Strain Name:
C57BL/6J-MtgxR4485Btlr/Mmmh
Stock Number:
041741-MU
Citation ID:
RRID:MMRRC_041741-MU
Other Names:
R4485 (G1), C57BL/6J-MtgxR4485Btlr
Major Collection:

Gene Information

Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214444
Homologene: 49533
Hdac4
Name: histone deacetylase 4
Synonyms: 4932408F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208727
Homologene: 55946
Wdr7
Name: WD repeat domain 7
Synonyms: TGF-beta resistance associated gene, TRAG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Zfp472
Name: zinc finger protein 472
Synonyms: Krim-1B, Krim-1A, Krim-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224691
Homologene: 77422
Eps15l1
Name: epidermal growth factor receptor pathway substrate 15-like 1
Synonyms: Eps15R, 9830147J04Rik, Eps15-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13859
Homologene: 31881
Zfp219
Name: zinc finger protein 219
Synonyms: 2010302A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69890
VEGA: 14
Homologene: 9504
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Eps15
Name: epidermal growth factor receptor pathway substrate 15
Synonyms: 2410112D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13858
HGNC: HGNC:3419
Homologene: 128359
Slc16a1
Name: solute carrier family 16 (monocarboxylic acid transporters), member 1
Synonyms: MCT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20501
Homologene: 20662
Snrnp48
Name: small nuclear ribonucleoprotein 48 (U11/U12)
Synonyms: 1110050F08Rik, 6530403A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67797
VEGA: 13
Homologene: 12191
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: calcium release channel isoform 1, Ryr, skrr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20190
Homologene: 68069
Tecta
Name: tectorin alpha
Synonyms: [a]-tectorin, Tctna
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21683
Homologene: 3955
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100041194
Homologene: 131081
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: Cchl1a3, fmd, mdg, sj, muscle dysgenesis, Cav1.1, DHPR alpha1s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Gm8251
Name: predicted gene 8251
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
Capn8
Name: calpain 8
Synonyms: nCL-2', nCL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 170725
HGNC: HGNC:1485
Homologene: 15643
Olfr679
Name: olfactory receptor 679
Synonyms: GA_x6K02T2PBJ9-7714499-7715446, MOR40-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259046
Homologene: 81538
Olfr1461
Name: olfactory receptor 1461
Synonyms: GA_x6K02T2RE5P-3491834-3492772, MOR202-35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258299
Homologene: 128112
Cpb1
Name: carboxypeptidase B1 (tissue)
Synonyms: 2210008M23Rik, 0910001A18Rik, 1810063F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76703
HGNC: HGNC:2299
Homologene: 68127
Olfr1474
Name: olfactory receptor 1474
Synonyms: GA_x6K02T2RE5P-3803583-3804527, MOR202-26P, MOR202-42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258123
Homologene: 79417
Agxt2
Name: alanine-glyoxylate aminotransferase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268782
Homologene: 12887
Itprid1
Name: ITPR interacting domain containing 1
Synonyms: D530004J12Rik, Ccdc129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232016
Homologene: 52344
Gm14226
Name: predicted gene 14226
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Vmn2r87
Name: vomeronasal 2, receptor 87
Synonyms: EG625131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 625131
Homologene: 129606
Olfr482
Name: olfactory receptor 482
Synonyms: GA_x6K02T2PBJ9-10424354-10423383, MOR204-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258728
Homologene: 133604
Gbp9
Name: guanylate-binding protein 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 236573
Homologene: 128731
Npm2
Name: nucleophosmin/nucleoplasmin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328440
VEGA: 14
HGNC: HGNC:7930
Homologene: 15349
Apol7b
Name: apolipoprotein L 7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 278679
Homologene: 40940
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78895
Homologene: 12842
Psg25
Name: pregnancy-specific glycoprotein 25
Synonyms: cea13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114868
Homologene: 110989
Plat
Name: plasminogen activator, tissue
Synonyms: t-PA, tPA, D8Ertd2e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18791
HGNC: HGNC:9051
Homologene: 717
1700021A07Rik
Name: RIKEN cDNA 1700021A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Prss30
Name: protease, serine 30
Synonyms: 2010015P21Rik, Disp, Disp1, Tmprss8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 30943
Homologene: 88826
Banf2
Name: BANF family member 2
Synonyms: LOC228710, 4930517K23Rik, barrier to autointegration factor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 403171
Homologene: 18671
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,060,123 bp
  • A to T, chromosome 1 at 91,933,315 bp
  • A to G, chromosome 1 at 136,076,852 bp
  • T to C, chromosome 1 at 182,598,741 bp
  • C to T, chromosome 2 at 144,073,852 bp
  • G to A, chromosome 2 at 155,025,271 bp
  • T to G, chromosome 3 at 20,249,701 bp
  • A to G, chromosome 3 at 104,655,478 bp
  • A to T, chromosome 4 at 70,239,283 bp
  • G to A, chromosome 4 at 109,366,530 bp
  • C to G, chromosome 5 at 105,083,808 bp
  • A to G, chromosome 6 at 55,887,066 bp
  • C to A, chromosome 7 at 18,526,278 bp
  • C to T, chromosome 7 at 29,090,156 bp
  • T to A, chromosome 7 at 105,086,601 bp
  • T to C, chromosome 7 at 108,095,015 bp
  • G to A, chromosome 8 at 22,772,212 bp
  • A to T, chromosome 8 at 72,399,687 bp
  • T to A, chromosome 9 at 42,337,274 bp
  • A to G, chromosome 10 at 21,425,634 bp
  • A to T, chromosome 10 at 130,479,809 bp
  • A to G, chromosome 12 at 112,779,767 bp
  • T to A, chromosome 13 at 38,216,328 bp
  • T to C, chromosome 13 at 55,245,621 bp
  • C to T, chromosome 13 at 63,534,329 bp
  • A to G, chromosome 14 at 52,007,384 bp
  • A to T, chromosome 14 at 70,648,309 bp
  • A to T, chromosome 15 at 10,378,882 bp
  • C to A, chromosome 15 at 77,423,666 bp
  • G to T, chromosome 15 at 94,523,490 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to C, chromosome 17 at 23,973,156 bp
  • T to C, chromosome 17 at 32,977,568 bp
  • A to G, chromosome 18 at 63,777,550 bp
  • T to C, chromosome 19 at 13,165,491 bp
  • T to C, chromosome 19 at 13,471,555 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4485 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041741-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.