Strain Name:
C57BL/6J-MtgxR4557Btlr/Mmmh
Stock Number:
041784-MU
Citation ID:
RRID:MMRRC_041784-MU
Other Names:
R4557 (G1), C57BL/6J-MtgxR4557Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: mBSC1, urehr3, D630042G03Rik, Nkcc2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, E330008K23Rik, HALR
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Prdm16
Name: PR domain containing 16
Synonyms: line 27, Mel1, 5730557K01Rik, csp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70673
Homologene: 11139
Bahcc1
Name: BAH domain and coiled-coil containing 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268515
Homologene: 129585
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: CTL2, 1110028E10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Ttc27
Name: tetratricopeptide repeat domain 27
Synonyms: 2610511O17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74196
VEGA: 17
Homologene: 41191
Cep68
Name: centrosomal protein 68
Synonyms: 6030463E10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216543
Homologene: 65235
Mdn1
Name: midasin AAA ATPase 1
Synonyms: 4833432B22Rik, D4Abb1e, LOC213784
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Phrf1
Name: PHD and ring finger domains 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101471
Homologene: 16377
Sft2d2
Name: SFT2 domain containing 2
Synonyms: 2010005O13Rik, cI-45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108735
Homologene: 27069
Exoc1
Name: exocyst complex component 1
Synonyms: A730011E05Rik, Sec3l1, 2810407P21Rik, SEC3, Sec3p
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69940
Homologene: 41241
Zbtb44
Name: zinc finger and BTB domain containing 44
Synonyms: Btbd15, 6030404E16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235132
VEGA: 9
Homologene: 18221
Pitpnm1
Name: phosphatidylinositol transfer protein, membrane-associated 1
Synonyms: RdgB, DRES9
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18739
HGNC: HGNC:9003
Homologene: 3608
Med17
Name: mediator complex subunit 17
Synonyms: Crsp6, Trap80, C330002H14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 234959
VEGA: 9
HGNC: HGNC:2375
Homologene: 3151
Ttn
Name: titin
Synonyms: connectin, 1100001C23Rik, D330041I19Rik, D830007G01Rik, 2310074I15Rik, 2310036G12Rik, mdm, 2310057K23Rik, shru, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: Ptpri, PTPL1, PTP-BL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: Bai3, A830096D10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Ccdc175
Name: coiled-coil domain containing 175
Synonyms: 4930403N07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73936
VEGA: 12
Homologene: 79144
Nalcn
Name: sodium leak channel, non-selective
Synonyms: Vgcnl1, A530023G15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
A830005F24Rik
Name: RIKEN cDNA A830005F24 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 442803
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: mG145, Rp1h, Dcdc3, oxygen-regulated protein 1, Orp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Adamts3
Name: ADAM metallopeptidase with thrombospondin type 1 motif 3
Synonyms: 6330442E02Rik, 1100001H14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Adamts16
Name: ADAM metallopeptidase with thrombospondin type 1 motif 16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 271127
Homologene: 15146
Apba1
Name: amyloid beta precursor protein binding family A member 1
Synonyms: X11alpha, Mint, Lin-10, X11, 6430513E09Rik, Mint1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 319924
VEGA: 19
HGNC: HGNC:578
Homologene: 897
Tmc5
Name: transmembrane channel-like gene family 5
Synonyms: 4932443L08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74424
Homologene: 11713
Or1e29
Name: olfactory receptor family 1 subfamily E member 29
Synonyms: Olfr389, MOR135-6, GA_x6K02T2P1NL-3932085-3931147
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259011
Homologene: 85925
Thap12
Name: THAP domain containing 12
Synonyms: 2900052B10Rik, Dap4, Prkrir
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72981
HGNC: HGNC:9440
Homologene: 37952
Acad11
Name: acyl-Coenzyme A dehydrogenase family, member 11
Synonyms: 5730439E10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102632
Homologene: 49896
Mcrs1
Name: microspherule protein 1
Synonyms: ICP22BP, P78, MSP58, C78274
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 51812
VEGA: 15
HGNC: HGNC:6960
Homologene: 4622
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Or1r1
Name: olfactory receptor family 1 subfamily JRmember 1
Synonyms: Olfr398, MOR157-1, GA_x6K02T2P1NL-4141430-4140486
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258705
HGNC: HGNC:8226
Homologene: 17355
Adamts17
Name: ADAM metallopeptidase with thrombospondin type 1 motif 17
Synonyms: AU023434
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233332
Homologene: 16373
Or6c69
Name: olfactory receptor family 6 subfamily C member 69
Synonyms: Olfr816, GA_x6K02T2PULF-11590830-11589892, MOR113-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258667
Homologene: 133584
Ccdc162
Name: coiled-coil domain containing 162
Synonyms: 5033413D22Rik, Gm6976, Gm29096
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75973
Homologene: 136355
Dlgap1
Name: DLG associated protein 1
Synonyms: Sapap1, SAPAP1, GKAP/SAPAP, 4933422O14Rik, D17Bwg0511e, DAP-1 beta, Gkap
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Smok4a
Name: sperm motility kinase 4A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 272667
Ighv3-6
Name: immunoglobulin heavy variable 3-6
Synonyms: Gm16932
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780829
Gpr149
Name: G protein-coupled receptor 149
Synonyms: Ieda, 9630018L10Rik, PGR10, R35
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229357
Homologene: 16359
Or14j8
Name: olfactory receptor family 14 subfamily J member 8
Synonyms: GA_x6K02T2PSCP-2403971-2403000, Olfr761, MOR218-12, MOR218-6P, MOR218-6P, Olfr1552-ps1, MOR218-5P
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258094
Homologene: 134080
Bcl11a
Name: BCL11 transcription factor A
Synonyms: D930021L15Rik, Evi9a, COUP-TF interacting protein 1, CTIP1, Evi9b, 2810047E18Rik, mouse myeloid leukemia gene, Evi9, Evi9c
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14025
Homologene: 11284
Fbxo4
Name: F-box protein 4
Synonyms: 1700096C12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106052
Homologene: 8134
Snx18
Name: sorting nexin 18
Synonyms: Snag1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 170625
VEGA: 13
Homologene: 14164
AC153941.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Igkv4-78
Name: immunoglobulin kappa variable 4-78
Synonyms: Igkv4-78 immunoglobulin light chain variable region
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545849
Krtap12-20
Name: keratin associated protein 12-20
Synonyms: Gm10100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100502953
VEGA: 10
Homologene: 135713
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,344,663 bp
  • T to C, chromosome 1 at 25,084,279 bp
  • A to G, chromosome 1 at 165,183,984 bp
  • A to T, chromosome 2 at 76,949,015 bp
  • A to G, chromosome 2 at 125,186,641 bp
  • A to G, chromosome 3 at 62,530,870 bp
  • A to C, chromosome 3 at 62,604,497 bp
  • G to T, chromosome 4 at 32,754,437 bp
  • T to A, chromosome 4 at 154,528,827 bp
  • A to T, chromosome 5 at 25,300,315 bp
  • G to A, chromosome 5 at 76,561,443 bp
  • A to G, chromosome 5 at 89,700,487 bp
  • A to G, chromosome 5 at 103,541,110 bp
  • A to G, chromosome 6 at 69,059,771 bp
  • A to T, chromosome 7 at 67,027,893 bp
  • A to G, chromosome 7 at 98,715,845 bp
  • T to C, chromosome 7 at 118,670,733 bp
  • C to T, chromosome 7 at 141,258,929 bp
  • T to G, chromosome 8 at 125,464,415 bp
  • T to C, chromosome 9 at 15,271,697 bp
  • T to G, chromosome 9 at 21,346,783 bp
  • T to G, chromosome 9 at 31,064,248 bp
  • T to C, chromosome 9 at 104,082,839 bp
  • T to A, chromosome 10 at 41,587,388 bp
  • T to C, chromosome 10 at 59,222,472 bp
  • G to A, chromosome 10 at 77,726,531 bp
  • A to G, chromosome 10 at 129,911,529 bp
  • A to T, chromosome 11 at 20,239,113 bp
  • A to G, chromosome 11 at 24,164,004 bp
  • G to A, chromosome 11 at 73,776,481 bp
  • A to T, chromosome 11 at 73,984,599 bp
  • A to G, chromosome 11 at 120,275,088 bp
  • T to A, chromosome 12 at 72,128,306 bp
  • T to C, chromosome 12 at 114,288,198 bp
  • T to C, chromosome 13 at 48,514,461 bp
  • G to A, chromosome 13 at 70,779,518 bp
  • A to G, chromosome 13 at 113,617,828 bp
  • T to C, chromosome 14 at 123,321,235 bp
  • A to G, chromosome 15 at 3,965,705 bp
  • A to G, chromosome 15 at 99,243,147 bp
  • T to C, chromosome 16 at 14,153,977 bp
  • T to C, chromosome 17 at 13,526,381 bp
  • C to A, chromosome 17 at 37,952,251 bp
  • C to A, chromosome 17 at 70,516,689 bp
  • T to G, chromosome 17 at 74,829,549 bp
  • A to G, chromosome 19 at 4,103,085 bp
  • T to A, chromosome 19 at 23,917,592 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4557 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041784-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.