Strain Name:
C57BL/6J-MtgxR4482Btlr/Mmmh
Stock Number:
041738-MU
Citation ID:
RRID:MMRRC_041738-MU
Other Names:
R4482 (G1), C57BL/6J-MtgxR4482Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223838
Homologene: 11808
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: D12Ertd7e, 1110006F08Rik, Dicer1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 192119
VEGA: 12
Homologene: 13251
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Tara, Mus EST 478828, EST478828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110253
Homologene: 5104
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 3010027A04Rik, Yod, 9530048I21Rik, 2410104C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 77087
Homologene: 69134
Parg
Name: poly (ADP-ribose) glycohydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 26430
HGNC: HGNC:8605
Homologene: 50532
Git1
Name: GIT ArfGAP 1
Synonyms: p95Cat, Cat-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216963
HGNC: HGNC:4272
Homologene: 32204
Fam160a2
Name: family with sequence similarity 160, member A2
Synonyms: 6530415H11Rik, 4632419K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74349
Homologene: 75329
Cct5
Name: chaperonin containing Tcp1, subunit 5 (epsilon)
Synonyms: Ccte, TCPE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 12465
HGNC: HGNC:1618
Homologene: 6287
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 26558
Homologene: 37972
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 329002
Homologene: 7198
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: 2210407G14Rik, Hapip, E530005C20Rik, LOC224126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Plxna2
Name: plexin A2
Synonyms: 2810428A13Rik, PlexA2, Plxn2, OCT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Vasn
Name: vasorin
Synonyms: 2610528G05Rik, Slitl2, ATIA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 246154
Homologene: 36929
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Slc4a8
Name: solute carrier family 4 (anion exchanger), member 8
Synonyms: sodium bicarbonate cotransporter isoform 3 kNBC-3, KNBC-3, NDCBE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 59033
Homologene: 68419
Cyp4a10
Name: cytochrome P450, family 4, subfamily a, polypeptide 10
Synonyms: D4Rp1, RP1, Msp-3, Cyp4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13117
HGNC: HGNC:2642
Homologene: 128044
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: Ask2, MEKK6, MAPKKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
L3hypdh
Name: L-3-hydroxyproline dehydratase (trans-)
Synonyms: 2810055F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 67217
Homologene: 12100
Ctsf
Name: cathepsin F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 56464
VEGA: 19
HGNC: HGNC:2531
Homologene: 31194
Olfr190
Name: olfactory receptor 190
Synonyms: MOR183-4, GA_x54KRFPKG5P-55303207-55302284
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 258392
Homologene: 131129
Kbtbd3
Name: kelch repeat and BTB (POZ) domain containing 3
Synonyms: 2200003A07Rik, Bklhd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 69149
Homologene: 12300
4921536K21Rik
Name: RIKEN cDNA 4921536K21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67430
Homologene: 12129
Nexn
Name: nexilin
Synonyms: 1110046H09Rik, nF actin binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68810
Homologene: 44892
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74691
Homologene: 14311
Rasl10a
Name: RAS-like, family 10, member A
Synonyms: 2210403B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 75668
Homologene: 4732
Trim21
Name: tripartite motif-containing 21
Synonyms: Ro52, Ssa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20821
Homologene: 2365
Gm732
Name: predicted gene 732
Synonyms: LOC213450
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 213450
Homologene: 53031
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 194,749,317 bp
  • T to A, chromosome 2 at 71,855,915 bp
  • A to T, chromosome 2 at 125,363,610 bp
  • T to C, chromosome 3 at 152,242,753 bp
  • T to C, chromosome 4 at 115,532,598 bp
  • T to C, chromosome 4 at 133,243,399 bp
  • G to A, chromosome 7 at 102,563,933 bp
  • A to T, chromosome 7 at 105,389,674 bp
  • T to C, chromosome 8 at 25,785,044 bp
  • T to C, chromosome 8 at 40,554,384 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to C, chromosome 8 at 122,893,489 bp
  • A to T, chromosome 9 at 4,331,051 bp
  • C to A, chromosome 9 at 108,114,664 bp
  • A to G, chromosome 11 at 3,895,008 bp
  • T to A, chromosome 11 at 5,058,429 bp
  • T to A, chromosome 11 at 77,500,507 bp
  • A to G, chromosome 12 at 72,084,992 bp
  • A to G, chromosome 12 at 104,706,277 bp
  • T to C, chromosome 12 at 112,014,501 bp
  • A to G, chromosome 14 at 32,262,774 bp
  • T to C, chromosome 15 at 31,597,569 bp
  • T to C, chromosome 15 at 78,966,563 bp
  • T to C, chromosome 15 at 94,345,920 bp
  • C to A, chromosome 15 at 100,810,599 bp
  • A to T, chromosome 16 at 4,648,326 bp
  • A to G, chromosome 16 at 32,756,701 bp
  • C to T, chromosome 16 at 33,989,810 bp
  • G to T, chromosome 16 at 59,074,923 bp
  • A to T, chromosome 18 at 82,644,221 bp
  • A to T, chromosome 19 at 4,863,408 bp
  • T to A, chromosome X at 107,946,631 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4482 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041738-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.