Strain Name:
C57BL/6J-MtgxR4563Btlr/Mmmh
Stock Number:
041788-MU
Citation ID:
RRID:MMRRC_041788-MU
Other Names:
R4563 (G1), C57BL/6J-MtgxR4563Btlr
Major Collection:

Strain Information

Nek6
Name: NIMA (never in mitosis gene a)-related expressed kinase 6
Synonyms: 1300007C09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59126
HGNC: HGNC:7749
Homologene: 49379
Nup93
Name: nucleoporin 93
Synonyms: 2410008G02Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71805
Homologene: 40971
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: p1.m, Mcmd, P1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Pex14
Name: peroxisomal biogenesis factor 14
Synonyms: Pex14p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56273
HGNC: HGNC:8856
Homologene: 37936
Cln3
Name: CLN3 lysosomal/endosomal transmembrane protein, battenin
Synonyms: battenin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12752
HGNC: HGNC:2074
Homologene: 37259
Huwe1
Name: HECT, UBA and WWE domain containing 1
Synonyms: 5430439H10Rik, C430014N20Rik, LOC382250, Ib772, Mule, Arf-bp1, Ureb1
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 59026
Homologene: 45994
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Mgat4a
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme A
Synonyms: 9530018I07Rik, GnT-IVa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269181
HGNC: HGNC:7047
Homologene: 8153
Slc49a4
Name: solute carrier family 49 member 4
Synonyms: RCC4, Dirc2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224132
Homologene: 13137
Mphosph8
Name: M-phase phosphoprotein 8
Synonyms: 1500035L22Rik, 4930548G07Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75339
Homologene: 11380
Pafah1b2
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 2
Synonyms: PAF-AH 30, Pafahb, mus[b], PAF-AH alpha2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18475
VEGA: 9
HGNC: HGNC:8575
Homologene: 1932
Adam17
Name: a disintegrin and metallopeptidase domain 17
Synonyms: Tace, CD156b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11491
HGNC: HGNC:195
Homologene: 2395
Glipr2
Name: GLI pathogenesis-related 2
Synonyms: 5730414A08Rik, GAPR-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 384009
Homologene: 74720
Rprd1a
Name: regulation of nuclear pre-mRNA domain containing 1A
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225283
VEGA: 18
Homologene: 124432
Kbtbd2
Name: kelch repeat and BTB (POZ) domain containing 2
Synonyms: Bklhd1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 210973
Homologene: 17122
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Pico, Acz
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Strc
Name: stereocilin
Synonyms: DFNB16
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 140476
Homologene: 15401
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: A630082K20Rik, ENSMUSG00000073143, Jhdm1d, Kdm7a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: MJAM, 5830414C08Rik, OATP-D, Anr1, Slc21a11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108116
Homologene: 40862
Or5be3
Name: olfactory receptor family 5 subfamily BE member 3
Synonyms: Olfr1105, GA_x6K02T2Q125-48521031-48520093, MOR172-7, MOR0-6P
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258085
Homologene: 121541
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: Slc21a10, mlst-1, Slc21a6, 7330442B20Rik, Oatp1b2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28253
Homologene: 75119
Or9i14
Name: olfactory receptor family 9 subfamily I member 14
Synonyms: Olfr1499, MOR211-2, GA_x6K02T2RE5P-4147744-4146800
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258792
Homologene: 17393
Bace2
Name: beta-site APP-cleaving enzyme 2
Synonyms: ASP21, CEAP1, CDA13, ALP56, BAE2, ARP1, 1110059C24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56175
VEGA: 16
HGNC: HGNC:934
Homologene: 22696
Lrrc71
Name: leucine rich repeat containing 71
Synonyms: 4933430H15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74485
Homologene: 83646
Pctp
Name: phosphatidylcholine transfer protein
Synonyms: StarD2, PC-TP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18559
HGNC: HGNC:8752
Homologene: 32054
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
G6pd2
Name: glucose-6-phosphate dehydrogenase 2
Synonyms: G6pdx-ps1, Gpd2, Gpd-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14380
HGNC: HGNC:4057
Ncf2
Name: neutrophil cytosolic factor 2
Synonyms: NOXA2, p67phox, Ncf-2, NADPH oxidase subunit (67kDa)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17970
HGNC: HGNC:7661
Homologene: 374
Ube4bos1
Name: ubiquitination factor E4B, opposite strand 1
Synonyms: A930028C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Paqr9
Name: progestin and adipoQ receptor family member IX
Synonyms: 1700020G04Rik, C730029A08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75552
Homologene: 18882
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: IP, prostacyclin receptor
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Nhlrc1
Name: NHL repeat containing 1
Synonyms: Malin, EPM2B, B230309E09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105193
VEGA: 13
Homologene: 18439
Ccdc198
Name: coiled-coil domain containing 198
Synonyms: 1700011H14Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67082
Homologene: 49528
Senp8
Name: SUMO peptidase family member, NEDD8 specific
Synonyms: 9130010J17Rik, Nedp1, Den1, Prsc2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71599
VEGA: 9
Homologene: 14084
Vmn1r125
Name: vomeronasal 1 receptor 125
Synonyms: Gm8519
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 667215
Homologene: 104166
Calm5
Name: calmodulin 5
Synonyms: Scarf2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 494124
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 20,809,645 bp
  • T to C, chromosome 1 at 37,466,579 bp
  • T to C, chromosome 1 at 152,808,225 bp
  • T to C, chromosome 1 at 157,175,991 bp
  • T to G, chromosome 2 at 38,585,293 bp
  • C to T, chromosome 2 at 87,033,684 bp
  • T to C, chromosome 2 at 121,365,805 bp
  • T to A, chromosome 3 at 87,745,408 bp
  • A to T, chromosome 4 at 43,977,600 bp
  • A to T, chromosome 4 at 149,041,768 bp
  • A to G, chromosome 4 at 149,359,165 bp
  • A to G, chromosome 5 at 14,521,369 bp
  • G to A, chromosome 5 at 61,810,343 bp
  • C to T, chromosome 6 at 39,152,823 bp
  • A to G, chromosome 6 at 56,789,279 bp
  • A to G, chromosome 6 at 141,671,167 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • A to G, chromosome 7 at 21,272,383 bp
  • G to A, chromosome 7 at 74,504,256 bp
  • T to A, chromosome 7 at 81,308,820 bp
  • G to T, chromosome 7 at 126,572,558 bp
  • G to T, chromosome 8 at 94,307,892 bp
  • A to G, chromosome 8 at 124,675,277 bp
  • T to C, chromosome 9 at 45,976,106 bp
  • A to C, chromosome 9 at 59,750,263 bp
  • A to G, chromosome 9 at 95,560,944 bp
  • A to T, chromosome 11 at 89,988,752 bp
  • A to G, chromosome 12 at 21,332,088 bp
  • C to A, chromosome 13 at 3,854,402 bp
  • T to C, chromosome 13 at 47,014,190 bp
  • T to A, chromosome 14 at 49,234,498 bp
  • T to C, chromosome 14 at 56,691,000 bp
  • T to C, chromosome 15 at 94,638,154 bp
  • T to C, chromosome 16 at 35,697,942 bp
  • G to A, chromosome 16 at 97,421,980 bp
  • A to C, chromosome 18 at 24,507,103 bp
  • A to G, chromosome 19 at 13,815,282 bp
  • A to T, chromosome X at 151,863,959 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4563 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041788-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.