Strain Name:
C57BL/6J-MtgxR4483Btlr/Mmmh
Stock Number:
041739-MU
Citation ID:
RRID:MMRRC_041739-MU
Other Names:
R4483 (G1), C57BL/6J-MtgxR4483Btlr
Major Collection:

Strain Information

Rnf145
Name: ring finger protein 145
Synonyms: 3732413I11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74315
Homologene: 14427
Usp9x
Name: ubiquitin specific peptidase 9, X chromosome
Synonyms: Dffrx, 5730589N07Rik, Fafl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22284
Homologene: 3418
Dbi
Name: diazepam binding inhibitor
Synonyms: diazepam-binding inhibitor, EP, Acbp, endozepine
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13167
HGNC: HGNC:2690
Homologene: 39086
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328977
Homologene: 138627
Med15
Name: mediator complex subunit 15
Synonyms: A230074L19Rik, Pcqap
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94112
VEGA: 16
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74081
Homologene: 8879
Ppp2r1a
Name: protein phosphatase 2, regulatory subunit A, alpha
Synonyms: PP2A, protein phosphatase PP2A, PR65, 6330556D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 51792
HGNC: HGNC:9302
Homologene: 68559
Zfp507
Name: zinc finger protein 507
Synonyms: 1810022O10Rik, A230056M16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 668501
Homologene: 8942
Parp11
Name: poly (ADP-ribose) polymerase family, member 11
Synonyms: 5330431N24Rik, HIN1L
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101187
HGNC: HGNC:1186
Homologene: 10680
Akap11
Name: A kinase anchor protein 11
Synonyms: 6330501D17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219181
HGNC: HGNC:369
Homologene: 8279
Fance
Name: Fanconi anemia, complementation group E
Synonyms: 2810451D06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72775
HGNC: HGNC:3586
Homologene: 11066
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mgr1, Mass1, Gpr98, VLGR1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Ctsq
Name: cathepsin Q
Synonyms: 1600010J02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 104002
Homologene: 122226
Tnfaip3
Name: tumor necrosis factor, alpha-induced protein 3
Synonyms: zinc finger protein A20, Tnfip3, A20
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21929
Homologene: 4582
Ankrd50
Name: ankyrin repeat domain 50
Synonyms: E430012K20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99696
Homologene: 129859
Txlnb
Name: taxilin beta
Synonyms: 2310001N14Rik, Mdp77
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 378431
Homologene: 44534
Pla2g4b
Name: phospholipase A2, group IVB (cytosolic)
Synonyms: A030011C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211429
Homologene: 3730
Ccdc158
Name: coiled-coil domain containing 158
Synonyms: 4932413O14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320696
Homologene: 18560
Fbxl6
Name: F-box and leucine-rich repeat protein 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 30840
VEGA: 15
Homologene: 8130
Fkbpl
Name: FK506 binding protein-like
Synonyms: WAF-1/CIP1 stabilizing protein 39, DIR1, NG7, WISp39, Ppiase-X
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56299
Homologene: 10529
Defa35
Name: defensin, alpha, 35
Synonyms: ENSMUSG00000061845, OTTMUSG00000018258, Gm10104
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100041688
Homologene: 113382
Vmn1r30
Name: vomeronasal 1 receptor 30
Synonyms: V1rc9, V1rc22
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171195
Homologene: 137652
Nlrp6
Name: NLR family, pyrin domain containing 6
Synonyms: Nalp6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101613
Homologene: 15881
Prl7a2
Name: prolactin family 7, subfamily a, member 2
Synonyms: PLP-F, Prlpf
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19114
Homologene: 49263
Cpa5
Name: carboxypeptidase A5
Synonyms: 4930430M09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74649
Homologene: 62246
Chil4
Name: chitinase-like 4
Synonyms: Chi3l4, Ym2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 104183
Homologene: 74931
Rpl31-ps17
Name: ribosomal protein L31, pseudogene 17
Synonyms: Gm16382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 673582
Gm12502
Name: predicted gene 12502
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 102640327
1700094J05Rik
Name: RIKEN cDNA 1700094J05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67523
AW112010
Name: expressed sequence AW112010
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107350
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 120,120,805 bp
  • A to G, chromosome 1 at 155,926,468 bp
  • T to C, chromosome 2 at 120,035,840 bp
  • A to G, chromosome 3 at 38,457,531 bp
  • G to A, chromosome 3 at 106,214,362 bp
  • C to T, chromosome 3 at 108,016,518 bp
  • T to A, chromosome 4 at 143,895,840 bp
  • A to G, chromosome 5 at 92,633,328 bp
  • A to G, chromosome 6 at 30,624,626 bp
  • A to T, chromosome 6 at 58,435,133 bp
  • A to G, chromosome 6 at 127,471,605 bp
  • A to G, chromosome 7 at 35,787,716 bp
  • A to G, chromosome 7 at 140,921,781 bp
  • T to C, chromosome 8 at 21,065,192 bp
  • T to C, chromosome 9 at 118,514,186 bp
  • T to TTA, chromosome 10 at 17,838,997 bp
  • A to T, chromosome 10 at 19,011,627 bp
  • A to G, chromosome 10 at 76,401,483 bp
  • T to C, chromosome 11 at 44,564,277 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • T to A, chromosome 13 at 27,660,947 bp
  • T to C, chromosome 13 at 61,038,912 bp
  • C to T, chromosome 13 at 81,419,230 bp
  • A to G, chromosome 14 at 78,510,259 bp
  • A to G, chromosome 15 at 76,537,929 bp
  • A to T, chromosome 16 at 17,671,564 bp
  • C to T, chromosome 17 at 20,955,810 bp
  • T to A, chromosome 17 at 28,315,807 bp
  • T to C, chromosome 17 at 34,646,295 bp
  • T to A, chromosome 18 at 12,549,253 bp
  • G to T, chromosome 18 at 65,656,565 bp
  • A to G, chromosome 19 at 11,050,393 bp
  • A to G, chromosome X at 13,121,448 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4483 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041739-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.