Strain Name:
C57BL/6J-MtgxR4487Btlr/Mmmh
Stock Number:
041743-MU
Citation ID:
RRID:MMRRC_041743-MU
Other Names:
R4487 (G1), C57BL/6J-MtgxR4487Btlr
Major Collection:

Gene Information

Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: 2610028O16Rik, D11Ertd530e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52615
Homologene: 32256
Ngf
Name: nerve growth factor
Synonyms: Ngfb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18049
HGNC: HGNC:7808
Homologene: 1876
Snx1
Name: sorting nexin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56440
Homologene: 99716
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5HT1B receptor, 5-HT1B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Smchd1
Name: SMC hinge domain containing 1
Synonyms: 4931400A14Rik, MommeD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74355
Homologene: 23665
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, 8030453L17Rik, E430018P19Rik, KMT2H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192195
Homologene: 10225
C1ql2
Name: complement component 1, q subcomponent-like 2
Synonyms: CTRP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226359
Homologene: 18792
Ablim2
Name: actin-binding LIM protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231148
Homologene: 82366
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: C730009O10Rik, 9430025M21Rik, lamellipodin, Lpd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77300
Homologene: 71030
Nepro
Name: nucleolus and neural progenitor protein
Synonyms: BC027231
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212547
Homologene: 9127
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, Hapip, 2210407G14Rik, E530005C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Tor1aip1
Name: torsin A interacting protein 1
Synonyms: LAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208263
Homologene: 9208
Pgap1
Name: post-GPI attachment to proteins 1
Synonyms: 5033403E17Rik, 9030223K07Rik, PGAP1, D230012E17Rik, oto
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241062
Homologene: 41605
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Tro
Name: trophinin
Synonyms: necdin and trophinin like, Tnn, trophinin-2, magphinin-gamma, magphinin-beta 2, magphinin-alpha, magphinin, Maged3l, Maged3, Trol
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56191
Homologene: 75113
Hcn3
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 3
Synonyms: Hac3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15168
Homologene: 22453
Kif9
Name: kinesin family member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16578
Homologene: 65620
Mapk4
Name: mitogen-activated protein kinase 4
Synonyms: A330097D03Rik, Erk3-related, p63Mapk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225724
HGNC: HGNC:6878
Homologene: 2058
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230806
Homologene: 19232
Krt76
Name: keratin 76
Synonyms: 2310001L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77055
Homologene: 22931
Oaz3
Name: ornithine decarboxylase antizyme 3
Synonyms: ornithine decarboxylase antizyme in testis, Oaz-t, AZ-3, antizyme 3, AZ3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53814
HGNC: HGNC:8097
Homologene: 9410
Vmn1r61
Name: vomeronasal 1 receptor 61
Synonyms: Gm7186
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 636731
Homologene: 41799
Rusf1
Name: RUS family member 1
Synonyms: BC017158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233913
Homologene: 11232
Nt5m
Name: 5',3'-nucleotidase, mitochondrial
Synonyms: 2010013E09Rik, dNT-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103850
Homologene: 10622
Rftn2
Name: raftlin family member 2
Synonyms: 2700010E02Rik, 3222401M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74013
Homologene: 16954
Abcd2
Name: ATP-binding cassette, sub-family D (ALD), member 2
Synonyms: ALDR, ALDL1, adrenoleukodystrophy related, ABC39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26874
VEGA: 15
HGNC: HGNC:66
Homologene: 55873
Hao2
Name: hydroxyacid oxidase 2
Synonyms: Hao3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56185
HGNC: HGNC:4810
Homologene: 97412
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78895
Homologene: 12842
Mup4
Name: major urinary protein 4
Synonyms: Mup-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17843
Homologene: 74304
2310030G06Rik
Name: RIKEN cDNA 2310030G06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66952
VEGA: 9
Homologene: 12059
Rnase2a
Name: ribonuclease, RNase A family, 2A (liver, eosinophil-derived neurotoxin)
Synonyms: Ear11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93726
VEGA: 14
Homologene: 121614
Cnga2
Name: cyclic nucleotide gated channel alpha 2
Synonyms: OCNC1, Cnca, Cncg4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 12789
HGNC: HGNC:2149
Homologene: 32022
4930432L08Rik
Name: RIKEN cDNA 4930432L08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74621
Gm26694
Name: predicted gene, 26694
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Xlr5a
Name: X-linked lymphocyte-regulated 5A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 574438
Homologene: 41832
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 54,528,592 bp
  • A to G, chromosome 1 at 55,202,152 bp
  • C to T, chromosome 1 at 60,502,869 bp
  • A to T, chromosome 1 at 120,341,680 bp
  • G to T, chromosome 1 at 156,007,124 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • T to A, chromosome 3 at 88,985,315 bp
  • A to G, chromosome 3 at 89,148,826 bp
  • A to T, chromosome 3 at 94,435,130 bp
  • T to A, chromosome 3 at 98,882,025 bp
  • G to A, chromosome 3 at 102,520,699 bp
  • T to A, chromosome 4 at 59,960,547 bp
  • T to C, chromosome 4 at 134,074,201 bp
  • A to G, chromosome 4 at 148,051,427 bp
  • C to A, chromosome 5 at 35,841,346 bp
  • A to G, chromosome 5 at 76,344,062 bp
  • C to A, chromosome 7 at 5,610,925 bp
  • T to C, chromosome 7 at 128,288,358 bp
  • A to G, chromosome 9 at 50,740,631 bp
  • A to T, chromosome 9 at 66,089,595 bp
  • A to T, chromosome 9 at 81,631,539 bp
  • A to G, chromosome 9 at 110,494,484 bp
  • A to G, chromosome 11 at 59,848,347 bp
  • A to G, chromosome 11 at 80,032,113 bp
  • A to G, chromosome 13 at 81,440,066 bp
  • A to T, chromosome 14 at 51,255,845 bp
  • G to A, chromosome 15 at 66,671,396 bp
  • A to G, chromosome 15 at 91,178,283 bp
  • A to G, chromosome 15 at 94,531,617 bp
  • T to C, chromosome 15 at 101,890,482 bp
  • C to T, chromosome 16 at 33,989,810 bp
  • A to G, chromosome 16 at 44,735,726 bp
  • G to A, chromosome 17 at 25,839,493 bp
  • T to C, chromosome 17 at 71,407,235 bp
  • T to C, chromosome 18 at 73,930,975 bp
  • T to A, chromosome X at 72,006,127 bp
  • T to C, chromosome X at 73,157,898 bp
  • T to C, chromosome X at 150,653,325 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4487 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041743-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.