Strain Name:
C57BL/6J-MtgxR4501Btlr/Mmmh
Stock Number:
041753-MU
Citation ID:
RRID:MMRRC_041753-MU
Other Names:
R4501 (G1), C57BL/6J-MtgxR4501Btlr
Major Collection:

Gene Information

Rpl13a
Name: ribosomal protein L13A
Synonyms: 1810026N22Rik, tum-antigen, Tstap198-7, tum-transplantation antigen P198
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22121
Homologene: 111053
Ptpn12
Name: protein tyrosine phosphatase, non-receptor type 12
Synonyms: PTP-PEST, P19-PTP, PTP-P19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19248
HGNC: HGNC:9645
Homologene: 37691
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22225
Homologene: 55758
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 277854
Homologene: 34718
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: D3Ertd775e, Lba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Usp34
Name: ubiquitin specific peptidase 34
Synonyms: Murr2, A530081C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17847
Homologene: 40978
Acin1
Name: apoptotic chromatin condensation inducer 1
Synonyms: Acinus, 2610036I19Rik, 2610510L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 56215
Homologene: 22853
Ankrd28
Name: ankyrin repeat domain 28
Synonyms: E430019N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105522
VEGA: 14
Homologene: 35374
Taf1c
Name: TATA-box binding protein associated factor, RNA polymerase I, C
Synonyms: mTAFI95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 21341
Homologene: 21163
Nxn
Name: nucleoredoxin
Synonyms: l11Jus13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18230
Homologene: 69028
Tnrc6c
Name: trinucleotide repeat containing 6C
Synonyms: 9930033H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217351
Homologene: 23137
Zbtb44
Name: zinc finger and BTB domain containing 44
Synonyms: Btbd15, 6030404E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235132
VEGA: 9
Homologene: 18221
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 545370
Homologene: 23741
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: 2900022L05Rik, Dnahc2, D330014H01Rik, Dnhd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Dennd4a
Name: DENN/MADD domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102442
VEGA: 9
Homologene: 55933
Hc
Name: hemolytic complement
Synonyms: C5, C5a, Hfib2, He
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 15139
HGNC: HGNC:1331
Homologene: 20412
Phldb3
Name: pleckstrin homology like domain, family B, member 3
Synonyms: EG232970, Gm10102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232970
Homologene: 109375
Atp2a1
Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Synonyms: SERCA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11937
HGNC: HGNC:811
Homologene: 7635
Slc30a4
Name: solute carrier family 30 (zinc transporter), member 4
Synonyms: Znt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22785
Homologene: 74984
AU018091
Name: expressed sequence AU018091
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 245128
Homologene: 106376
Cdh2
Name: cadherin 2
Synonyms: Ncad, N-CAD, N-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240776
Homologene: 16121
Evi5l
Name: ecotropic viral integration site 5 like
Synonyms: 2310039H16Rik, 3110007G05Rik, B130050I23Rik, 1700084G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 213027
Homologene: 134635
Vmn1r80
Name: vomeronasal 1 receptor 80
Synonyms: V1rg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 171238
Pidd1
Name: p53 induced death domain protein 1
Synonyms: Pidd, Lrdd, 1200011D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 57913
Homologene: 11220
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74691
Homologene: 14311
Ncf2
Name: neutrophil cytosolic factor 2
Synonyms: NADPH oxidase subunit (67kDa), Ncf-2, p67phox, NOXA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17970
HGNC: HGNC:7661
Homologene: 374
Plk5
Name: polo like kinase 5
Synonyms: 6330514A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216166
Homologene: 28072
Mmd2
Name: monocyte to macrophage differentiation-associated 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 75104
Homologene: 18425
Pldi
Name: polymorphic derived intron containing
Synonyms: 1700125F08Rik, Poldi
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73616
P2ry12
Name: purinergic receptor P2Y, G-protein coupled 12
Synonyms: P2Y12, 2900079B22Rik, 4921504D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 70839
Homologene: 11260
Sh3d21
Name: SH3 domain containing 21
Synonyms: 1700029G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66938
Homologene: 12057
Dusp6
Name: dual specificity phosphatase 6
Synonyms: MKP-3, MKP3, PYST1, 1300019I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67603
VEGA: 10
HGNC: HGNC:3072
Homologene: 55621
Pusl1
Name: pseudouridylate synthase-like 1
Synonyms: 2810021I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433813
Homologene: 13655
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 140,552,980 bp
  • A to T, chromosome 1 at 150,633,666 bp
  • C to G, chromosome 1 at 152,835,033 bp
  • A to T, chromosome 2 at 34,997,476 bp
  • T to A, chromosome 2 at 76,794,647 bp
  • A to G, chromosome 2 at 122,685,216 bp
  • A to G, chromosome 3 at 59,217,657 bp
  • T to C, chromosome 3 at 86,542,582 bp
  • GAATCTCCTGGGAAAATC to GAATC, chromosome 4 at 126,162,859 bp
  • T to C, chromosome 4 at 155,889,542 bp
  • G to T, chromosome 5 at 21,019,280 bp
  • T to G, chromosome 5 at 32,944,028 bp
  • A to G, chromosome 5 at 142,575,210 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • A to G, chromosome 7 at 3,159,079 bp
  • A to G, chromosome 7 at 12,193,391 bp
  • A to G, chromosome 7 at 24,612,561 bp
  • T to C, chromosome 7 at 45,126,140 bp
  • G to A, chromosome 7 at 126,453,383 bp
  • A to G, chromosome 7 at 141,441,443 bp
  • T to C, chromosome 8 at 4,205,351 bp
  • A to G, chromosome 8 at 119,599,429 bp
  • G to A, chromosome 9 at 31,054,166 bp
  • G to A, chromosome 9 at 64,910,123 bp
  • G to T, chromosome 10 at 60,928,409 bp
  • T to C, chromosome 10 at 80,359,471 bp
  • T to A, chromosome 10 at 99,264,595 bp
  • C to A, chromosome 11 at 23,401,529 bp
  • T to A, chromosome 11 at 69,477,659 bp
  • T to C, chromosome 11 at 76,274,612 bp
  • T to A, chromosome 11 at 117,722,498 bp
  • A to G, chromosome 12 at 112,042,809 bp
  • A to G, chromosome 14 at 31,706,796 bp
  • A to G, chromosome 14 at 54,686,587 bp
  • A to G, chromosome 18 at 16,629,585 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4501 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041753-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.