Strain Name:
C57BL/6J-MtgxR4475Btlr/Mmmh
Stock Number:
041732-MU
Citation ID:
RRID:MMRRC_041732-MU
Other Names:
R4475 (G1), C57BL/6J-MtgxR4475Btlr
Major Collection:

Strain Information

Esr2
Name: estrogen receptor 2 (beta)
Synonyms: ER beta, ERbeta, oestrogen receptor beta, Estrb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13983
HGNC: HGNC:3468
Homologene: 1100
Ighm
Name: immunoglobulin heavy constant mu
Synonyms: Ig mu, Igh6, Igh-M, muH, IgM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16019
HGNC: HGNC:5541
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Dlx5
Name: distal-less homeobox 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13395
HGNC: HGNC:2918
Homologene: 3825
Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
Hells
Name: helicase, lymphoid specific
Synonyms: Lysh, proliferation-associated SNF2-like, PASG, LSH, YFK8, E130115I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 15201
HGNC: HGNC:4861
Homologene: 50037
Dgkh
Name: diacylglycerol kinase, eta
Synonyms: 5930402B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380921
VEGA: 14
HGNC: HGNC:2854
Homologene: 99373
Tnip1
Name: TNFAIP3 interacting protein 1
Synonyms: A20-binding inhibitor of NF-kappa B activation, ABIN1, VAN, Nef
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57783
Homologene: 31355
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Usp34
Name: ubiquitin specific peptidase 34
Synonyms: A530081C03Rik, Murr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17847
Homologene: 40978
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Atp6v1c1
Name: ATPase, H+ transporting, lysosomal V1 subunit C1
Synonyms: 1700025B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66335
VEGA: 15
HGNC: HGNC:856
Homologene: 1281
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Adgra3
Name: adhesion G protein-coupled receptor A3
Synonyms: 3830613O22Rik, Tem5-like, Gpr125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70693
Homologene: 19235
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Srpr
Name: signal recognition particle receptor ('docking protein')
Synonyms: 1300011P19Rik, D11Mgi27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67398
Homologene: 2364
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: LOC381605, PARIS-1, PARIS1, A630005A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381605
Homologene: 10190
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: 9430028L06Rik, 9030411M15Rik, Fam38b2, Piezo2, Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 667742
Homologene: 49695
Sim2
Name: single-minded family bHLH transcription factor 2
Synonyms: bHLHe15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20465
VEGA: 16
Homologene: 3716
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Plek
Name: pleckstrin
Synonyms: 2010300B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56193
HGNC: HGNC:9070
Homologene: 2000
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: MSF, DOL54, lubricin, SZP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: 1110032O15Rik, AceCS2, Acas2, Acas2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68738
Homologene: 56037
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), neurexin I beta, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, 1700062G21Rik, A230068P09Rik, 9330127H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Rrs1
Name: ribosome biogenesis regulator 1
Synonyms: 5730466A07Rik, D1Ertd701e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59014
Homologene: 41014
Oprk1
Name: opioid receptor, kappa 1
Synonyms: KOR-1, R21, Oprk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18387
HGNC: HGNC:8154
Homologene: 20253
Zfp365
Name: zinc finger protein 365
Synonyms: Su48, DBZ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216049
Homologene: 8975
Or10j2
Name: olfactory receptor family 10 subfamily J member 2
Synonyms: GA_x6K02T2P20D-20826777-20827719, GA_x6K02T2R7CC-581296-580364, MOR267-12P, MOR267-8, Olfr1403, Olfr418-ps1, Olfr418
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258645
HGNC: HGNC:8175
Homologene: 8218
A630010A05Rik
Name: RIKEN cDNA A630010A05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545280
Hspa8
Name: heat shock protein 8
Synonyms: Hsp73, Hsc71, Hsc70, 2410008N15Rik, Hsc73
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15481
HGNC: HGNC:5241
Homologene: 68524
Trim3
Name: tripartite motif-containing 3
Synonyms: BERP1, HAC1, Rnf22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 55992
Homologene: 21290
Aga
Name: aspartylglucosaminidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11593
HGNC: HGNC:318
Homologene: 13
Or9s27
Name: olfactory receptor family 9 subfamily S member 27
Synonyms: GA_x6K02T2R7CC-81165686-81164721, MOR208-4, Olfr1412
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258274
Homologene: 115514
Smpd5
Name: sphingomyelin phosphodiesterase 5
Synonyms: Gm10345
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100503915
Homologene: 132205
Vmn2r129
Name: vomeronasal 2, receptor 129
Synonyms: V2r8, V2r9, Vmn2r-ps160, Vmn2r-ps159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22314
Tmem52b
Name: transmembrane protein 52B
Synonyms: D630042F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330428
Homologene: 27407
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 5,602,601 bp
  • T to C, chromosome 1 at 9,545,585 bp
  • T to C, chromosome 1 at 92,588,579 bp
  • T to C, chromosome 1 at 150,454,859 bp
  • T to C, chromosome 1 at 173,270,913 bp
  • T to A, chromosome 2 at 27,180,972 bp
  • G to A, chromosome 2 at 150,637,913 bp
  • A to G, chromosome 3 at 37,040,395 bp
  • A to G, chromosome 4 at 46,609,080 bp
  • T to G, chromosome 4 at 156,338,790 bp
  • A to T, chromosome 5 at 50,001,898 bp
  • T to C, chromosome 6 at 6,881,663 bp
  • C to T, chromosome 6 at 129,514,256 bp
  • A to G, chromosome 7 at 105,617,802 bp
  • T to C, chromosome 8 at 53,511,836 bp
  • A to G, chromosome 9 at 35,212,859 bp
  • T to A, chromosome 9 at 40,804,146 bp
  • C to T, chromosome 9 at 72,671,239 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • C to A, chromosome 10 at 67,888,920 bp
  • C to T, chromosome 11 at 16,985,528 bp
  • T to C, chromosome 11 at 23,457,975 bp
  • A to G, chromosome 11 at 54,939,596 bp
  • T to C, chromosome 12 at 53,141,643 bp
  • C to T, chromosome 12 at 76,133,942 bp
  • A to G, chromosome 12 at 113,420,893 bp
  • T to C, chromosome 14 at 78,589,878 bp
  • T to C, chromosome 15 at 38,677,573 bp
  • T to C, chromosome 15 at 76,294,726 bp
  • A to G, chromosome 16 at 13,664,685 bp
  • T to A, chromosome 16 at 14,589,363 bp
  • T to C, chromosome 16 at 94,125,791 bp
  • T to C, chromosome 17 at 30,656,985 bp
  • G to T, chromosome 17 at 90,701,982 bp
  • A to G, chromosome 18 at 63,102,099 bp
  • A to C, chromosome 18 at 77,948,508 bp
  • A to G, chromosome 19 at 38,945,529 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4475 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041732-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.