Strain Name:
C57BL/6J-MtgxR4504Btlr/Mmmh
Stock Number:
041755-MU
Citation ID:
RRID:MMRRC_041755-MU
Other Names:
R4504 (G1), C57BL/6J-MtgxR4504Btlr
Major Collection:

Gene Information

Lhx5
Name: LIM homeobox protein 5
Synonyms: Lim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16873
Homologene: 40621
Pdgfc
Name: platelet-derived growth factor, C polypeptide
Synonyms: 1110064L01Rik, PDGF-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 54635
HGNC: HGNC:8801
Homologene: 9423
Jarid2
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Ppp4c
Name: protein phosphatase 4, catalytic subunit
Synonyms: 1110002D08Rik, PPX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56420
HGNC: HGNC:9319
Homologene: 2038
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216161
VEGA: 10
Homologene: 8981
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Pole2
Name: polymerase (DNA directed), epsilon 2 (p59 subunit)
Synonyms: DNA polymerase epsilon small subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 18974
VEGA: 12
HGNC: HGNC:9178
Homologene: 2015
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: Grit, 3426406O18Rik, PX-RICS, GC-GAP, p200RhoGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 330914
Homologene: 8812
Golga1
Name: golgi autoantigen, golgin subfamily a, 1
Synonyms: awag, golgin-97, 0710001G09Rik, 2210418B03Rik, Golgi97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76899
HGNC: HGNC:4424
Homologene: 68223
Dsg4
Name: desmoglein 4
Synonyms: CDHF13, lah
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16769
Homologene: 65341
Notch1
Name: notch 1
Synonyms: lin-12, Tan1, N1, Motch A, 9930111A19Rik, Mis6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18128
HGNC: HGNC:7881
Homologene: 32049
Kif3b
Name: kinesin family member 3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16569
HGNC: HGNC:6320
Homologene: 55849
Carm1
Name: coactivator-associated arginine methyltransferase 1
Synonyms: Prmt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 59035
Homologene: 10990
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930543C13Rik, 4930516E05Rik, D330038K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Pcsk5
Name: proprotein convertase subtilisin/kexin type 5
Synonyms: PC5A, PC6, PC5/6A, b2b585Clo, b2b1549Clo, SPC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18552
VEGA: 19
HGNC: HGNC:8747
Homologene: 21244
Pdzd8
Name: PDZ domain containing 8
Synonyms: Pdzk8, A630041P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107368
VEGA: 19
Homologene: 14879
Gm13023
Name: predicted gene 13023
Synonyms: MGC:91194
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 194227
Homologene: 103830
Olfr1258
Name: olfactory receptor 1258
Synonyms: MOR232-3, GA_x6K02T2Q125-51361752-51362687
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258980
Homologene: 82299
Itih1
Name: inter-alpha trypsin inhibitor, heavy chain 1
Synonyms: inter-alpha (globulin) inhibitor, H1 polypeptide, Itih-1, Intin1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 16424
HGNC: HGNC:6166
Homologene: 1667
Pkn1
Name: protein kinase N1
Synonyms: Pkn, F730027O18Rik, PAK1, Stk3, Prkcl1, PRK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 320795
HGNC: HGNC:9405
Homologene: 48130
Kcnc2
Name: potassium voltage gated channel, Shaw-related subfamily, member 2
Synonyms: KShIIIA, Kv3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 268345
VEGA: 10
HGNC: HGNC:6234
Homologene: 71199
Dstyk
Name: dual serine/threonine and tyrosine protein kinase
Synonyms: A930019K20Rik, C430014H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 213452
Homologene: 19711
Npc1l1
Name: NPC1 like intracellular cholesterol transporter 1
Synonyms: Niemann-Pick disease, type C1, 9130221N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237636
HGNC: HGNC:7898
Homologene: 56585
Rad51d
Name: RAD51 paralog D
Synonyms: Rad51l3, R51H3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 19364
HGNC: HGNC:9823
Homologene: 2156
Alox5
Name: arachidonate 5-lipoxygenase
Synonyms: 5-LOX, 5LO, 5LX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11689
HGNC: HGNC:435
Homologene: 561
Scnn1b
Name: sodium channel, nonvoltage-gated 1 beta
Synonyms: ENaC beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20277
Homologene: 133555
Ric8a
Name: RIC8 guanine nucleotide exchange factor A
Synonyms: synembryn, RIC-8, Ric8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101489
Homologene: 23331
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: Krc, E030045D18Rik, Schnurri-3, 2900056N03Rik, Shn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16656
Homologene: 7803
Kcnq3
Name: potassium voltage-gated channel, subfamily Q, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110862
HGNC: HGNC:6297
Homologene: 20949
Fbh1
Name: F-box DNA helicase 1
Synonyms: Fbxo18, Fbx18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 50755
Homologene: 9272
Rnf144a
Name: ring finger protein 144A
Synonyms: Rnf144, UIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 108089
VEGA: 12
Homologene: 40982
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229534
Homologene: 10740
Krtcap2
Name: keratinocyte associated protein 2
Synonyms: 0610010I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 66059
Homologene: 15494
Taar3
Name: trace amine-associated receptor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 493809
HGNC: HGNC:4513
Homologene: 117490
Olfr279
Name: olfactory receptor 279
Synonyms: GA_x6K02T2NBG7-5241885-5241019, Olfr234, MOR122-1, GA_x6K02T04SN1-554-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 258502
Homologene: 74008
Epha10
Name: Eph receptor A10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230735
Homologene: 67804
Ear1
Name: eosinophil-associated, ribonuclease A family, member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 13586
Homologene: 40596
Anxa13
Name: annexin A13
Synonyms: 1810034H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 69787
VEGA: 15
HGNC: HGNC:536
Homologene: 20908
Adgrg4
Name: adhesion G protein-coupled receptor G4
Synonyms: PGR17, LOC236798, Gpr112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 236798
Homologene: 72131
Olfr160
Name: olfactory receptor 160
Synonyms: MOR171-3, GA_x6K02T2PVTD-31389446-31388517, M72, MOR171-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 80706
HGNC: HGNC:8469
Homologene: 12726
Pcdhgc4
Name: protocadherin gamma subfamily C, 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93707
HGNC: HGNC:8717
Homologene: 110932
RP24-260D21.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 132,434,389 bp
  • A to G, chromosome 1 at 139,085,927 bp
  • A to G, chromosome 2 at 11,749,017 bp
  • A to G, chromosome 2 at 26,472,177 bp
  • T to C, chromosome 2 at 39,023,454 bp
  • C to T, chromosome 2 at 89,930,351 bp
  • T to C, chromosome 2 at 153,323,644 bp
  • A to C, chromosome 3 at 81,174,991 bp
  • A to G, chromosome 3 at 89,246,256 bp
  • A to G, chromosome 3 at 89,446,383 bp
  • G to A, chromosome 4 at 119,733,793 bp
  • A to G, chromosome 4 at 124,915,687 bp
  • G to C, chromosome 4 at 143,793,983 bp
  • C to A, chromosome 5 at 120,440,008 bp
  • G to A, chromosome 6 at 116,420,379 bp
  • A to C, chromosome 7 at 121,912,475 bp
  • A to C, chromosome 7 at 126,787,465 bp
  • A to G, chromosome 7 at 140,858,516 bp
  • G to A, chromosome 8 at 83,692,927 bp
  • T to C, chromosome 9 at 21,569,526 bp
  • C to A, chromosome 9 at 32,181,839 bp
  • C to T, chromosome 9 at 37,711,464 bp
  • A to G, chromosome 10 at 23,949,573 bp
  • C to A, chromosome 10 at 80,060,492 bp
  • T to A, chromosome 10 at 81,315,111 bp
  • T to C, chromosome 10 at 112,455,794 bp
  • A to G, chromosome 11 at 6,228,741 bp
  • T to A, chromosome 11 at 82,892,400 bp
  • T to A, chromosome 12 at 26,327,303 bp
  • A to G, chromosome 12 at 69,222,468 bp
  • A to G, chromosome 13 at 44,914,869 bp
  • G to A, chromosome 14 at 30,935,885 bp
  • A to T, chromosome 14 at 43,819,264 bp
  • T to C, chromosome 15 at 58,356,203 bp
  • A to T, chromosome 15 at 65,995,342 bp
  • T to C, chromosome 15 at 98,498,134 bp
  • A to G, chromosome 18 at 20,461,436 bp
  • G to C, chromosome 18 at 37,816,763 bp
  • T to C, chromosome 19 at 16,695,502 bp
  • C to T, chromosome 19 at 17,451,955 bp
  • T to C, chromosome 19 at 59,345,448 bp
  • C to T, chromosome X at 56,916,442 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4504 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041755-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.