Strain Name:
C57BL/6J-MtgxR4561Btlr/Mmmh
Stock Number:
041786-MU
Citation ID:
RRID:MMRRC_041786-MU
Other Names:
R4561 (G1), C57BL/6J-MtgxR4561Btlr
Major Collection:

Strain Information

Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237877
Homologene: 32611
Cenpc1
Name: centromere protein C1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12617
HGNC: HGNC:1854
Homologene: 1371
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Mfn2
Name: mitofusin 2
Synonyms: hypertension related protein 1, D630023P19Rik, Fzo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170731
Homologene: 8915
Huwe1
Name: HECT, UBA and WWE domain containing 1
Synonyms: Ib772, 5430439H10Rik, LOC382250, Ureb1, C430014N20Rik, Mule, Arf-bp1
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 59026
Homologene: 45994
Cep135
Name: centrosomal protein 135
Synonyms: LOC381644, Cep4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381644
Homologene: 45709
Gbx2
Name: gastrulation brain homeobox 2
Synonyms: MMoxA, Gbx-2, Stra7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14472
HGNC: HGNC:4186
Homologene: 1138
Arnt
Name: aryl hydrocarbon receptor nuclear translocator
Synonyms: Hif1b, ESTM42, D3Ertd557e, bHLHe2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11863
HGNC: HGNC:700
Homologene: 1261
Dera
Name: deoxyribose-phosphate aldolase
Synonyms: 2010002D22Rik, 2500002K03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232449
Homologene: 14562
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74144
Homologene: 10397
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Myof
Name: myoferlin
Synonyms: 2310051D19Rik, E030042N20Rik, Fer1l3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226101
VEGA: 19
HGNC: HGNC:3656
Homologene: 40882
Kcnd2
Name: potassium voltage-gated channel, Shal-related family, member 2
Synonyms: Kv4.2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16508
HGNC: HGNC:6238
Homologene: 40828
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Ppp1r3a
Name: protein phosphatase 1, regulatory subunit 3A
Synonyms: GM, RGL
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 140491
HGNC: HGNC:9291
Homologene: 48124
Srgap3
Name: SLIT-ROBO Rho GTPase activating protein 3
Synonyms: Arhgap14, D130026O08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 259302
Homologene: 56686
Slc24a2
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 2
Synonyms: 6330417K15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76376
Homologene: 10669
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: A630082K20Rik, ENSMUSG00000073143, Kdm7a, Jhdm1d
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 434341
Homologene: 88935
Ttc21b
Name: tetratricopeptide repeat domain 21B
Synonyms: 2410066K11Rik, line 158, Thm1, aln
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 73668
Homologene: 57006
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: MJAM, OATP-D, 5830414C08Rik, Anr1, Slc21a11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108116
Homologene: 40862
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Zfp947
Name: zinc finger protein 947
Synonyms: Gm4769
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210853
Homologene: 133253
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234311
Homologene: 23031
Mest
Name: mesoderm specific transcript
Synonyms: Peg1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17294
HGNC: HGNC:7028
Homologene: 1800
Grk4
Name: G protein-coupled receptor kinase 4
Synonyms: A830025H08Rik, Gprk2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14772
HGNC: HGNC:4543
Homologene: 23158
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: mlst-1, Slc21a6, Slc21a10, Oatp1b2, 7330442B20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28253
Homologene: 75119
Slc35g2
Name: solute carrier family 35, member G2
Synonyms: LOC245020, Tmem22
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245020
Homologene: 11893
Ivl
Name: involucrin
Synonyms: 1110019C06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16447
HGNC: HGNC:6187
Homologene: 137207
Hkdc1
Name: hexokinase domain containing 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216019
Homologene: 128937
Slc22a22
Name: solute carrier family 22 (organic cation transporter), member 22
Synonyms: OAT-PG, BC026439
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 210463
Homologene: 18166
Ctnna2
Name: catenin alpha 2
Synonyms: alpha(N)-catenin, alpha N-catenin, Catna, chp, Catna2, catenin (cadherin associated protein), alpha 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12386
HGNC: HGNC:2510
Homologene: 68394
Glyat
Name: glycine-N-acyltransferase
Synonyms: A330009E03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107146
Homologene: 64840
Klhl30
Name: kelch-like 30
Synonyms: 4631423F02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70788
Homologene: 18891
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
Zfp760
Name: zinc finger protein 760
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240034
VEGA: 17
Calr4
Name: calreticulin 4
Synonyms: 4933403L16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108802
Homologene: 86813
Or5g27
Name: olfactory receptor family 5 subfamily G member 27
Synonyms: GA_x6K02T2Q125-47058060-47059004, MOR175-4, Olfr996
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258429
Homologene: 85934
Tcte2
Name: t-complex-associated testis expressed 2
Synonyms: Tcte-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21646
Gm26919
Name: predicted gene, 26919
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Homologene: 134227
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 11,184,545 bp
  • A to T, chromosome 1 at 20,534,719 bp
  • G to A, chromosome 1 at 68,343,921 bp
  • A to T, chromosome 1 at 89,928,959 bp
  • A to T, chromosome 1 at 91,361,031 bp
  • A to T, chromosome 2 at 52,286,155 bp
  • T to C, chromosome 2 at 66,186,218 bp
  • G to T, chromosome 2 at 85,579,620 bp
  • CCTGCTGCTGCT to CCTGCTGCTGCTGCT, chromosome 3 at 92,571,955 bp
  • A to G, chromosome 3 at 95,452,613 bp
  • A to T, chromosome 4 at 87,227,397 bp
  • A to G, chromosome 4 at 109,246,182 bp
  • C to A, chromosome 4 at 147,877,035 bp
  • C to A, chromosome 5 at 34,694,813 bp
  • C to T, chromosome 5 at 76,638,193 bp
  • C to A, chromosome 5 at 86,047,632 bp
  • A to G, chromosome 6 at 14,754,682 bp
  • A to G, chromosome 6 at 21,216,396 bp
  • A to G, chromosome 6 at 30,732,627 bp
  • C to T, chromosome 6 at 39,152,823 bp
  • A to T, chromosome 6 at 48,475,534 bp
  • A to G, chromosome 6 at 77,636,713 bp
  • A to G, chromosome 6 at 112,781,054 bp
  • A to T, chromosome 6 at 130,677,381 bp
  • A to T, chromosome 6 at 137,780,738 bp
  • A to G, chromosome 6 at 141,671,167 bp
  • G to A, chromosome 7 at 74,504,256 bp
  • T to A, chromosome 7 at 81,308,820 bp
  • T to C, chromosome 8 at 61,942,461 bp
  • A to G, chromosome 8 at 94,477,146 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • C to A, chromosome 9 at 100,553,234 bp
  • A to G, chromosome 9 at 110,052,371 bp
  • T to C, chromosome 10 at 62,409,839 bp
  • T to C, chromosome 10 at 70,002,018 bp
  • A to G, chromosome 11 at 80,095,889 bp
  • T to A, chromosome 14 at 121,559,007 bp
  • T to A, chromosome 15 at 57,263,385 bp
  • A to T, chromosome 15 at 95,094,796 bp
  • T to C, chromosome 17 at 13,722,602 bp
  • T to A, chromosome 17 at 21,723,667 bp
  • G to T, chromosome 17 at 22,146,143 bp
  • G to A, chromosome 17 at 25,742,934 bp
  • A to T, chromosome 18 at 82,620,406 bp
  • G to T, chromosome 19 at 12,651,280 bp
  • T to C, chromosome 19 at 37,922,990 bp
  • A to G, chromosome 19 at 44,835,963 bp
  • A to T, chromosome X at 151,863,959 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4561 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041786-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.