Strain Name:
C57BL/6J-MtgxR4479Btlr/Mmmh
Stock Number:
041736-MU
Citation ID:
RRID:MMRRC_041736-MU
Other Names:
R4479 (G1), C57BL/6J-MtgxR4479Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 54445
Homologene: 41325
Wdr73
Name: WD repeat domain 73
Synonyms: 1200011I23Rik, 2410008B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71968
Homologene: 41899
Tti1
Name: TELO2 interacting protein 1
Synonyms: 2610036D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75425
Homologene: 40969
Ints4
Name: integrator complex subunit 4
Synonyms: 2610034N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101861
Homologene: 69427
Psma3
Name: proteasome subunit alpha 3
Synonyms: Lmpc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19167
HGNC: HGNC:9532
Homologene: 2082
Lrrc28
Name: leucine rich repeat containing 28
Synonyms: 2310058O11Rik, 2210012C09Rik, 1300004K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67867
Homologene: 16944
Zkscan5
Name: zinc finger with KRAB and SCAN domains 5
Synonyms: hKraba1, Zfp95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22757
Homologene: 8734
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Eif4g1
Name: eukaryotic translation initiation factor 4, gamma 1
Synonyms: E030015G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208643
HGNC: HGNC:3296
Homologene: 110725
Chp2
Name: calcineurin-like EF hand protein 2
Synonyms: Cbhp2, 2010110P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70261
Homologene: 11131
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Irs1
Name: insulin receptor substrate 1
Synonyms: IRS-1, G972R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Abcc6
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
Synonyms: DCC, Mrp6, Dyscalc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27421
HGNC: HGNC:57
Homologene: 55559
Osgepl1
Name: O-sialoglycoprotein endopeptidase-like 1
Synonyms: MGC13061, 2610001M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72085
Homologene: 5561
Olfr1243
Name: olfactory receptor 1243
Synonyms: GA_x6K02T2Q125-50972538-50971621, MOR231-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258971
Homologene: 27317
D130040H23Rik
Name: RIKEN cDNA D130040H23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 211135
Homologene: 138403
Erlin2
Name: ER lipid raft associated 2
Synonyms: Spfh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244373
HGNC: HGNC:1356
Homologene: 5193
Usp43
Name: ubiquitin specific peptidase 43
Synonyms: C630032K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216835
Homologene: 129879
Zfp286
Name: zinc finger protein 286
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192651
Homologene: 56892
Aldh1a3
Name: aldehyde dehydrogenase family 1, subfamily A3
Synonyms: ALDH6, V1, retinaldehyde dehydrogenase 3, RALDH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56847
HGNC: HGNC:409
Homologene: 68080
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Psg18
Name: pregnancy specific glycoprotein 18
Synonyms: mmCGM6, Cea-3, Cea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26438
Homologene: 110989
Tas2r115
Name: taste receptor, type 2, member 115
Synonyms: Tas2r15, mGR15, mt2r49, T2R15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 353325
Slc7a11
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
Synonyms: System xc, xc, xCT, sut, 9930009M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26570
Homologene: 22684
Dusp15
Name: dual specificity phosphatase-like 15
Synonyms: T-DSP10, LMW-DSP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 252864
Homologene: 33586
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: LOC380650, C030032C09Rik, E530015N03Rik, AIDA-1b, Gm10937
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77531
Homologene: 51570
Olfr206
Name: olfactory receptor 206
Synonyms: GA_x54KRFPKG5P-55560552-55559632, MOR182-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258993
Homologene: 74262
Gm3159
Name: predicted gene 3159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100041139
Homologene: 115686
Olfr1420
Name: olfactory receptor 1420
Synonyms: GA_x6K02T2RE5P-2247227-2248156, MOR266-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258405
Homologene: 17234
F830104G03Rik
Name: RIKEN cDNA F830104G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Zbp1
Name: Z-DNA binding protein 1
Synonyms: 2010010H03Rik, mZaDLM, Dai
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 58203
Homologene: 10972
Gm14252
Name: predicted gene 14252
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Ighv1-22
Name: immunoglobulin heavy variable 1-22
Synonyms: Gm16860
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780883
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,321,318 bp
  • G to A, chromosome 1 at 82,287,294 bp
  • A to G, chromosome 2 at 89,528,170 bp
  • A to G, chromosome 2 at 152,944,182 bp
  • T to C, chromosome 2 at 156,333,653 bp
  • A to T, chromosome 2 at 158,008,395 bp
  • G to A, chromosome 2 at 173,210,696 bp
  • T to C, chromosome 3 at 50,417,963 bp
  • A to G, chromosome 3 at 56,890,213 bp
  • T to C, chromosome 5 at 145,211,174 bp
  • T to C, chromosome 6 at 132,737,532 bp
  • C to T, chromosome 7 at 18,350,862 bp
  • T to C, chromosome 7 at 46,005,239 bp
  • T to C, chromosome 7 at 66,419,169 bp
  • C to T, chromosome 7 at 67,531,614 bp
  • T to C, chromosome 7 at 80,893,221 bp
  • TCC to TC, chromosome 7 at 85,221,550 bp
  • T to C, chromosome 7 at 97,484,971 bp
  • A to G, chromosome 7 at 122,220,918 bp
  • G to T, chromosome 8 at 27,025,099 bp
  • C to A, chromosome 8 at 69,302,503 bp
  • G to A, chromosome 9 at 15,998,271 bp
  • A to G, chromosome 10 at 90,049,892 bp
  • C to G, chromosome 11 at 62,780,204 bp
  • G to A, chromosome 11 at 67,856,407 bp
  • T to C, chromosome 12 at 70,984,781 bp
  • G to T, chromosome 12 at 114,746,663 bp
  • T to C, chromosome 14 at 4,398,584 bp
  • C to T, chromosome 15 at 94,403,445 bp
  • G to T, chromosome 16 at 20,678,843 bp
  • A to T, chromosome 16 at 21,545,236 bp
  • G to A, chromosome 16 at 59,344,867 bp
  • G to A, chromosome 19 at 3,935,236 bp
  • A to G, chromosome 19 at 11,896,558 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4479 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041736-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.