Strain Name:
C57BL/6J-MtgxR4578Btlr/Mmmh
Stock Number:
041800-MU
Citation ID:
RRID:MMRRC_041800-MU
Other Names:
R4578 (G1), C57BL/6J-MtgxR4578Btlr
Major Collection:

Gene Information

Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269523
Homologene: 5168
Gsta4
Name: glutathione S-transferase, alpha 4
Synonyms: GST 5.7, mGsta4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14860
VEGA: 9
Homologene: 135605
Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224273
Homologene: 28544
Cmtm7
Name: CKLF-like MARVEL transmembrane domain containing 7
Synonyms: Cklfsf7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102545
VEGA: 9
Homologene: 15882
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 24018
Homologene: 37851
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233833
Homologene: 41399
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Erp44
Name: endoplasmic reticulum protein 44
Synonyms: 1110001E24Rik, Txndc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76299
Homologene: 12638
Auts2
Name: autism susceptibility candidate 2
Synonyms: D830032G16Rik, A730011F23Rik, 2700063G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319974
Homologene: 22907
Ctc1
Name: CTS telomere maintenance complex component 1
Synonyms: 1500010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68964
Homologene: 11830
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208628
Homologene: 32227
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Setdb1
Name: SET domain, bifurcated 1
Synonyms: ESET, KMT1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 84505
Homologene: 32157
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Rpl27a
Name: ribosomal protein L27A
Synonyms: L27A, ribosomal protein L29 homolog (yeast), L27'
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26451
Homologene: 115477
Nat10
Name: N-acetyltransferase 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 98956
Homologene: 6785
Ccdc80
Name: coiled-coil domain containing 80
Synonyms: Urb, Ssg1, 2610001E17Rik, DRO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67896
Homologene: 12206
Cttn
Name: cortactin
Synonyms: Ems1, 1110020L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13043
HGNC: HGNC:3338
Homologene: 3834
4930522L14Rik
Name: RIKEN cDNA 4930522L14 gene
Synonyms: Gm42152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041734
Il17rb
Name: interleukin 17 receptor B
Synonyms: Evi27, IL-17ER, IL17RH1, IL-17Rh1, Il17br
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50905
Homologene: 10287
Klk12
Name: kallikrein related-peptidase 12
Synonyms: KLK-L5, 2310008B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69511
HGNC: HGNC:6360
Homologene: 44690
Srpr
Name: signal recognition particle receptor ('docking protein')
Synonyms: 1300011P19Rik, D11Mgi27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67398
Homologene: 2364
Btbd10
Name: BTB (POZ) domain containing 10
Synonyms: Gmrp1, 1110056N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68815
Homologene: 13009
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217653
Homologene: 10147
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930516E05Rik, D330038K10Rik, 4930543C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Taf6l
Name: TATA-box binding protein associated factor 6 like
Synonyms: PAF65A, C530024J06Rik, 2810417N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225895
Homologene: 4728
Scn2b
Name: sodium channel, voltage-gated, type II, beta
Synonyms: LOC214238, 2810451E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72821
VEGA: 9
Homologene: 3373
Gm7102
Name: predicted gene 7102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 633057
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: PMCA2, wms, D6Abb2e, jog, Tmy, Gena300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Elfn1
Name: leucine rich repeat and fibronectin type III, extracellular 1
Synonyms: A930017N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243312
Homologene: 18465
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Pced1a
Name: PC-esterase domain containing 1A
Synonyms: A930025D01Rik, Fam113a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319513
Homologene: 11241
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Wdr87-ps
Name: WD repeat domain 87, pseudogene
Synonyms: 4932431P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4932408N08Rik, 4931407K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77627
Homologene: 11259
Plb1
Name: phospholipase B1
Synonyms: 4632413E21Rik, 4930433E17Rik, 4930539A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665270
Homologene: 82108
Cngb3
Name: cyclic nucleotide gated channel beta 3
Synonyms: CNG6, CCNC2, Cngbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30952
HGNC: HGNC:2153
Homologene: 40908
Strc
Name: stereocilin
Synonyms: DFNB16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 140476
Homologene: 15401
Sclt1
Name: sodium channel and clathrin linker 1
Synonyms: 4931421F20Rik, 2610207F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67161
Homologene: 27031
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f1, Myhs-f, Myhsf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Duox1
Name: dual oxidase 1
Synonyms: NOXEF1, THOX1, LNOX1, 9930101G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99439
HGNC: HGNC:3062
Homologene: 68136
Tbx3
Name: T-box 3
Synonyms: D5Ertd189e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21386
Homologene: 4371
Frmd4a
Name: FERM domain containing 4A
Synonyms: C230040M21Rik, 2700017I06Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209630
Homologene: 9971
Gm5155
Name: predicted gene 5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381852
Homologene: 115938
Trim30b
Name: tripartite motif-containing 30B
Synonyms: Trim30-1, A530023O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244183
Cytip
Name: cytohesin 1 interacting protein
Synonyms: Cbp, A130053M09Rik, Pscdbp, Cybr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227929
HGNC: HGNC:9506
Homologene: 3167
Pomgnt2
Name: protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2
Synonyms: C85492, Gtdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215494
VEGA: 9
Homologene: 32795
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Kcnv2
Name: potassium channel, subfamily V, member 2
Synonyms: KV11.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240595
Homologene: 26423
Slco6b1
Name: solute carrier organic anion transporter family, member 6b1
Synonyms: 1700022M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67854
Homologene: 12199
Card14
Name: caspase recruitment domain family, member 14
Synonyms: CARMA2, Bimp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 170720
Homologene: 11469
Gm12790
Name: predicted gene 12790
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384534
Homologene: 129605
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11980
Homologene: 48402
Svop
Name: SV2 related protein
Synonyms: 1110030H18Rik, msvop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68666
Homologene: 41283
Vmn2r16
Name: vomeronasal 2, receptor 16
Synonyms: EG384220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384220
Homologene: 104825
Traf3ip2
Name: TRAF3 interacting protein 2
Synonyms: Act1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103213
HGNC: HGNC:1343
Homologene: 15885
Fam166a
Name: family with sequence similarity 166, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68222
Homologene: 12247
Coq9
Name: coenzyme Q9
Synonyms: 2310005O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67914
Homologene: 6477
Iqca
Name: IQ motif containing with AAA domain
Synonyms: 4930585L22Rik, 4930465P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74918
Homologene: 49784
Aldh3b3
Name: aldehyde dehydrogenase 3 family, member B3
Synonyms: 1700055N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73458
HGNC: HGNC:411
Homologene: 133295
Lrfn5
Name: leucine rich repeat and fibronectin type III domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238205
Homologene: 17582
Togaram1
Name: TOG array regulator of axonemal microtubules 1
Synonyms: A430041B07Rik, Fam179b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 328108
VEGA: 12
Homologene: 15025
Peli3
Name: pellino 3
Synonyms: 6030441F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240518
Homologene: 27059
Sfta2
Name: surfactant associated 2
Synonyms: EG433102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 433102
Bfar
Name: bifunctional apoptosis regulator
Synonyms: 3010001A07Rik, RNF47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67118
Homologene: 9563
Ftcd
Name: formiminotransferase cyclodeaminase
Synonyms: glutamate formiminotransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14317
HGNC: HGNC:3974
Homologene: 4848
Gfod2
Name: glucose-fructose oxidoreductase domain containing 2
Synonyms: 5730466C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70575
Homologene: 10361
Ifna14
Name: interferon alpha 14
Synonyms: OTTMUSG00000007662
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 404549
HGNC: HGNC:5425
Homologene: 68536
Igkv17-127
Name: immunoglobulin kappa variable 17-127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243433
Llph-ps2
Name: LLP homolog, pseudogene 2
Synonyms: ENSMUSG00000058791, Gm14535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100043002
Homologene: 81946
RP24-257J19.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Gm12404
Name: predicted gene 12404
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 102637977
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 90,073,750 bp
  • T to A, chromosome 1 at 96,988,697 bp
  • C to A, chromosome 2 at 4,603,679 bp
  • T to C, chromosome 2 at 25,220,288 bp
  • T to A, chromosome 2 at 58,160,012 bp
  • A to G, chromosome 2 at 103,754,072 bp
  • G to A, chromosome 2 at 121,378,003 bp
  • A to G, chromosome 2 at 122,333,777 bp
  • A to C, chromosome 2 at 130,422,676 bp
  • A to G, chromosome 3 at 19,973,888 bp
  • G to A, chromosome 3 at 41,671,465 bp
  • T to C, chromosome 3 at 75,335,243 bp
  • T to C, chromosome 3 at 95,341,395 bp
  • T to A, chromosome 4 at 19,425,613 bp
  • A to G, chromosome 4 at 33,339,050 bp
  • C to T, chromosome 4 at 41,522,014 bp
  • A to T, chromosome 4 at 42,984,565 bp
  • T to C, chromosome 4 at 48,194,595 bp
  • A to T, chromosome 4 at 88,571,510 bp
  • T to C, chromosome 4 at 101,968,127 bp
  • C to T, chromosome 5 at 32,247,557 bp
  • T to C, chromosome 5 at 67,815,815 bp
  • A to T, chromosome 5 at 109,363,799 bp
  • A to T, chromosome 5 at 109,736,671 bp
  • C to T, chromosome 5 at 114,065,682 bp
  • G to T, chromosome 5 at 119,682,776 bp
  • T to G, chromosome 5 at 123,765,955 bp
  • C to T, chromosome 5 at 132,258,934 bp
  • T to C, chromosome 5 at 139,972,053 bp
  • A to C, chromosome 6 at 48,463,373 bp
  • T to C, chromosome 6 at 67,861,199 bp
  • T to C, chromosome 6 at 113,760,711 bp
  • A to T, chromosome 7 at 10,170,690 bp
  • T to A, chromosome 7 at 17,905,152 bp
  • A to G, chromosome 7 at 29,532,781 bp
  • A to G, chromosome 7 at 43,773,243 bp
  • T to C, chromosome 7 at 104,357,331 bp
  • T to A, chromosome 7 at 109,519,900 bp
  • T to G, chromosome 7 at 113,322,752 bp
  • A to G, chromosome 7 at 123,184,221 bp
  • A to T, chromosome 7 at 144,454,716 bp
  • T to C, chromosome 8 at 94,853,606 bp
  • T to C, chromosome 8 at 105,728,246 bp
  • A to T, chromosome 8 at 110,267,339 bp
  • T to C, chromosome 9 at 35,214,608 bp
  • T to C, chromosome 9 at 45,126,162 bp
  • A to T, chromosome 9 at 78,206,020 bp
  • A to C, chromosome 9 at 114,763,283 bp
  • G to A, chromosome 9 at 121,983,065 bp
  • A to G, chromosome 10 at 39,634,654 bp
  • A to C, chromosome 10 at 76,589,258 bp
  • G to A, chromosome 10 at 79,724,448 bp
  • T to A, chromosome 11 at 67,173,258 bp
  • T to C, chromosome 11 at 69,034,910 bp
  • T to C, chromosome 11 at 79,445,759 bp
  • G to A, chromosome 11 at 119,326,741 bp
  • A to G, chromosome 12 at 51,751,932 bp
  • A to G, chromosome 12 at 61,843,977 bp
  • T to C, chromosome 12 at 65,020,326 bp
  • T to C, chromosome 12 at 65,153,881 bp
  • A to G, chromosome 14 at 30,002,399 bp
  • T to C, chromosome 15 at 81,649,009 bp
  • A to T, chromosome 15 at 83,933,168 bp
  • A to T, chromosome 16 at 13,687,443 bp
  • A to G, chromosome 16 at 45,095,486 bp
  • C to T, chromosome 16 at 59,530,201 bp
  • C to T, chromosome 17 at 35,649,883 bp
  • A to T, chromosome 19 at 3,964,832 bp
  • T to C, chromosome 19 at 4,934,458 bp
  • C to A, chromosome 19 at 8,783,971 bp
  • A to T, chromosome 19 at 16,682,110 bp
  • G to T, chromosome 19 at 27,323,594 bp
  • C to T, chromosome 19 at 61,175,926 bp
  • A to G, chromosome X at 13,218,451 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4578 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041800-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.