Strain Detail Sheet

Strain Name:
B6;129-Gngt1tm1Ogk/Mmmh
Stock Number:
041839-MU
Citation ID:
RRID:MMRRC_041839-MU
Other Names:
Gt-gamma KO, transducin-gamma KO

Strain Information

Gene Details

Gngt1tm1Ogk
Name: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1; targeted mutation 1, Oleg G Kisselev
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Gngt1
Name: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Synonyms: G(y)1, Gng1
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 14699
HGNC: HGNC:4411
Homologene: 7738
Genome Browser [Click image to go to Jbrowse]



Additional Resources
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Gene Ontology
BioGPS
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KOMP Phenotyping
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GeneCards
ClinGen
Genetic Alterations
The targeting construct was designed to replace all three exons with a Neo cassette to eliminate the coding region of Gngt1.
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype

Homozygous: Decline in rod visual sensitivity and severe impairment of nocturnal vision due to the reduction in signal amplification in rods, which manifests as night blindness. Slow progressive retina degeneration. Normal viability and normal fertility for both hets and homozygotes.

Heterozygous: Essentially wild type regarding signaling in rods and the absence of retina degeneration. Small physiologically inconsequential changes in transducin subunit expression in rods.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Choice Behavior/physiology
  • Female
  • GTP-Binding Protein alpha Subunits/metabolism
  • GTP-Binding Protein beta Subunits/genetics
  • GTP-Binding Protein beta Subunits/metabolism
  • GTP-Binding Protein beta Subunits/physiology
  • GTP-Binding Protein gamma Subunits/genetics
  • GTP-Binding Protein gamma Subunits/metabolism
  • GTP-Binding Protein gamma Subunits/physiology
  • Male
  • Mice
  • Mice, Knockout
  • Models, Statistical
  • Night Vision/genetics
  • Night Vision/physiology
  • Photic Stimulation
  • Retina/anatomy & histology
  • Retina/metabolism
  • Retina/physiology
  • Retina/ultrastructure
  • Retinal Rod Photoreceptor Cells/metabolism
  • Retinal Rod Photoreceptor Cells/physiology
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Transducin/metabolism
  • Vision, Ocular/genetics
  • Vision, Ocular/physiology
  • Visual Perception/genetics
  • Visual Perception/physiology
Strain Development
This strain was maintained on a mixed C57BL/6-129 background for approximately seven generations.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor
Oleg Kisselev, Ph.D., Saint Louis University.
Primary Reference

Kolesnikov AV, Rikimaru L, Hennig AK, Lukasiewicz PD, Fliesler SJ, Govardovskii VI, Kefalov VJ, and Kisselev OG, G-Protein {beta}{gamma}-Complex Is Crucial for Efficient Signal Amplification in Vision, J Neurosci. 2011 Jun 1;31(22):8067-77. (Medline PMID: 21632928)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Brown

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.