spermatogenesis associated 20
Synonyms: Tisp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217116
Homologene: 11264
Dcaf7
Name: DDB1 and CUL4 associated factor 7
Synonyms: 2610037L01Rik, 1700012F10Rik, Wdr68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71833
Homologene: 55930
Olfr1161
Name: olfactory receptor 1161
Synonyms: GA_x6K02T2Q125-49516664-49517629, MOR174-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258845
Homologene: 138318
Fmo1
Name: flavin containing monooxygenase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14261
HGNC: HGNC:3769
Homologene: 55520
Vmn1r5
Name: vomeronasal 1 receptor 5
Synonyms: V1rc19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171192
Homologene: 128340
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74682
Homologene: 10814
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Fancf
Name: Fanconi anemia, complementation group F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100040608
HGNC: HGNC:3587
Homologene: 75185
Ttll1
Name: tubulin tyrosine ligase-like 1
Synonyms: 6330444E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319953
HGNC: HGNC:1312
Homologene: 8174
Actg1
Name: actin, gamma, cytoplasmic 1
Synonyms: E51, Actl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11465
HGNC: HGNC:144
Homologene: 74402
Podnl1
Name: podocan-like 1
Synonyms: 5832418A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244550
Homologene: 41590
Eddm3b
Name: epididymal protein 3B
Synonyms: Fam12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219026
VEGA: 14
Homologene: 75184
Sox18
Name: SRY (sex determining region Y)-box 18
Synonyms: Sry-related HMG-box gene 18, Ragl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20672
Homologene: 7546
Pramel38
Name: PRAME like 38
Synonyms: Gm3106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041032
Homologene: 133100
3110021A11Rik
Name: RIKEN cDNA 3110021A11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67289
VEGA: 6
Ighv1-49
Name: immunoglobulin heavy variable 1-49
Synonyms: Gm16830
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629925
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,731,674 bp
  • A to G, chromosome 1 at 162,850,163 bp
  • A to G, chromosome 2 at 69,823,807 bp
  • A to T, chromosome 2 at 84,458,082 bp
  • T to A, chromosome 2 at 88,025,120 bp
  • G to A, chromosome 2 at 158,213,462 bp
  • C to T, chromosome 2 at 173,695,263 bp
  • T to C, chromosome 2 at 181,670,876 bp
  • T to C, chromosome 3 at 127,680,018 bp
  • T to C, chromosome 4 at 91,261,009 bp
  • C to A, chromosome 4 at 152,206,856 bp
  • T to C, chromosome 5 at 72,339,057 bp
  • T to C, chromosome 5 at 90,464,053 bp
  • A to G, chromosome 5 at 94,218,170 bp
  • A to T, chromosome 5 at 122,457,264 bp
  • G to A, chromosome 6 at 56,985,648 bp
  • A to G, chromosome 6 at 83,310,535 bp
  • G to T, chromosome 6 at 119,848,456 bp
  • A to G, chromosome 7 at 51,862,200 bp
  • A to T, chromosome 7 at 109,481,995 bp
  • A to T, chromosome 8 at 84,131,985 bp
  • T to C, chromosome 10 at 23,894,891 bp
  • A to G, chromosome 11 at 94,483,089 bp
  • C to T, chromosome 11 at 106,046,750 bp
  • G to A, chromosome 11 at 120,348,259 bp
  • A to G, chromosome 12 at 9,015,995 bp
  • A to T, chromosome 12 at 115,055,339 bp
  • A to G, chromosome 14 at 51,116,779 bp
  • T to A, chromosome 15 at 83,492,609 bp
  • A to G, chromosome 16 at 13,664,685 bp
  • T to C, chromosome 16 at 91,495,170 bp
  • C to T, chromosome 18 at 76,859,461 bp
  • G to T, chromosome 19 at 3,511,978 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4473 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041730-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.