Strain Name:
C57BL/6J-MtgxR4541Btlr/Mmmh
Stock Number:
041777-MU
Citation ID:
RRID:MMRRC_041777-MU
Other Names:
R4541 (G1), C57BL/6J-MtgxR4541Btlr
Major Collection:

Strain Information

B4galt6
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 56386
VEGA: 18
HGNC: HGNC:929
Homologene: 3506
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22359
Homologene: 443
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66865
Homologene: 6078
Chst5
Name: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56773
Homologene: 56927
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, mBSC2, Nkcc1, sy-ns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20496
VEGA: 18
Homologene: 20283
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Prkcq
Name: protein kinase C, theta
Synonyms: PKC-0, Pkcq, A130035A12Rik, PKC-theta, PKC theta, PKCtheta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18761
HGNC: HGNC:9410
Homologene: 21263
Tcea1
Name: transcription elongation factor A (SII) 1
Synonyms: S-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21399
Homologene: 55984
2810021J22Rik
Name: RIKEN cDNA 2810021J22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69944
Homologene: 138400
Napb
Name: N-ethylmaleimide sensitive fusion protein attachment protein beta
Synonyms: SNARE, b-SNAP, I47, E161, Brp14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17957
Homologene: 5332
Glul
Name: glutamate-ammonia ligase (glutamine synthetase)
Synonyms: Glns, GS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14645
HGNC: HGNC:4341
Homologene: 37567
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74499
Homologene: 11542
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Cd8a
Name: CD8 antigen, alpha chain
Synonyms: Ly-B, Ly-2, Ly-35, Lyt-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12525
HGNC: HGNC:1706
Homologene: 133777
Clic5
Name: chloride intracellular channel 5
Synonyms: 5730531E12Rik, nmf318
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224796
VEGA: 17
Homologene: 987
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Klhl14
Name: kelch-like 14
Synonyms: 6330403N15Rik, printor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225266
Homologene: 28121
Or10g9b
Name: olfactory receptor family 10 subfamily G member 9B
Synonyms: GA_x6K02T2PVTD-33705428-33704496, MOR223-2, Olfr980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 259110
VEGA: 9
Homologene: 81557
Rnf225
Name: ring finger protein 225
Synonyms: 2310014L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381845
Homologene: 78604
Ceacam12
Name: CEA cell adhesion molecule 12
Synonyms: Ceacam12-C3, Ceacam12-C1, 1600031J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67315
Homologene: 137375
Ddhd1
Name: DDHD domain containing 1
Synonyms: 9630061G18Rik, 4921528E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 114874
Homologene: 35221
Slc36a1
Name: solute carrier family 36 (proton/amino acid symporter), member 1
Synonyms: Pat1, 5830411H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 215335
Homologene: 121860
Itgad
Name: integrin, alpha D
Synonyms: Cd11d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381924
HGNC: HGNC:6146
Homologene: 56919
Tbc1d2b
Name: TBC1 domain family, member 2B
Synonyms: 1810061M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67016
Homologene: 56694
Evpl
Name: envoplakin
Synonyms: 210kDa protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14027
HGNC: HGNC:3503
Homologene: 1506
Mrps2
Name: mitochondrial ribosomal protein S2
Synonyms: 1500019M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 118451
Homologene: 99837
Vsig10
Name: V-set and immunoglobulin domain containing 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231668
Homologene: 10414
Pla2r1
Name: phospholipase A2 receptor 1
Synonyms: PLA2-I receptor, M-type receptor, Pla2g1br
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18779
HGNC: HGNC:9042
Homologene: 32016
Or4f15
Name: olfactory receptor family 4 subfamily F member 15
Synonyms: GA_x6K02T2Q125-73031456-73030518, MOR245-5, Olfr1309
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258439
Homologene: 74054
Atxn7l1
Name: ataxin 7-like 1
Synonyms: 2810423G08Rik, Atxn7l4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380753
Homologene: 53366
Cfap43
Name: cilia and flagella associated protein 43
Synonyms: 4930428C11Rik, 4632415N18Rik, 4930463G05Rik, D19Ertd652e, Wdr96
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 100048534
Homologene: 36425
Zfp974
Name: zinc finger protein 974
Synonyms: 1700049G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73430
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: 9230101H05Rik, Miwi2, MIWI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330890
Homologene: 45122
Kcnk10
Name: potassium channel, subfamily K, member 10
Synonyms: 3010005K24Rik, 1700024D23Rik, Trek2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 72258
VEGA: 12
HGNC: HGNC:6273
Homologene: 11321
Tubgcp4
Name: tubulin, gamma complex associated protein 4
Synonyms: 4932441P04Rik, D2Ertd435e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51885
Homologene: 8690
Tomm34
Name: translocase of outer mitochondrial membrane 34
Synonyms: TOM34, 2610100K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67145
Homologene: 4956
Dbpht2
Name: DNA binding protein with his-thr domain
Synonyms: Spot2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 386753
VEGA: 12
Vmn1r42
Name: vomeronasal 1 receptor 42
Synonyms: V1ra6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113848
Homologene: 130651
Tlcd4
Name: TLC domain containing 4
Synonyms: C730036B01Rik, 4930577M16Rik, Tmem56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99887
Homologene: 45107
Cdca7l
Name: cell division cycle associated 7 like
Synonyms: JPO2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217946
Homologene: 10285
Tbc1d10c
Name: TBC1 domain family, member 10c
Synonyms: 1810062O14Rik, Carabin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 108995
VEGA: 19
Homologene: 77854
Mymx
Name: myomixer, myoblast fusion factor
Synonyms: minion, myomerger
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 653016
Nlrp1c-ps
Name: NLR family, pyrin domain containing 1C, pseudogene
Synonyms: Nalp1c, OTTMUSG00000006090
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 627984
Ccnb3
Name: cyclin B3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 209091
Homologene: 13199
Sco1
Name: SCO1 cytochrome c oxidase assembly protein
Synonyms: 2610001C07Rik, D11Bwg1310e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52892
Homologene: 3374
4732463B04Rik
Name: RIKEN cDNA 4732463B04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
RP23-302N11.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,893,436 bp
  • T to A, chromosome 1 at 153,903,036 bp
  • T to C, chromosome 1 at 172,118,957 bp
  • G to T, chromosome 2 at 11,283,812 bp
  • T to G, chromosome 2 at 26,390,189 bp
  • G to T, chromosome 2 at 28,468,400 bp
  • C to T, chromosome 2 at 60,427,738 bp
  • A to G, chromosome 2 at 111,983,636 bp
  • A to T, chromosome 2 at 121,195,426 bp
  • G to A, chromosome 2 at 148,709,309 bp
  • A to G, chromosome 2 at 164,054,799 bp
  • A to G, chromosome 3 at 121,235,235 bp
  • C to T, chromosome 5 at 117,352,816 bp
  • A to T, chromosome 6 at 71,373,872 bp
  • A to T, chromosome 6 at 89,845,551 bp
  • T to C, chromosome 7 at 12,928,593 bp
  • G to A, chromosome 7 at 18,071,723 bp
  • C to G, chromosome 7 at 27,926,404 bp
  • G to A, chromosome 7 at 125,318,578 bp
  • A to T, chromosome 7 at 128,198,115 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • T to C, chromosome 8 at 111,914,592 bp
  • C to A, chromosome 9 at 14,718,316 bp
  • A to C, chromosome 9 at 40,006,293 bp
  • A to T, chromosome 9 at 90,205,169 bp
  • T to C, chromosome 11 at 55,222,023 bp
  • C to T, chromosome 11 at 58,878,850 bp
  • G to T, chromosome 11 at 67,052,842 bp
  • A to G, chromosome 11 at 71,280,880 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • T to G, chromosome 11 at 116,232,644 bp
  • C to A, chromosome 12 at 33,363,003 bp
  • A to T, chromosome 12 at 74,299,160 bp
  • A to T, chromosome 12 at 84,043,248 bp
  • A to G, chromosome 12 at 98,436,277 bp
  • T to C, chromosome 12 at 117,872,363 bp
  • G to A, chromosome 14 at 45,622,856 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to T, chromosome 17 at 25,243,737 bp
  • C to T, chromosome 17 at 44,242,069 bp
  • GCC to GC, chromosome 17 at 45,601,593 bp
  • A to G, chromosome 18 at 20,745,439 bp
  • A to T, chromosome 18 at 21,554,639 bp
  • T to A, chromosome 18 at 57,912,965 bp
  • G to T, chromosome 19 at 4,189,474 bp
  • T to C, chromosome 19 at 27,238,792 bp
  • C to T, chromosome 19 at 47,748,015 bp
  • T to C, chromosome X at 7,009,069 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4541 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041777-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.