Strain Name:
C57BL/6J-MtgxR4478Btlr/Mmmh
Stock Number:
041735-MU
Citation ID:
RRID:MMRRC_041735-MU
Other Names:
R4478 (G1), C57BL/6J-MtgxR4478Btlr
Major Collection:

Gene Information

Ubl3
Name: ubiquitin-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 24109
Homologene: 5153
Wdr73
Name: WD repeat domain 73
Synonyms: 2410008B13Rik, 1200011I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71968
Homologene: 41899
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: AD013, A330063D19Rik, 9030205D12Rik, 1810014J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: D030060O14Rik, Rab11-FIP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 215445
Homologene: 49396
Sdad1
Name: SDA1 domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231452
Homologene: 6036
Tmem87a
Name: transmembrane protein 87A
Synonyms: A930025J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211499
Homologene: 9165
Myo9b
Name: myosin IXb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Fnbp1
Name: formin binding protein 1
Synonyms: FBP1, 1110057E06Rik, 2210010H06Rik, FBP17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14269
Homologene: 100983
Zfp1
Name: zinc finger protein 1
Synonyms: mkr-1, Zfp-1, Fnp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22640
Homologene: 32132
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, D630001M17Rik, 9330188B09Rik, LOC381026, 4921513O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Eif4g1
Name: eukaryotic translation initiation factor 4, gamma 1
Synonyms: E030015G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208643
HGNC: HGNC:3296
Homologene: 110725
Dag1
Name: dystroglycan 1
Synonyms: D9Wsu13e, dystrophin associated glycoprotein 1, beta-dystroglycan, alpha-dystroglycan, DG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13138
HGNC: HGNC:2666
Homologene: 3234
Snap29
Name: synaptosomal-associated protein 29
Synonyms: 1300018G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67474
Homologene: 3512
Ank1
Name: ankyrin 1, erythroid
Synonyms: pale, Ank-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Cpne5
Name: copine V
Synonyms: A830083G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240058
HGNC: HGNC:2318
Homologene: 23266
Chp2
Name: calcineurin-like EF hand protein 2
Synonyms: 2010110P09Rik, Cbhp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70261
Homologene: 11131
Tnr
Name: tenascin R
Synonyms: J1-tenascin, janusin, restrictin, TN-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21960
Homologene: 124416
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101095
Homologene: 2647
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 1300010F03Rik, 4932416F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219189
VEGA: 14
Homologene: 44881
Slc39a12
Name: solute carrier family 39 (zinc transporter), member 12
Synonyms: LOC277468
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277468
Homologene: 17654
Stard7
Name: START domain containing 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99138
Homologene: 32463
Olfr1100
Name: olfactory receptor 1100
Synonyms: GA_x6K02T2Q125-48465387-48464422, MOR206-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258587
Homologene: 34954
Il6ra
Name: interleukin 6 receptor, alpha
Synonyms: Il6r, IL-6 receptor alpha chain, CD126, IL-6R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16194
HGNC: HGNC:6019
Homologene: 474
Vmn2r9
Name: vomeronasal 2, receptor 9
Synonyms: EG435864
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 435864
Homologene: 129606
Hid1
Name: HID1 domain containing
Synonyms: C630004H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217310
Homologene: 12737
D130040H23Rik
Name: RIKEN cDNA D130040H23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 211135
Homologene: 138403
Ap1m2
Name: adaptor protein complex AP-1, mu 2 subunit
Synonyms: D9Ertd818e, [m]1B, mu1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11768
VEGA: 9
HGNC: HGNC:558
Homologene: 55906
Zfp616
Name: zinc finger protein 616
Synonyms: Gm12330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327963
Homologene: 88945
Mrc2
Name: mannose receptor, C type 2
Synonyms: novel lectin, uPARAP, Endo180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: Vmn2r68-ps, EG620697
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Stat5b
Name: signal transducer and activator of transcription 5B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20851
Homologene: 55718
Cdh15
Name: cadherin 15
Synonyms: M cadherin, Cdh14, Mcad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Kndc1
Name: kinase non-catalytic C-lobe domain (KIND) containing 1
Synonyms: very-kind, VKIND, B830014K08Rik, 2410012C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76484
Homologene: 45138
Ptpn22
Name: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
Synonyms: Ptpn8, 70zpep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19260
HGNC: HGNC:9652
Homologene: 7498
Adam3
Name: a disintegrin and metallopeptidase domain 3 (cyritestin)
Synonyms: tMDC, Taz83, Taz83, Cyrn1, ADAM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Spef1l
Name: sperm flagellar 1 like
Synonyms: 6430531B16Rik, LOC381933
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381933
Homologene: 134514
Gm20498
Name: predicted gene 20498
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 105940408
Olfr433
Name: olfactory receptor 433
Synonyms: MOR123-1, GA_x6K02T2P20D-21133014-21132070
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258712
Homologene: 133588
Tgfb2
Name: transforming growth factor, beta 2
Synonyms: Tgf-beta2, Tgfb-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21808
Homologene: 2432
Kcnk18
Name: potassium channel, subfamily K, member 18
Synonyms: Tresk-2, LOC332396, Tresk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 332396
VEGA: 19
Homologene: 133808
Fabp9
Name: fatty acid binding protein 9, testis
Synonyms: 1700007P10Rik, Tlbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 21884
HGNC: HGNC:3563
Homologene: 40776
Olfr779
Name: olfactory receptor 779
Synonyms: GA_x6K02T2PULF-10991899-10992843, Gm9792, MOR110-11P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 544749
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 159,884,756 bp
  • C to T, chromosome 1 at 174,042,616 bp
  • A to G, chromosome 1 at 186,632,499 bp
  • T to A, chromosome 2 at 14,420,179 bp
  • G to A, chromosome 2 at 31,105,254 bp
  • G to T, chromosome 2 at 86,978,218 bp
  • C to T, chromosome 2 at 120,369,343 bp
  • T to A, chromosome 2 at 127,284,259 bp
  • T to C, chromosome 3 at 10,197,106 bp
  • T to A, chromosome 3 at 89,890,290 bp
  • T to C, chromosome 3 at 103,902,064 bp
  • A to G, chromosome 5 at 92,297,160 bp
  • T to C, chromosome 5 at 108,846,277 bp
  • C to T, chromosome 5 at 148,511,977 bp
  • A to T, chromosome 6 at 32,196,133 bp
  • A to T, chromosome 6 at 47,890,696 bp
  • T to C, chromosome 7 at 80,893,221 bp
  • TCC to TC, chromosome 7 at 85,221,550 bp
  • T to G, chromosome 7 at 120,071,863 bp
  • A to G, chromosome 7 at 122,220,918 bp
  • A to T, chromosome 7 at 139,920,684 bp
  • A to T, chromosome 7 at 139,975,860 bp
  • C to T, chromosome 8 at 23,120,578 bp
  • T to C, chromosome 8 at 24,695,155 bp
  • C to A, chromosome 8 at 69,302,503 bp
  • A to T, chromosome 8 at 71,291,081 bp
  • T to C, chromosome 8 at 91,034,031 bp
  • A to G, chromosome 8 at 111,670,543 bp
  • A to G, chromosome 8 at 122,864,676 bp
  • A to G, chromosome 9 at 21,298,213 bp
  • G to C, chromosome 9 at 108,208,730 bp
  • C to T, chromosome 10 at 129,311,776 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to G, chromosome 11 at 74,083,170 bp
  • A to G, chromosome 11 at 100,787,284 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • G to A, chromosome 11 at 115,361,655 bp
  • T to C, chromosome 12 at 81,489,216 bp
  • T to A, chromosome 14 at 78,868,801 bp
  • G to A, chromosome 15 at 91,723,188 bp
  • T to C, chromosome 16 at 17,428,155 bp
  • G to T, chromosome 16 at 20,678,843 bp
  • A to T, chromosome 16 at 21,545,236 bp
  • C to A, chromosome 16 at 74,015,873 bp
  • C to T, chromosome 17 at 26,016,083 bp
  • T to C, chromosome 17 at 29,209,476 bp
  • T to C, chromosome 19 at 59,235,244 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4478 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041735-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.