Strain Name:
C57BL/6J-MtgxR4530Btlr/Mmmh
Stock Number:
041770-MU
Citation ID:
RRID:MMRRC_041770-MU
Other Names:
R4530 (G1), C57BL/6J-MtgxR4530Btlr
Major Collection:

Gene Information

Arhgef7
Name: Rho guanine nucleotide exchange factor (GEF7)
Synonyms: Cool, PIX, Pak interacting exchange factor, p85SPR, betaPix-c, betaPix-b, cool-1, betaPix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54126
Homologene: 2895
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Arid4b
Name: AT rich interactive domain 4B (RBP1-like)
Synonyms: RBBP1L1, SAP180, BRCAA1, 6720480E17Rik, 6330417L24Rik, Rbp1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94246
Homologene: 12847
Zfp930
Name: zinc finger protein 930
Synonyms: zinc finger protein, D10627
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234358
Axin1
Name: axin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12005
HGNC: HGNC:903
Homologene: 2614
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22129
Homologene: 2487
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Stip1
Name: stress-induced phosphoprotein 1
Synonyms: p60, IEF SSP 3521, Hop, Hsp70/Hsp90 organizing protein, STI1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20867
VEGA: 19
Homologene: 4965
Cdc27
Name: cell division cycle 27
Synonyms: APC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217232
HGNC: HGNC:1728
Homologene: 960
Arhgap42
Name: Rho GTPase activating protein 42
Synonyms: 9030420J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71544
Homologene: 28446
Lin54
Name: lin-54 DREAM MuvB core complex component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231506
Homologene: 18212
Adcy4
Name: adenylate cyclase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 104110
VEGA: 14
HGNC: HGNC:235
Homologene: 23149
Clec4e
Name: C-type lectin domain family 4, member e
Synonyms: Mincle, Clecsf9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56619
Homologene: 22809
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Il16
Name: interleukin 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16170
HGNC: HGNC:5980
Homologene: 18157
Mroh7
Name: maestro heat-like repeat family member 7
Synonyms: LOC381538, Gm1027, Heatr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381538
Homologene: 19633
Intu
Name: inturned planar cell polarity protein
Synonyms: 9430087H23Rik, 9230116I04Rik, Pdzk6, Pdzd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 380614
Homologene: 41059
Dner
Name: delta/notch-like EGF repeat containing
Synonyms: BET, A930026D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227325
Homologene: 26722
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101095
Homologene: 2647
Prss43
Name: protease, serine 43
Synonyms: LOC272643, Tessp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 272643
Homologene: 138484
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170571
Homologene: 24912
Kif21a
Name: kinesin family member 21A
Synonyms: N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16564
VEGA: 15
Homologene: 56761
Gpr158
Name: G protein-coupled receptor 158
Synonyms: 5330427M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241263
Homologene: 19381
Or8k38
Name: olfactory receptor family 8 subfamily K member 38
Synonyms: GA_x6K02T2Q125-48147264-48146323, MOR191-1, Olfr1085
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258583
Wrap73
Name: WD repeat containing, antisense to Trp73
Synonyms: DD57, 2610044M17Rik, 5330425N03Rik, Wdr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 59002
Homologene: 9857
Clec2h
Name: C-type lectin domain family 2, member h
Synonyms: Clrf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94071
Homologene: 131164
Plbd1
Name: phospholipase B domain containing 1
Synonyms: 1100001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66857
Homologene: 11745
Or2h1b
Name: olfactory receptor family 2 subfamily H member 1B
Synonyms: MOR256-39P, GA_x6K02T2PSCP-1592036-1591098, Olfr93
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258051
HGNC: HGNC:8252
Homologene: 79436
Tat
Name: tyrosine aminotransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234724
Homologene: 37293
Or10g6
Name: olfactory receptor family 10 subfamily G member 6
Synonyms: GA_x6K02T2PVTD-33720892-33721824, MOR223-8, Olfr981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258283
VEGA: 9
Homologene: 64849
Or8g2b
Name: olfactory receptor family 8 subfamily G member 2B
Synonyms: GA_x6K02T2PVTD-33539896-33540819, MOR171-13, Olfr971
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258607
VEGA: 9
Homologene: 133635
Tmprss11a
Name: transmembrane protease, serine 11a
Synonyms: LOC194597
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 194597
Homologene: 62723
Cetn4
Name: centrin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 207175
Homologene: 138302
Fam122c
Name: family with sequence similarity 122, member C
Synonyms: 4930527G05Rik, 4930432H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73866
Homologene: 87062
Xndc1
Name: Xrcc1 N-terminal domain containing 1
Synonyms: Xndr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102443350
Homologene: 128476
Gm26483
Name: predicted gene, 26483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 84,583,015 bp
  • A to G, chromosome 2 at 21,369,000 bp
  • T to C, chromosome 2 at 86,657,561 bp
  • C to T, chromosome 3 at 37,309,945 bp
  • T to G, chromosome 3 at 40,683,364 bp
  • T to C, chromosome 3 at 138,957,425 bp
  • T to C, chromosome 4 at 106,720,437 bp
  • A to G, chromosome 4 at 154,156,707 bp
  • T to A, chromosome 5 at 4,043,948 bp
  • C to A, chromosome 5 at 86,428,681 bp
  • G to A, chromosome 5 at 100,446,560 bp
  • C to T, chromosome 6 at 47,890,633 bp
  • T to C, chromosome 6 at 123,289,774 bp
  • A to G, chromosome 6 at 128,662,494 bp
  • T to A, chromosome 6 at 136,651,825 bp
  • A to C, chromosome 7 at 83,681,310 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,923 bp
  • C to A, chromosome 7 at 102,078,735 bp
  • T to C, chromosome 8 at 11,800,802 bp
  • G to T, chromosome 8 at 12,663,932 bp
  • C to T, chromosome 8 at 69,228,831 bp
  • T to A, chromosome 8 at 109,996,210 bp
  • T to C, chromosome 8 at 112,858,210 bp
  • T to C, chromosome 9 at 9,011,432 bp
  • G to T, chromosome 9 at 39,840,083 bp
  • G to A, chromosome 9 at 40,023,293 bp
  • A to T, chromosome 9 at 110,829,504 bp
  • T to C, chromosome 11 at 104,528,426 bp
  • C to A, chromosome 13 at 14,126,455 bp
  • A to T, chromosome 13 at 89,704,028 bp
  • T to C, chromosome 14 at 55,779,028 bp
  • A to C, chromosome 15 at 90,968,089 bp
  • T to A, chromosome 16 at 94,466,877 bp
  • T to A, chromosome 17 at 26,188,172 bp
  • C to T, chromosome 17 at 37,151,607 bp
  • G to T, chromosome 19 at 7,035,658 bp
  • G to A, chromosome X at 53,293,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4530 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041770-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.