Strain Name:
C57BL/6J-MtgxR4551Btlr/Mmmh
Stock Number:
041782-MU
Citation ID:
RRID:MMRRC_041782-MU
Other Names:
R4551 (G1), C57BL/6J-MtgxR4551Btlr
Major Collection:

Gene Information

Lhx3
Name: LIM homeobox protein 3
Synonyms: P-LIM, mLim-3, Lim3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16871
HGNC: HGNC:6595
Homologene: 7814
Pxn
Name: paxillin
Synonyms: Pax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19303
HGNC: HGNC:9718
Homologene: 37697
Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: BTF2, D5Ertd679e, 5033417D07Rik, 34kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Ptpn5
Name: protein tyrosine phosphatase, non-receptor type 5
Synonyms: Step
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 19259
HGNC: HGNC:9657
Homologene: 8423
Abhd17b
Name: abhydrolase domain containing 17B
Synonyms: Fam108b, 5730446C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226016
VEGA: 19
Homologene: 41089
Psma2
Name: proteasome (prosome, macropain) subunit, alpha type 2
Synonyms: Lmpc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19166
VEGA: 13
HGNC: HGNC:9531
Homologene: 2081
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, Smarcf1, 1110030E03Rik, BAF250a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 93760
Homologene: 21216
Kat6b
Name: K(lysine) acetyltransferase 6B
Synonyms: Myst4, Morf, B130044K16Rik, monocytic leukemia, qkf, querkopf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 54169
Homologene: 136480
Trappc8
Name: trafficking protein particle complex 8
Synonyms: Trs85, D030074E01Rik, 5033403J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 75964
VEGA: 18
Homologene: 40993
Cog6
Name: component of oligomeric golgi complex 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 67542
Homologene: 10802
Cox20
Name: cytochrome c oxidase assembly protein 20
Synonyms: Fam36a, 2310005N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66359
Homologene: 11956
Zfp180
Name: zinc finger protein 180
Synonyms: D130011P11, 2310040I01Rik, HHZ168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 210135
Homologene: 8306
Epgn
Name: epithelial mitogen
Synonyms: 2310069M11Rik, epigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71920
Homologene: 19527
Pprc1
Name: peroxisome proliferative activated receptor, gamma, coactivator-related 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226169
Homologene: 9006
Pgap2
Name: post-GPI attachment to proteins 2
Synonyms: Frag1, 1810006G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233575
Homologene: 137653
Serinc4
Name: serine incorporator 4
Synonyms: A930015D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 574418
Homologene: 67424
Pcdhb16
Name: protocadherin beta 16
Synonyms: Pcdhb8, PcdhbP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93887
HGNC: HGNC:8693
Homologene: 99843
Tab2
Name: TGF-beta activated kinase 1/MAP3K7 binding protein 2
Synonyms: Tak1 binding protein 2, A530078N03Rik, 1110030N06Rik, Map3k7ip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68652
Homologene: 9019
Depdc1a
Name: DEP domain containing 1a
Synonyms: 5830484J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76131
Homologene: 9834
Myo1g
Name: myosin IG
Synonyms: E430002D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 246177
Homologene: 27996
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: PKHDL1, fibrocystin L, D86 mRNA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 192190
Homologene: 16332
Grk2
Name: G protein-coupled receptor kinase 2
Synonyms: Adrbk1, beta-adrenergic receptor kinase-1, Bark-1, betaARK1, beta ARK, beta ARK1, beta-AR kinase-1, Adrbk-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 110355
HGNC: HGNC:289
Homologene: 1223
Alox15
Name: arachidonate 15-lipoxygenase
Synonyms: L-12LO, 12-LO, Alox12l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11687
HGNC: HGNC:433
Homologene: 44935
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 212167
Homologene: 45504
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Odf4
Name: outer dense fiber of sperm tails 4
Synonyms: Oppo1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 252868
Homologene: 17099
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Farp2
Name: FERM, RhoGEF and pleckstrin domain protein 2
Synonyms: Fir, D030026M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227377
Homologene: 8877
Cpd
Name: carboxypeptidase D
Synonyms: D830034L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12874
HGNC: HGNC:2301
Homologene: 999
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 9130019P20Rik, 1110004O12Rik, 4933432B13Rik, A530027J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 108857
Homologene: 87006
Scd3
Name: stearoyl-coenzyme A desaturase 3
Synonyms: 4930513N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 30049
VEGA: 19
Homologene: 137370
Cpa3
Name: carboxypeptidase A3, mast cell
Synonyms: mMC-CPA, MC-CPA, mast cell carboxypeptidase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12873
HGNC: HGNC:2298
Homologene: 122138
Micalcl
Name: MICAL C-terminal like
Synonyms: Ebitein1, 4921517J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100504195
Homologene: 13149
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627743
Homologene: 129680
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: CYLD1, 2900009M21Rik, C130039D01Rik, 2010013M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Aatk
Name: apoptosis-associated tyrosine kinase
Synonyms: AATYK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11302
HGNC: HGNC:21
Homologene: 74861
Olfr45
Name: olfactory receptor 45
Synonyms: IB6, GA_x6K02T2PBJ9-42837030-42837962, MOR253-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18344
Homologene: 79416
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A3, UT-A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 27411
VEGA: 18
Homologene: 5183
Kif26b
Name: kinesin family member 26B
Synonyms: D230039L06Rik, N-11 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269152
Homologene: 18623
Parpbp
Name: PARP1 binding protein
Synonyms: 4930547N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 75317
Homologene: 49519
Zfp932
Name: zinc finger protein 932
Synonyms: 2310001H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69504
Homologene: 137770
Dab2
Name: disabled 2, mitogen-responsive phosphoprotein
Synonyms: 5730435J12Rik, p96, D15Wsu122e, D630005B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 13132
HGNC: HGNC:2662
Homologene: 1026
Tmem236
Name: transmembrane protein 236
Synonyms: 2010003H20Rik, Fam23a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 625286
Homologene: 46598
Vmn2r7
Name: vomeronasal 2, receptor 7
Synonyms: 4933425M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 319217
Homologene: 129754
Mybphl
Name: myosin binding protein H-like
Synonyms: 1110037P11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68753
Homologene: 19221
Sdr42e1
Name: short chain dehydrogenase/reductase family 42E, member 1
Synonyms: 4632417N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 74032
Homologene: 23654
Gpr45
Name: G protein-coupled receptor 45
Synonyms: 9230112G11Rik, PSP24alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 93690
HGNC: HGNC:4503
Homologene: 5228
Vmn2r81
Name: vomeronasal 2, receptor 81
Synonyms: V2rf2, pheromone recepter, EC1-VR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216144
Homologene: 83483
Papolb
Name: poly (A) polymerase beta (testis specific)
Synonyms: Plap-ps, Papt, Papola-ps, TPAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56522
Homologene: 121622
Ikbke
Name: inhibitor of kappaB kinase epsilon
Synonyms: IKKepsilon, IKK-i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 56489
Homologene: 23168
Dync1i1
Name: dynein cytoplasmic 1 intermediate chain 1
Synonyms: IC74, Dncic1, DIC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 13426
HGNC: HGNC:2963
Homologene: 68398
Armcx5
Name: armadillo repeat containing, X-linked 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 494468
Homologene: 128366
Adgrg7
Name: adhesion G protein-coupled receptor G7
Synonyms: 9130020O16Rik, Gpr128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 239853
Homologene: 13115
C4bp
Name: complement component 4 binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12269
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: prostacyclin receptor, IP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Hus1b
Name: HUS1 checkpoint clamp component B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 210554
Homologene: 51833
Nkx2-4
Name: NK2 homeobox 4
Synonyms: Nkx-2.4, 1700001P03Rik, tinman
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228731
HGNC: HGNC:7837
Homologene: 41526
Olfr657
Name: olfactory receptor 657
Synonyms: MOR40-13, GA_x6K02T2PBJ9-7263864-7264823
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258309
Homologene: 17189
Pdk3
Name: pyruvate dehydrogenase kinase, isoenzyme 3
Synonyms: 2610001M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 236900
HGNC: HGNC:8811
Homologene: 55897
Nlrp5-ps
Name: NLR family, pyrin domain containing 5, pseudogene
Synonyms: Gm18756
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100417675
Mroh9
Name: maestro heat-like repeat family member 9
Synonyms: 4921528O07Rik, Armc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 78258
Homologene: 123521
Olfr360
Name: olfactory receptor 360
Synonyms: GA_x6K02T2NLDC-33760081-33761034, MOR159-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258615
Homologene: 73997
Gm16793
Name: predicted gene, 16793
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 100504714
Man2c1os
Name: mannosidase, alpha, class 2C, member 1, opposite strand
Synonyms: 2410133F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76794
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,032,790 bp
  • T to A, chromosome 1 at 93,618,592 bp
  • A to G, chromosome 1 at 130,636,727 bp
  • T to C, chromosome 1 at 131,258,033 bp
  • A to T, chromosome 1 at 163,044,093 bp
  • A to C, chromosome 1 at 178,322,549 bp
  • T to A, chromosome 1 at 178,884,035 bp
  • A to T, chromosome 2 at 14,219,153 bp
  • G to A, chromosome 2 at 26,201,190 bp
  • G to T, chromosome 2 at 37,068,343 bp
  • A to G, chromosome 2 at 121,453,480 bp
  • C to A, chromosome 2 at 147,084,922 bp
  • T to C, chromosome 3 at 20,219,770 bp
  • T to A, chromosome 3 at 52,998,320 bp
  • T to C, chromosome 3 at 64,690,689 bp
  • T to C, chromosome 3 at 108,374,163 bp
  • T to A, chromosome 3 at 159,522,584 bp
  • A to C, chromosome 4 at 133,695,699 bp
  • A to T, chromosome 5 at 21,290,571 bp
  • A to T, chromosome 5 at 91,027,562 bp
  • T to C, chromosome 5 at 110,009,639 bp
  • G to T, chromosome 5 at 115,552,720 bp
  • A to G, chromosome 5 at 124,590,419 bp
  • T to C, chromosome 5 at 142,529,178 bp
  • T to A, chromosome 6 at 5,923,206 bp
  • A to C, chromosome 7 at 14,561,969 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • A to C, chromosome 7 at 24,104,573 bp
  • C to A, chromosome 7 at 47,090,852 bp
  • C to A, chromosome 7 at 102,226,467 bp
  • A to T, chromosome 7 at 104,636,424 bp
  • T to A, chromosome 7 at 112,381,916 bp
  • A to T, chromosome 7 at 140,691,742 bp
  • G to T, chromosome 8 at 35,588,879 bp
  • A to G, chromosome 8 at 88,707,134 bp
  • T to C, chromosome 8 at 117,663,608 bp
  • T to C, chromosome 9 at 57,131,161 bp
  • G to A, chromosome 10 at 7,907,483 bp
  • T to A, chromosome 10 at 79,268,407 bp
  • T to A, chromosome 10 at 88,093,702 bp
  • A to G, chromosome 11 at 6,517,874 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • T to C, chromosome 11 at 65,841,366 bp
  • C to T, chromosome 11 at 68,922,040 bp
  • T to A, chromosome 11 at 70,344,596 bp
  • C to T, chromosome 11 at 76,811,886 bp
  • T to G, chromosome 11 at 120,011,569 bp
  • A to G, chromosome 13 at 14,616,845 bp
  • C to A, chromosome 13 at 30,947,076 bp
  • A to G, chromosome 14 at 21,661,448 bp
  • G to A, chromosome 15 at 6,435,294 bp
  • C to A, chromosome 15 at 44,550,885 bp
  • A to G, chromosome 16 at 56,748,012 bp
  • A to G, chromosome 17 at 20,226,351 bp
  • T to C, chromosome 18 at 20,874,672 bp
  • T to C, chromosome 18 at 31,985,108 bp
  • C to A, chromosome 18 at 36,655,507 bp
  • T to C, chromosome 18 at 37,479,834 bp
  • A to C, chromosome 18 at 78,195,853 bp
  • C to T, chromosome 19 at 4,286,056 bp
  • T to C, chromosome 19 at 21,680,926 bp
  • C to A, chromosome 19 at 44,215,439 bp
  • A to G, chromosome 19 at 46,067,225 bp
  • A to T, chromosome X at 93,782,240 bp
  • T to A, chromosome X at 135,746,507 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4551 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041782-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.