Strain Name:
C57BL/6J-MtgxR4546Btlr/Mmmh
Stock Number:
041780-MU
Citation ID:
RRID:MMRRC_041780-MU
Other Names:
R4546 (G1), C57BL/6J-MtgxR4546Btlr
Major Collection:

Strain Information

Vcp
Name: valosin containing protein
Synonyms: AAA ATPase p97, CDC48, p97, p97/VCP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Glrb
Name: glycine receptor, beta subunit
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14658
HGNC: HGNC:4329
Homologene: 20224
Il21r
Name: interleukin 21 receptor
Synonyms: NILR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60504
HGNC: HGNC:6006
Homologene: 11040
Lims1
Name: LIM and senescent cell antigen-like domains 1
Synonyms: 2310016J22Rik, C430041B13Rik, Lims1l, 4921524A02Rik, PINCH1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110829
HGNC: HGNC:6616
Homologene: 68428
Dnajc21
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78244
Homologene: 6752
Cdk13
Name: cyclin dependent kinase 13
Synonyms: Cdc2l5, 2310015O17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Bnc2
Name: basonuclin zinc finger protein 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242509
Homologene: 18243
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Dcaf8
Name: DDB1 and CUL4 associated factor 8
Synonyms: Wdr42a, D1Ucla4, D1Dau35e, H326
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98193
Homologene: 56725
Cenpf
Name: centromere protein F
Synonyms: Lek1, mitosin, 6530404A22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
F2rl3
Name: F2R like thrombin or trypsin receptor 3
Synonyms: PAR4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14065
HGNC: HGNC:3540
Homologene: 36148
Mfn2
Name: mitofusin 2
Synonyms: Fzo, hypertension related protein 1, D630023P19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170731
Homologene: 8915
Ccnyl1
Name: cyclin Y-like 1
Synonyms: 9630037P07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227210
Homologene: 65050
Ift122
Name: intraflagellar transport 122
Synonyms: C86139, sopb, Wdr10
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 81896
Homologene: 12819
Lepr
Name: leptin receptor
Synonyms: OB-RGRP, Leprb, Obr, LEPROT, obese-like, obl, Modb1, leptin receptor gene-related protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16847
HGNC: HGNC:6554
Homologene: 1731
Atf7
Name: activating transcription factor 7
Synonyms: 1110012F10Rik, 9430065F09Rik, C130020M04Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223922
HGNC: HGNC:792
Homologene: 4994
Muc15
Name: mucin 15
Synonyms: D730046L02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269328
Homologene: 51680
Urm1
Name: ubiquitin related modifier 1
Synonyms: 2900073H19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68205
Homologene: 41688
Ncapg
Name: non-SMC condensin I complex, subunit G
Synonyms: Hcapg, MFT.M05.13, 5730507H05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54392
Homologene: 44071
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: PTR4, PHT1, C130069N12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100561
Homologene: 26432
9930012K11Rik
Name: RIKEN cDNA 9930012K11 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268759
Homologene: 19540
Slc7a8
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Synonyms: LAT2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50934
VEGA: 14
Homologene: 8166
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Trpm6
Name: transient receptor potential cation channel, subfamily M, member 6
Synonyms: CHAK2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225997
VEGA: 19
Homologene: 9767
Wdr11
Name: WD repeat domain 11
Synonyms: Wdr11, 2900055P10Rik, Brwd2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207425
Homologene: 41229
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, HL19, LOC380889, Dnahc7l, DLP12, Dnahc12, 4921531P07Rik, DHC3, HL-19
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: Jhdm1d, Kdm7a, A630082K20Rik, ENSMUSG00000073143
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Vmn2r78
Name: vomeronasal 2, receptor 78
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637896
Homologene: 115466
Il5ra
Name: interleukin 5 receptor, alpha
Synonyms: CDw125, IL-5 receptor alpha chain, CD125, Il5r
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16192
HGNC: HGNC:6017
Homologene: 473
Pear1
Name: platelet endothelial aggregation receptor 1
Synonyms: Jedi-1, 3110045G13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73182
Homologene: 12492
Alpi
Name: alkaline phosphatase, intestinal
Synonyms: 2010001C14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76768
Homologene: 122414
Klhl5
Name: kelch-like 5
Synonyms: 1300013C10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71778
HGNC: HGNC:6356
Homologene: 56736
Cspg4
Name: chondroitin sulfate proteoglycan 4
Synonyms: 4732461B14Rik, NG2, AN2, Cspg4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 121021
VEGA: 9
HGNC: HGNC:2466
Homologene: 20445
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: ORP-6, 1110062M20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99031
Homologene: 101447
Mest
Name: mesoderm specific transcript
Synonyms: Peg1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17294
HGNC: HGNC:7028
Homologene: 1800
Dlec1
Name: deleted in lung and esophageal cancer 1
Synonyms: D630005C06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320256
HGNC: HGNC:2899
Homologene: 84733
Vmn2r9
Name: vomeronasal 2, receptor 9
Synonyms: EG435864
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 435864
Homologene: 129606
Pdhx
Name: pyruvate dehydrogenase complex, component X
Synonyms: Pdx1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27402
Homologene: 55757
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Ahcyl
Name: adenosylhomocysteinase like
Synonyms: Gm4737
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11615
VEGA: 16
HGNC: HGNC:343
Rbks
Name: ribokinase
Synonyms: 5230400M11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71336
Homologene: 5482
Cr2
Name: complement receptor 2
Synonyms: CD35, C3DR, Cr-1, Cr-2, Cr1, CD21
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Or10ag59
Name: olfactory receptor family 10 subfamily AG member 59
Synonyms: MOR264-9P, MOR264-23, GA_x6K02T2Q125-49078087-49079031, Olfr1129
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258111
Homologene: 133705
Or1e19
Name: olfactory receptor family 1 subfamily E member 19
Synonyms: MOR135-2, GA_x6K02T2P1NL-3586282-3585338, Olfr378
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259026
Homologene: 74110
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320720
Homologene: 36420
Plod3
Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms: lysyl hydroxylase 3, LH3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26433
HGNC: HGNC:9083
Homologene: 843
Nkd2
Name: naked cuticle 2
Synonyms: 2210403L10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72293
Homologene: 12459
Hdac1-ps
Name: histone deacetylase 1, pseudogene
Synonyms: Gm10093, EG15181
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15181
VEGA: 17
Alg9
Name: ALG9 alpha-1,2-mannosyltransferase
Synonyms: 8230402H15Rik, Dibd1, B430313H07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102580
Homologene: 6756
Nphp1
Name: nephronophthisis 1 (juvenile) homolog (human)
Synonyms: nephrocystin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53885
HGNC: HGNC:7905
Homologene: 229
Or2a12
Name: olfactory receptor family 2 subfamily A member 12
Synonyms: Olfr446, GA_x6K02T2P3E9-4632269-4631343, MOR261-12
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258292
Homologene: 17179
Cfap91
Name: cilia and flagella associated protein 91
Synonyms: Spata26, 4932425I24Rik, Maats1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320214
Homologene: 33470
Asmt
Name: acetylserotonin O-methyltransferase
Synonyms: Hiomt
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 107626
VEGA: X
HGNC: HGNC:750
Tmem200c
Name: transmembrane protein 200C
Synonyms: Gm6338
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 622645
VEGA: 17
Homologene: 78765
Gm5507
Name: predicted gene 5507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 433184
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,723,576 bp
  • T to A, chromosome 1 at 87,099,117 bp
  • T to C, chromosome 1 at 172,179,893 bp
  • A to C, chromosome 1 at 189,654,650 bp
  • A to G, chromosome 1 at 195,171,041 bp
  • A to G, chromosome 2 at 29,842,763 bp
  • C to T, chromosome 2 at 69,712,311 bp
  • T to C, chromosome 2 at 76,584,492 bp
  • T to C, chromosome 2 at 76,822,588 bp
  • T to C, chromosome 2 at 87,575,186 bp
  • A to T, chromosome 2 at 103,073,397 bp
  • T to C, chromosome 2 at 110,737,499 bp
  • C to T, chromosome 2 at 127,766,019 bp
  • T to C, chromosome 3 at 80,879,686 bp
  • C to T, chromosome 3 at 87,754,661 bp
  • T to C, chromosome 4 at 42,988,813 bp
  • C to G, chromosome 4 at 51,229,651 bp
  • A to T, chromosome 4 at 51,229,840 bp
  • A to G, chromosome 4 at 84,291,976 bp
  • T to A, chromosome 4 at 101,814,641 bp
  • A to G, chromosome 4 at 147,887,452 bp
  • G to A, chromosome 5 at 17,694,772 bp
  • T to C, chromosome 5 at 31,624,568 bp
  • T to C, chromosome 5 at 45,671,212 bp
  • C to T, chromosome 5 at 65,164,690 bp
  • T to G, chromosome 5 at 108,847,685 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • T to A, chromosome 5 at 136,988,947 bp
  • C to G, chromosome 5 at 137,383,834 bp
  • T to C, chromosome 6 at 30,740,680 bp
  • T to C, chromosome 6 at 39,175,472 bp
  • C to T, chromosome 6 at 42,927,414 bp
  • G to T, chromosome 6 at 106,738,498 bp
  • T to A, chromosome 6 at 115,890,588 bp
  • T to C, chromosome 7 at 86,954,603 bp
  • A to G, chromosome 7 at 125,628,899 bp
  • A to G, chromosome 7 at 129,629,005 bp
  • C to T, chromosome 8 at 72,762,583 bp
  • C to T, chromosome 9 at 50,805,354 bp
  • T to A, chromosome 9 at 56,888,629 bp
  • A to T, chromosome 9 at 95,862,895 bp
  • A to G, chromosome 9 at 118,556,845 bp
  • A to T, chromosome 9 at 119,128,078 bp
  • T to C, chromosome 10 at 58,418,790 bp
  • T to C, chromosome 11 at 73,425,186 bp
  • T to C, chromosome 13 at 17,766,574 bp
  • T to C, chromosome 13 at 73,823,356 bp
  • A to T, chromosome 14 at 26,773,014 bp
  • C to G, chromosome 14 at 54,735,790 bp
  • G to A, chromosome 14 at 70,156,478 bp
  • T to C, chromosome 15 at 10,447,097 bp
  • T to C, chromosome 15 at 76,181,557 bp
  • A to G, chromosome 15 at 102,534,327 bp
  • C to T, chromosome 16 at 38,335,523 bp
  • C to T, chromosome 16 at 46,153,967 bp
  • A to G, chromosome 17 at 68,842,171 bp
  • A to T, chromosome 17 at 78,492,959 bp
  • T to A, chromosome 18 at 53,984,337 bp
  • A to G, chromosome 19 at 18,832,477 bp
  • T to C, chromosome X at 170,676,495 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4546 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041780-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.