Strain Name:
C57BL/6J-MtgxR4525Btlr/Mmmh
Stock Number:
041767-MU
Citation ID:
RRID:MMRRC_041767-MU
Other Names:
R4525 (G1), C57BL/6J-MtgxR4525Btlr
Major Collection:

Gene Information

Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Mga
Name: MAX gene associated
Synonyms: D030062C11Rik, C130042M01Rik, Mga, Mad5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29808
Homologene: 49351
Tnpo3
Name: transportin 3
Synonyms: 5730544L10Rik, C430013M08Rik, D6Ertd313e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320938
Homologene: 40848
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: 2010321I05Rik, CAGH26, Tnrc6, D130023A07Rik, 3110054G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233833
Homologene: 41399
Cpne3
Name: copine III
Synonyms: CPN3, 5730450C07Rik, PRO1071, 5430428M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70568
HGNC: HGNC:2316
Homologene: 20839
Erbin
Name: Erbb2 interacting protein
Synonyms: Erbin, 1700028E05Rik, Erbb2ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 59079
Homologene: 41282
Rsf1
Name: remodeling and spacing factor 1
Synonyms: Hbxap, p325, C030033M12Rik, 4832420A03Rik, XAP8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, Ankyrin-G, Ankyrin-3, 2900054D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Pon1
Name: paraoxonase 1
Synonyms: Pon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18979
HGNC: HGNC:9204
Homologene: 68058
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72745
Homologene: 14519
Slit2
Name: slit guidance ligand 2
Synonyms: b2b1200.1Clo, E030015M03Rik, E130320P19Rik, Drad-1, Slil3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20563
Homologene: 3516
Hnrnpk
Name: heterogeneous nuclear ribonucleoprotein K
Synonyms: Hnrpk, KBBP, hnRNPK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15387
HGNC: HGNC:5044
Homologene: 81909
Kcna4
Name: potassium voltage-gated channel, shaker-related subfamily, member 4
Synonyms: Kv1.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16492
HGNC: HGNC:6222
Homologene: 20514
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: erythroid, Spna-1, ihj, Spna1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20739
Homologene: 74460
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20192
Homologene: 68151
Oplah
Name: 5-oxoprolinase (ATP-hydrolysing)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75475
HGNC: HGNC:8149
Homologene: 90938
Vmn2r70
Name: vomeronasal 2, receptor 70
Synonyms: EG620835
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 670940
Homologene: 115466
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: alphaA-CRYBP1, Cryabp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Shank1
Name: SH3 and multiple ankyrin repeat domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243961
Homologene: 22949
Aoc1
Name: amine oxidase, copper-containing 1
Synonyms: Abp1, 1600012D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76507
HGNC: HGNC:80
Homologene: 68159
Speer4a
Name: spermatogenesis associated glutamate (E)-rich protein 4A
Synonyms: 1700027N01Rik, SPEER-4A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75657
Homologene: 69402
Asap2
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms: Ddef2, 6530401G17Rik, LOC385250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211914
HGNC: HGNC:2721
Homologene: 2888
Ampd1
Name: adenosine monophosphate deaminase 1
Synonyms: Ampd-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229665
HGNC: HGNC:468
Homologene: 20
Tas2r140
Name: taste receptor, type 2, member 140
Synonyms: TRB3, mTRB3, Tas2r40, TRB5, mt2r64, T2R40, Tas2r13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387616
Homologene: 87013
Sipa1
Name: signal-induced proliferation associated gene 1
Synonyms: SPA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20469
VEGA: 19
Homologene: 7940
9430097D07Rik
Name: RIKEN cDNA 9430097D07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319245
VEGA: 2
Cd47
Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Synonyms: Itgp, integrin-associated protein, IAP, 9130415E20Rik, B430305P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16423
HGNC: HGNC:1682
Homologene: 1346
Iqcf4
Name: IQ motif containing F4
Synonyms: 1700042N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67320
Homologene: 122079
Rps13-ps2
Name: ribosomal protein S13, pseudogene 2
Synonyms: Gm10159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100039924
Cer1
Name: cerberus 1, DAN family BMP antagonist
Synonyms: Cerl1, Cerr1, cer-1, Cerberus-like, Cerl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12622
HGNC: HGNC:1862
Homologene: 3983
Gm17542
Name: predicted gene, 17542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 174,207,110 bp
  • A to G, chromosome 2 at 32,574,376 bp
  • C to A, chromosome 2 at 107,295,065 bp
  • A to G, chromosome 2 at 112,653,621 bp
  • A to G, chromosome 2 at 119,941,793 bp
  • T to C, chromosome 3 at 103,094,733 bp
  • G to T, chromosome 4 at 19,523,206 bp
  • A to G, chromosome 4 at 82,884,669 bp
  • A to T, chromosome 5 at 26,039,343 bp
  • G to T, chromosome 5 at 48,249,873 bp
  • T to C, chromosome 6 at 5,177,412 bp
  • T to C, chromosome 6 at 29,561,398 bp
  • A to G, chromosome 6 at 48,906,675 bp
  • T to C, chromosome 6 at 133,055,244 bp
  • A to T, chromosome 7 at 44,354,590 bp
  • A to T, chromosome 7 at 85,559,579 bp
  • C to T, chromosome 7 at 88,530,643 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,926 bp
  • A to G, chromosome 7 at 105,682,806 bp
  • A to G, chromosome 7 at 123,179,782 bp
  • T to A, chromosome 9 at 106,570,628 bp
  • T to C, chromosome 10 at 58,713,613 bp
  • T to C, chromosome 10 at 69,978,492 bp
  • G to A, chromosome 12 at 21,229,292 bp
  • T to A, chromosome 13 at 42,155,813 bp
  • A to G, chromosome 13 at 58,393,882 bp
  • T to C, chromosome 13 at 84,257,802 bp
  • T to C, chromosome 13 at 103,857,092 bp
  • G to T, chromosome 15 at 76,305,509 bp
  • T to C, chromosome 16 at 49,867,792 bp
  • T to A, chromosome 18 at 77,356,912 bp
  • T to A, chromosome 19 at 5,651,957 bp
  • A to G, chromosome 19 at 5,746,359 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4525 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041767-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.