Strain Name:
C57BL/6J-MtgxR4614Btlr/Mmmh
Stock Number:
041825-MU
Citation ID:
RRID:MMRRC_041825-MU
Other Names:
R4614 (G1), C57BL/6J-MtgxR4614Btlr
Major Collection:

Gene Information

Gene Symbol: Mfap4 [MGI:1342276] (Mus musculus (mouse))
Name: microfibrillar-associated protein 4
Synonyms: 1110007F23Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Zfp207 [MGI:1340045] (Mus musculus (mouse))
Name: zinc finger protein 207
Synonyms: Zep, 8430401D15Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Fgfr1 [MGI:95522] (Mus musculus (mouse))
Name: fibroblast growth factor receptor 1
Synonyms: Eask, Hspy, Flt-2, Fgfr-1, FGFR-I, Fr1
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Plxnd1 [MGI:2154244] (Mus musculus (mouse))
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Washc2 [MGI:106463] (Mus musculus (mouse))
Name: WASH complex subunit 2`
Synonyms: Fam21, D6Wsu116e, C530005J20Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cep290 [MGI:2384917] (Mus musculus (mouse))
Name: centrosomal protein 290
Synonyms: b2b1752Clo, Nphp6, b2b1454Clo, Kiaa
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Setd2 [MGI:1918177] (Mus musculus (mouse))
Name: SET domain containing 2
Synonyms: KMT3A, 4921524K10Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 33,739,385 bp
  • C to T, chromosome 1 at 151,454,048 bp
  • A to G, chromosome 1 at 161,223,099 bp
  • A to T, chromosome 1 at 174,192,977 bp
  • A to G, chromosome 1 at 174,250,622 bp
  • T to G, chromosome 1 at 190,162,008 bp
  • C to A, chromosome 2 at 28,450,848 bp
  • T to C, chromosome 2 at 31,514,783 bp
  • C to A, chromosome 2 at 68,745,460 bp
  • A to T, chromosome 2 at 69,284,681 bp
  • C to T, chromosome 2 at 71,524,948 bp
  • T to C, chromosome 2 at 113,365,149 bp
  • G to A, chromosome 2 at 120,041,158 bp
  • A to T, chromosome 2 at 122,289,557 bp
  • A to T, chromosome 2 at 154,136,280 bp
  • A to G, chromosome 2 at 156,144,050 bp
  • T to C, chromosome 2 at 163,320,647 bp
  • A to G, chromosome 3 at 87,368,619 bp
  • T to A, chromosome 3 at 87,448,426 bp
  • T to G, chromosome 3 at 96,716,471 bp
  • T to A, chromosome 3 at 98,742,080 bp
  • A to T, chromosome 3 at 158,054,634 bp
  • T to A, chromosome 4 at 61,671,917 bp
  • A to G, chromosome 4 at 90,225,081 bp
  • A to G, chromosome 4 at 118,479,051 bp
  • A to T, chromosome 4 at 139,659,787 bp
  • A to G, chromosome 5 at 52,538,433 bp
  • A to T, chromosome 5 at 109,175,199 bp
  • A to T, chromosome 6 at 23,247,858 bp
  • G to T, chromosome 6 at 42,762,418 bp
  • A to G, chromosome 6 at 52,546,930 bp
  • G to A, chromosome 6 at 54,290,403 bp
  • A to G, chromosome 6 at 67,535,186 bp
  • A to T, chromosome 6 at 70,315,157 bp
  • A to G, chromosome 6 at 90,072,552 bp
  • T to C, chromosome 6 at 115,972,525 bp
  • T to A, chromosome 6 at 116,238,174 bp
  • G to A, chromosome 6 at 122,850,721 bp
  • G to A, chromosome 7 at 19,917,082 bp
  • TCCCCC to TCCCCCCC, chromosome 7 at 25,291,670 bp
  • A to T, chromosome 7 at 28,325,972 bp
  • C to T, chromosome 7 at 35,647,047 bp
  • A to T, chromosome 7 at 64,267,975 bp
  • A to T, chromosome 7 at 81,436,270 bp
  • G to A, chromosome 7 at 88,278,375 bp
  • C to T, chromosome 7 at 127,577,620 bp
  • G to A, chromosome 8 at 22,259,037 bp
  • A to G, chromosome 8 at 25,557,797 bp
  • A to G, chromosome 8 at 106,259,739 bp
  • A to T, chromosome 8 at 122,486,411 bp
  • A to C, chromosome 9 at 7,011,290 bp
  • T to A, chromosome 9 at 40,022,959 bp
  • AGAGGAGGAGGAGGAGG to AGAGGAGGAGGAGG, chromosome 9 at 58,499,432 bp
  • A to G, chromosome 9 at 63,482,581 bp
  • G to A, chromosome 9 at 65,066,396 bp
  • A to G, chromosome 9 at 65,480,389 bp
  • T to C, chromosome 9 at 110,569,813 bp
  • T to C, chromosome 10 at 100,508,740 bp
  • G to A, chromosome 10 at 100,559,687 bp
  • A to T, chromosome 10 at 107,892,124 bp
  • C to A, chromosome 11 at 61,485,509 bp
  • T to A, chromosome 11 at 80,395,190 bp
  • T to C, chromosome 11 at 87,610,935 bp
  • A to T, chromosome 11 at 90,671,126 bp
  • T to A, chromosome 11 at 98,249,777 bp
  • A to T, chromosome 11 at 101,274,111 bp
  • A to T, chromosome 12 at 31,333,945 bp
  • T to A, chromosome 12 at 65,153,529 bp
  • T to C, chromosome 12 at 70,210,979 bp
  • T to C, chromosome 12 at 72,902,485 bp
  • C to T, chromosome 12 at 114,896,243 bp
  • T to C, chromosome 12 at 115,952,660 bp
  • G to A, chromosome 13 at 49,493,841 bp
  • T to G, chromosome 13 at 77,254,256 bp
  • T to C, chromosome 14 at 50,792,195 bp
  • T to C, chromosome 14 at 51,571,585 bp
  • C to T, chromosome 14 at 52,287,467 bp
  • C to T, chromosome 15 at 73,130,967 bp
  • T to C, chromosome 15 at 75,903,576 bp
  • A to T, chromosome 15 at 77,516,572 bp
  • C to G, chromosome 15 at 78,291,702 bp
  • A to G, chromosome 16 at 32,757,058 bp
  • A to T, chromosome 16 at 96,047,359 bp
  • A to T, chromosome 17 at 35,474,020 bp
  • T to G, chromosome 17 at 36,040,537 bp
  • A to G, chromosome 17 at 54,276,559 bp
  • G to A, chromosome 17 at 75,289,994 bp
  • G to A, chromosome 18 at 37,480,345 bp
  • T to A, chromosome 18 at 43,562,592 bp
  • T to C, chromosome 19 at 8,948,128 bp
  • T to C, chromosome 19 at 10,248,277 bp
  • G to A, chromosome 19 at 39,803,856 bp
  • C to A, chromosome 19 at 44,154,134 bp
  • A to T, chromosome 19 at 55,202,147 bp
  • T to C, chromosome 19 at 55,742,796 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4614 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041825-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.