Strain Name:
C57BL/6J-MtgxR4538Btlr/Mmmh
Stock Number:
041775-MU
Citation ID:
RRID:MMRRC_041775-MU
Other Names:
R4538 (G1), C57BL/6J-MtgxR4538Btlr
Major Collection:

Gene Information

Lrrn1
Name: leucine rich repeat protein 1, neuronal
Synonyms: NLRR-1, 2810047E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16979
Homologene: 32036
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 271639
Homologene: 10188
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 277854
Homologene: 34718
Chd4
Name: chromodomain helicase DNA binding protein 4
Synonyms: D6Ertd380e, 9530019N15Rik, Mi-2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 107932
HGNC: HGNC:1919
Homologene: 68175
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Strada
Name: STE20-related kinase adaptor alpha
Synonyms: 6030402H20Rik, 2610019A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72149
Homologene: 12448
Washc3
Name: WASH complex subunit 3
Synonyms: 5730495F03Rik, 2900091E11Rik, Ccdc53
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67282
Homologene: 9364
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235567
Homologene: 13574
Ebf1
Name: early B cell factor 1
Synonyms: Olf-1, O/E-1, Olf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13591
HGNC: HGNC:3126
Homologene: 7297
Cyp7b1
Name: cytochrome P450, family 7, subfamily b, polypeptide 1
Synonyms: hct-1, D3Ertd552e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13123
HGNC: HGNC:2652
Homologene: 3544
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Kng2
Name: kininogen 2
Synonyms: Kininogen-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 385643
HGNC: HGNC:6383
Homologene: 88343
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: LOC381663, A330009G12Rik, Sec63d1, Mer3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330149
Homologene: 87103
Kif1a
Name: kinesin family member 1A
Synonyms: Kns1, ATSV, N-3 kinesin, LOC381283, C630002N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
B4galt3
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3
Synonyms: beta4GalT-III, ESTM26, 9530061M23Rik, R74981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 57370
HGNC: HGNC:926
Homologene: 48241
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20957
Homologene: 2389
Egflam
Name: EGF-like, fibronectin type III and laminin G domains
Synonyms: nectican, pikachurin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268780
Homologene: 65044
Cpd
Name: carboxypeptidase D
Synonyms: D830034L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12874
HGNC: HGNC:2301
Homologene: 999
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64452
VEGA: 10
Ripk4
Name: receptor-interacting serine-threonine kinase 4
Synonyms: PKK, DIk, RIP4, ANKK2, Ankrd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 72388
HGNC: HGNC:496
Homologene: 10772
Slc9a3
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3
Synonyms: NHE3, 9030624O13Rik, NHE-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105243
VEGA: 13
Homologene: 55804
Mul1
Name: mitochondrial ubiquitin ligase activator of NFKB 1
Synonyms: 0610009K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68350
Homologene: 11576
Itprid1
Name: ITPR interacting domain containing 1
Synonyms: D530004J12Rik, Ccdc129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232016
Homologene: 52344
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Men1
Name: multiple endocrine neoplasia 1
Synonyms: menin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17283
HGNC: HGNC:7010
Homologene: 7418
Ifih1
Name: interferon induced with helicase C domain 1
Synonyms: Helicard, MDA5, MDA-5, 9130009C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71586
Homologene: 32535
Olfr168
Name: olfactory receptor 168
Synonyms: GA_x54KRFPKG5P-15979009-15978071, MOR271-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258354
Homologene: 128057
Cope
Name: coatomer protein complex, subunit epsilon
Synonyms: Cope1, 1110005D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 59042
HGNC: HGNC:2234
Homologene: 5254
3110018I06Rik
Name: RIKEN cDNA 3110018I06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Pramel6
Name: PRAME like 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 347711
Homologene: 77635
Trbv17
Name: T cell receptor beta, variable 17
Synonyms: Tcrb-V9, Gm16779
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100124681
RP23-330I2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 93,077,047 bp
  • A to T, chromosome 1 at 165,513,127 bp
  • T to C, chromosome 1 at 171,272,710 bp
  • A to G, chromosome 2 at 62,617,412 bp
  • T to G, chromosome 2 at 87,508,559 bp
  • A to T, chromosome 3 at 18,097,581 bp
  • A to T, chromosome 3 at 102,840,962 bp
  • T to A, chromosome 4 at 32,722,334 bp
  • T to C, chromosome 4 at 138,438,395 bp
  • T to C, chromosome 5 at 32,983,946 bp
  • T to C, chromosome 5 at 106,874,890 bp
  • A to C, chromosome 6 at 41,163,352 bp
  • A to G, chromosome 6 at 55,897,801 bp
  • T to A, chromosome 6 at 107,568,637 bp
  • A to T, chromosome 6 at 125,120,686 bp
  • A to G, chromosome 6 at 142,614,412 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • T to A, chromosome 8 at 70,306,507 bp
  • CT to C, chromosome 9 at 104,186,805 bp
  • C to T, chromosome 10 at 76,178,095 bp
  • T to A, chromosome 10 at 88,216,009 bp
  • T to A, chromosome 11 at 44,907,995 bp
  • A to T, chromosome 11 at 76,790,999 bp
  • A to C, chromosome 11 at 106,167,825 bp
  • G to A, chromosome 12 at 107,488,837 bp
  • T to C, chromosome 13 at 74,161,732 bp
  • T to C, chromosome 15 at 7,252,437 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to T, chromosome 16 at 19,530,631 bp
  • C to A, chromosome 16 at 22,988,063 bp
  • A to T, chromosome 16 at 97,743,152 bp
  • T to C, chromosome 19 at 6,336,754 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4538 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041775-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.