Strain Name:
C57BL/6J-MtgxR4540Btlr/Mmmh
Stock Number:
041776-MU
Citation ID:
RRID:MMRRC_041776-MU
Other Names:
R4540 (G1), C57BL/6J-MtgxR4540Btlr
Major Collection:

Gene Information

Cops3
Name: COP9 signalosome subunit 3
Synonyms: Csn3, Sgn3, COP9 complex S3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 26572
HGNC: HGNC:2239
Homologene: 2710
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: 6720416K24Rik, Gtl6, 1110036I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14897
Homologene: 44226
Nup107
Name: nucleoporin 107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 103468
VEGA: 10
Homologene: 5555
Arrdc3
Name: arrestin domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 105171
Homologene: 69318
Bbs9
Name: Bardet-Biedl syndrome 9 (human)
Synonyms: EST 3159894, E130103I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 319845
Homologene: 44480
Nlrp3
Name: NLR family, pyrin domain containing 3
Synonyms: cryopyrin, Mmig1, NALP3, Cias1, Pypaf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216799
Homologene: 3600
Rae1
Name: ribonucleic acid export 1
Synonyms: D2Ertd342e, MNRP41, MNRP, 41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66679
HGNC: HGNC:9828
Homologene: 2676
Supt3
Name: SPT3, SAGA and STAGA complex component
Synonyms: SPT3L, 2310066G22Rik, SPT3, Supt3h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 109115
Homologene: 121570
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74499
Homologene: 11542
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Mag
Name: myelin-associated glycoprotein
Synonyms: siglec-4a, Gma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17136
HGNC: HGNC:6783
Homologene: 1771
Echdc1
Name: enoyl Coenzyme A hydratase domain containing 1
Synonyms: 1700028A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52665
Homologene: 23106
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241516
Homologene: 110349
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: A330009G12Rik, LOC381663, Sec63d1, Mer3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330149
Homologene: 87103
Nadsyn1
Name: NAD synthetase 1
Synonyms: 9130012B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 78914
Homologene: 6098
Ihh
Name: Indian hedgehog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16147
HGNC: HGNC:5956
Homologene: 22586
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 81877
Homologene: 49589
Iba57
Name: IBA57 homolog, iron-sulfur cluster assembly
Synonyms: 4930543L23Rik, A230051G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216792
Homologene: 13844
Cep162
Name: centrosomal protein 162
Synonyms: 4922501C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 382090
Homologene: 8930
Pitrm1
Name: pitrilysin metallepetidase 1
Synonyms: PreP, Ntup1, 2310012C15Rik, MP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 69617
VEGA: 13
Homologene: 5742
Cntn4
Name: contactin 4
Synonyms: Axcam, BIG-2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269784
HGNC: HGNC:2174
Homologene: 14257
Adam6a
Name: a disintegrin and metallopeptidase domain 6A
Synonyms: Adam6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238406
HGNC: HGNC:213
Homologene: 128362
Ccdc51
Name: coiled-coil domain containing 51
Synonyms: 5730568A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66658
Homologene: 11646
Lhcgr
Name: luteinizing hormone/choriogonadotropin receptor
Synonyms: LH-R, Lhr, Gpcr19-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 16867
VEGA: 17
HGNC: HGNC:6585
Homologene: 37276
Spag17
Name: sperm associated antigen 17
Synonyms: 4931427F14Rik, PF6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 74362
Homologene: 52601
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Kcnh7
Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
Synonyms: 9330137I11Rik, Kv11.3, erg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170738
Homologene: 13249
Lrrtm2
Name: leucine rich repeat transmembrane neuronal 2
Synonyms: C630011A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 107065
VEGA: 18
Homologene: 65342
Pth2r
Name: parathyroid hormone 2 receptor
Synonyms: Pthr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 213527
HGNC: HGNC:9609
Homologene: 3701
Kndc1
Name: kinase non-catalytic C-lobe domain (KIND) containing 1
Synonyms: very-kind, 2410012C07Rik, B830014K08Rik, VKIND
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 76484
Homologene: 45138
Cd1d1
Name: CD1d1 antigen
Synonyms: CD1.1, Cd1d, Cd1a, Ly-38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12479
HGNC: HGNC:1637
Homologene: 1337
Hcfc2
Name: host cell factor C2
Synonyms: fkls, 1700129L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67933
Homologene: 8337
Gm4353
Name: predicted gene 4353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100043313
VEGA: 7
Selenoi
Name: selenoprotein I
Synonyms: D5Wsu178e, C79563, 4933402G07Rik, Ept1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 28042
Homologene: 100935
Desi1
Name: desumoylating isopeptidase 1
Synonyms: D15Wsu75e, Pppde2, Fam152b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 28075
Homologene: 15891
Olfr140
Name: olfactory receptor 140
Synonyms: MOR235-1, A16, GA_x6K02T2Q125-51484508-51483600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 57272
Homologene: 41379
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Tbc1d30
Name: TBC1 domain family, member 30
Synonyms: 4930505D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 74694
VEGA: 10
Homologene: 18930
Olfr1305
Name: olfactory receptor 1305
Synonyms: GA_x6K02T2Q125-72930843-72929905, MOR245-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258396
Homologene: 74056
AC120402.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
Gm26904
Name: predicted gene, 26904
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 65,282,201 bp
  • T to A, chromosome 1 at 74,948,399 bp
  • A to G, chromosome 1 at 84,749,276 bp
  • A to G, chromosome 2 at 62,739,186 bp
  • A to T, chromosome 2 at 82,951,665 bp
  • T to C, chromosome 2 at 90,052,150 bp
  • T to C, chromosome 2 at 111,873,201 bp
  • G to T, chromosome 2 at 173,015,392 bp
  • A to G, chromosome 3 at 86,996,705 bp
  • C to T, chromosome 3 at 100,088,381 bp
  • A to G, chromosome 3 at 114,097,166 bp
  • A to G, chromosome 5 at 30,256,087 bp
  • A to C, chromosome 5 at 106,874,221 bp
  • A to G, chromosome 6 at 39,644,333 bp
  • A to G, chromosome 6 at 106,675,748 bp
  • A to C, chromosome 7 at 30,900,729 bp
  • A to G, chromosome 7 at 116,083,977 bp
  • C to A, chromosome 7 at 139,921,427 bp
  • A to G, chromosome 7 at 143,803,223 bp
  • T to C, chromosome 9 at 22,655,247 bp
  • T to C, chromosome 9 at 87,212,939 bp
  • T to C, chromosome 9 at 109,092,220 bp
  • G to A, chromosome 10 at 29,344,582 bp
  • T to C, chromosome 10 at 81,315,094 bp
  • G to C, chromosome 10 at 82,732,647 bp
  • T to C, chromosome 10 at 117,762,020 bp
  • T to C, chromosome 10 at 121,279,158 bp
  • G to A, chromosome 11 at 59,163,078 bp
  • G to A, chromosome 11 at 59,551,899 bp
  • A to T, chromosome 11 at 59,830,154 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • G to A, chromosome 12 at 84,417,659 bp
  • A to T, chromosome 12 at 113,544,499 bp
  • T to A, chromosome 13 at 6,555,470 bp
  • C to A, chromosome 13 at 80,890,671 bp
  • A to T, chromosome 15 at 81,999,585 bp
  • C to T, chromosome 17 at 25,246,670 bp
  • C to T, chromosome 17 at 34,703,335 bp
  • T to C, chromosome 17 at 45,036,775 bp
  • C to T, chromosome 17 at 46,503,089 bp
  • A to G, chromosome 17 at 88,755,608 bp
  • T to A, chromosome 18 at 35,213,146 bp
  • A to C, chromosome 18 at 36,931,627 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4540 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041776-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.