Strain Name:
C57BL/6J-MtgxR4532Btlr/Mmmh
Stock Number:
041772-MU
Citation ID:
RRID:MMRRC_041772-MU
Other Names:
R4532 (G1), C57BL/6J-MtgxR4532Btlr
Major Collection:

Strain Information

Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4P, mMmORC4, Orc4, Orc4l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: rim2, recombination induced mutation 2, Hps2, beta3A, AP-3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Arhgap42
Name: Rho GTPase activating protein 42
Synonyms: 9030420J04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71544
Homologene: 28446
Lin54
Name: lin-54 DREAM MuvB core complex component
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231506
Homologene: 18212
Lpin1
Name: lipin 1
Synonyms: Lipin1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14245
VEGA: 12
Homologene: 9266
Oma1
Name: OMA1 zinc metallopeptidase
Synonyms: 2010001O09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67013
Homologene: 12070
Tes
Name: testin LIM domain protein
Synonyms: Tes2, Tes1, testin2, testin, D6Ertd352e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21753
Homologene: 41051
Heatr6
Name: HEAT repeat containing 6
Synonyms: 2700008B19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217026
Homologene: 11116
Zfp655
Name: zinc finger protein 655
Synonyms: 9030409O18Rik, 2700038I16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72611
Homologene: 12473
Pdgfra
Name: platelet derived growth factor receptor, alpha polypeptide
Synonyms: CD140a, Pdgfr-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18595
HGNC: HGNC:8803
Homologene: 31361
Cdh23
Name: cadherin related 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, nmf112, nmf181, nmf252, bob, sals
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22295
Homologene: 11142
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217463
VEGA: 12
Homologene: 41011
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64817
Homologene: 23386
Cdt1
Name: chromatin licensing and DNA replication factor 1
Synonyms: 2610318F11Rik, Ris2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67177
Homologene: 32650
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101095
Homologene: 2647
Me3
Name: malic enzyme 3, NADP(+)-dependent, mitochondrial
Synonyms: B230207H15Rik, 1700020C08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 109264
HGNC: HGNC:6985
Homologene: 100773
Rmnd5a
Name: required for meiotic nuclear division 5 homolog A
Synonyms: 1110007A06Rik, Gid2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68477
Homologene: 5668
Vmn2r75
Name: vomeronasal 2, receptor 75
Synonyms: EG546981
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546981
Homologene: 115466
Chaf1b
Name: chromatin assembly factor 1, subunit B
Synonyms: 2600017H24Rik, MPHOSPH7, CAF-I 60 kDa subunit, CAF-1 subunit B, CAF-IP60, CAF1P60, CAF1A, CAF1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110749
HGNC: HGNC:1911
Homologene: 48346
Cyp26c1
Name: cytochrome P450, family 26, subfamily c, polypeptide 1
Synonyms: EG546726
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 546726
VEGA: 19
Homologene: 28089
Slc6a15
Name: solute carrier family 6 (neurotransmitter transporter), member 15
Synonyms: v7-3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103098
VEGA: 10
Homologene: 18163
Slc39a10
Name: solute carrier family 39 (zinc transporter), member 10
Synonyms: 2900042E17Rik, Zip10
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227059
Homologene: 34076
Gys2
Name: glycogen synthase 2
Synonyms: LGS, glycogen synthase, liver
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232493
HGNC: HGNC:4707
Homologene: 56580
Aoc1l1
Name: amine oxidase copper containing 1-like 1
Synonyms: Doxl2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243376
HGNC: HGNC:80
Homologene: 19443
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: 5730420O05Rik, SALM1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70530
Homologene: 19182
Fhod3
Name: formin homology 2 domain containing 3
Synonyms: A930009H06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225288
VEGA: 18
Homologene: 45323
Stard6
Name: StAR related lipid transfer domain containing 6
Synonyms: 4833424I06Rik, 4933429L05Rik, 1700011K09Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170461
Homologene: 12578
Gm12185
Name: predicted gene 12185
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 620913
Homologene: 83188
Msln
Name: mesothelin
Synonyms: megakaryocyte potentiating factor, MPF
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56047
VEGA: 17
HGNC: HGNC:7371
Homologene: 4249
Or5al6
Name: olfactory receptor family 5 subfamily AL member 6
Synonyms: GA_x6K02T2Q125-47615732-47614791, MOR185-12, Olfr1040
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404323
Homologene: 79382
Or5p4
Name: olfactory receptor family 5 subfamily P member 4
Synonyms: GA_x6K02T2PBJ9-10409785-10410723, MOR204-2, MOR204-39, Olfr481
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258927
Homologene: 105166
Vmn1r23
Name: vomeronasal 1 receptor 23
Synonyms: V1rc24
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171197
Homologene: 110825
Vmn1r38
Name: vomeronasal 1 receptor 38
Synonyms: V1rc13
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171186
Homologene: 110800
Slco5a1
Name: solute carrier organic anion transporter family, member 5A1
Synonyms: A630033C23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240726
Homologene: 57135
Ggh
Name: gamma-glutamyl hydrolase
Synonyms: gamma-GH
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14590
HGNC: HGNC:4248
Homologene: 2881
Pabir3
Name: PABIR family member 3
Synonyms: 4930527G05Rik, 4930432H15Rik, Fam122c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73866
Homologene: 87062
Fam115c
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Lrrc3c
Name: leucine rich repeat containing 3C
Synonyms: LOC192852, Gm12
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192852
Homologene: 19077
Snora28
Name: small nucleolar RNA, H/ACA box 28
Synonyms: MBI-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100316932
VEGA: 12
Gm21095
Name: predicted gene, 21095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
Homologene: 134606
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 12,879,223 bp
  • A to G, chromosome 1 at 46,819,183 bp
  • C to A, chromosome 2 at 27,177,331 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • A to T, chromosome 2 at 86,145,930 bp
  • T to A, chromosome 4 at 20,046,225 bp
  • G to T, chromosome 4 at 58,068,886 bp
  • G to A, chromosome 4 at 103,319,374 bp
  • T to A, chromosome 5 at 4,043,948 bp
  • A to G, chromosome 5 at 75,181,083 bp
  • G to A, chromosome 5 at 100,446,560 bp
  • T to A, chromosome 5 at 145,244,697 bp
  • G to A, chromosome 6 at 17,097,408 bp
  • A to C, chromosome 6 at 42,626,437 bp
  • C to T, chromosome 6 at 47,890,633 bp
  • A to G, chromosome 6 at 48,978,167 bp
  • A to G, chromosome 6 at 57,925,929 bp
  • G to T, chromosome 6 at 66,777,032 bp
  • A to T, chromosome 6 at 71,399,125 bp
  • T to A, chromosome 6 at 142,455,141 bp
  • G to T, chromosome 7 at 86,148,141 bp
  • G to A, chromosome 7 at 89,632,900 bp
  • T to C, chromosome 7 at 108,081,549 bp
  • C to T, chromosome 7 at 126,412,109 bp
  • T to C, chromosome 8 at 122,571,756 bp
  • T to C, chromosome 9 at 9,011,432 bp
  • C to T, chromosome 9 at 58,857,798 bp
  • T to C, chromosome 10 at 60,534,423 bp
  • G to A, chromosome 10 at 103,409,787 bp
  • T to C, chromosome 11 at 48,907,920 bp
  • T to C, chromosome 11 at 48,908,094 bp
  • T to C, chromosome 11 at 83,769,672 bp
  • C to A, chromosome 11 at 98,599,033 bp
  • C to T, chromosome 12 at 16,553,962 bp
  • T to C, chromosome 12 at 35,144,220 bp
  • T to A, chromosome 12 at 111,539,884 bp
  • A to G, chromosome 13 at 94,565,735 bp
  • A to C, chromosome 16 at 32,753,917 bp
  • C to A, chromosome 16 at 33,551,033 bp
  • C to T, chromosome 16 at 93,892,755 bp
  • T to A, chromosome 16 at 94,466,877 bp
  • A to G, chromosome 17 at 25,750,724 bp
  • A to G, chromosome 17 at 49,070,536 bp
  • A to G, chromosome 18 at 25,110,221 bp
  • A to C, chromosome 18 at 70,483,534 bp
  • A to G, chromosome 19 at 37,685,779 bp
  • G to A, chromosome X at 53,293,499 bp
  • A to G, chromosome Y at 84,120,684 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4532 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041772-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.